Filaments in the Microvillous Border of Intestinal Cells

McNabb, Jennifer; Sandborn, Edmund B.

doi:10.1083/jcb.22.3.701

Cited by 56 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within the microvilli, a core of several decorated filiaments can be seen, each with a r r o w h e a d complexes pointing down toward the terminal web. Cross-sections of microvilli reveal a hexagonal bundle configuration similar to that seen in intestinal microvilli [24], however, in comparison, uterine microvilli appear to contain fewer microfilaments (fig 2, inset). Following detergent treatment and S1 decoration, numerous microvilli ~- exhibit 'ballooned' apical tips, containing core microfilaments detached from the apical plasma membrane.…”

Section: Resultsmentioning

confidence: 68%

Reorganization of the apical cytoskeleton of uterine epithelial cells during early pregnancy in the rat: a study with myosin subfragment 1

Luxford

Murphy

1992

Biology of the Cell

View full text Add to dashboard Cite

Actin filaments were identified in the epithelial cells of rat uterus following detergent extraction and decoration of microfilaments (MF) with myosin subfragment 1 (S1). MF connections with cytoplasmic organelles and the apical plasma membrane are also described. Transmission electron microscopy revealed that the regular microvilli of non-pregnant, oestrous animals contain several decorated MF with rootlets descending into a densely filamentous terminal web. Following mating, the actin cytoskeleton was examined on days 1, 3 and 6 of pregnancy. In this period, the irregular projections that replace MV assumed an underlying, dense network of decorated MF, whilst smoother surfaces displayed few cytoplasmic filaments. At the time of blastocyst implantation, a structured terminal web was no longer present. Structural details were revealed concerning the contents of large, bleb-like projections found on the apical surface.

show abstract

Section: Resultsmentioning

confidence: 68%

Reorganization of the apical cytoskeleton of uterine epithelial cells during early pregnancy in the rat: a study with myosin subfragment 1

Luxford

Murphy

1992

Biology of the Cell

View full text Add to dashboard Cite

show abstract

“…With the electron microscope, the cytoplasm which contains RNA is found to contain ergastoplasm; and immediately apical to the nucleus there is a typical lamellar-vacuolar field of the Gold apparatus. A variety of axial structures have been found in microvilli from mammalian epithelia, usually either filaments or tubules (Palay & Karlin, 1959;Brown, 1962;Hanssen & Herman, 1962;Millington & Finean, 1962;Kjaerheim, 1963;McNabb & Sandborn, 1964). A wide variety of methods of fixation was used by these workers.…”

Section: Fine Structure Of Gland Cells Of Molluscsmentioning

confidence: 99%

The fine structure of digestive‐gland cells of Helix, Succinea and Testacella

Sumner

1966

Journal of the Royal Microscopical Society

View full text Add to dashboard Cite

“…This appearance suggests that the clusters may represent small portions of the "skeleton" of the free cell surface. It has been estimated in the rat that each cell bears about 1000 microvilli (24) and OsO4-fixed and sectioned material (15,18,23) suggests that the central structure in the microvillus which emerges as a root may have some attachment to the terminal web. These shadowed preparations do not indicate the nature of the attachment of the rods to one another.…”

Section: The Shadowed Fractionsmentioning

confidence: 99%

Studies on the Organization of the Brush Border in Intestinal Epithelial Cells

Overton

Eichholz

Crane

1965

The Journal of Cell Biology

View full text Add to dashboard Cite

Two of the fractions obtained by density gradient centrifugation of Tris-disrupted brush borders from hamster intestinal mucosa have been identified as the microvillus cores and their surrounding membranous coats, respectively. This identification has the following morphological basis. In shadowed preparations one fraction (cores) appears as rounded, compact rods, and thc other fraction (coats) appears as flattened shects. Both rods and sheets have dimensions appropriate to the idcntities assigncd to them. In addition, negative staining shows that thc rods are composed of aligncd particles of roughly 60 A, consistent with thc appcarance of the core in tissue scction, where 60-A fibrils are characteristic. The shccts are covered by non-aligned particles of approximately thc same size. Sectioned preparations show that the core fraction contains predominantly fibrous material with some mcmbranous contamination and that the coat fraction is apparently composcd exclusively of elongated sacs with a unit membrane structure. Some details of the structurc of thc core are evident in cases whcrc the compact rod appears to be looscned, revealing a doubled strand. The strand is approximately 350 A widc; the compact rod is roughly twice this width. With negative staining the strand shows a dense central region. Thc morphological identification presented hcrc is consistent with the distribution of enzymic activity among the density gradient fractions dcscribed in the preceding paper.

show abstract

Filaments in the Microvillous Border of Intestinal Cells

Cited by 56 publications

References 16 publications

Reorganization of the apical cytoskeleton of uterine epithelial cells during early pregnancy in the rat: a study with myosin subfragment 1

Reorganization of the apical cytoskeleton of uterine epithelial cells during early pregnancy in the rat: a study with myosin subfragment 1

The fine structure of digestive‐gland cells of Helix, Succinea and Testacella

Studies on the Organization of the Brush Border in Intestinal Epithelial Cells

Contact Info

Product

Resources

About