Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes

Singh, Shiv Mohan; Singh, Sanjay K.; Yadav, Lal Sahab; Singh, Paras Nath; Ravindra, Rasik

doi:10.14430/arctic4164

Cited by 36 publications

(27 citation statements)

References 35 publications

(29 reference statements)

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“…Generally, fungal isolates were good producers of lipase, protease and cellulase. Singh et al (2012) has reported production of amylase, cellulase, phosphatase and pectinase enzymes at 4°C and 20°C from various filamentous fungi in Ny-Alesund, Spitsbergen. Thelebolus microspore has been found a good producer of amylase, lipase and chitinase enzymes from Larsemann Hills, Antarctica (Singh et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Potential of Psychrotrophic Fungi Isolated From Siachen Glacier, Pakistan, to Produce Antimicrobial Metabolites

Hassan¹

2017

Appl Ecol Env Res

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Abstract. This is the first study of recovery and characterization of fungi from ice, sediments and water samples collected from Siachen glacier, Himalaya Range, Pakistan. The isolation and Total Viable count (CFU/ml or g) was carried out by spread plate technique at 4°C and 15°C. Seventeen fungal isolates were obtained and identified by analysis of 18S rRNA ITS region. Most frequently isolated fungal isolates were Leotiomycetes sp., followed by Thelebolus, Penicillium, Cladosporium, Trichoderma, Periconia, Geomyces, Cryptococcus and Pueraria. All isolates were found halophilic and they were able to tolerate NaCl concentration up to 10-20%. Some isolates showed viability at 45°C and most of the isolates were able to grow at pH 1-13. All isolates were screened for their antimicrobial activity against clinically isolated bacterial and fungal strains but they showed good antimicrobial activity against Gram (+) bacteria. None of the fungal isolates inhibited Gram negative clinically isolated Escherichia coli and Klebsiella pneumonia but few were able to inhibit Gram positive bacterial and fungal strains. Fungal isolates were also screened for production of extracellular enzymes (amylase, cellulase, deoxyribonuclease, lipase, phosphatase and protease). Various isolates were good producers of cellulase, lipase and protease whereas only 2 out of 17 produced DNase and 4 produced phosphatase. Keywords: non-polar glacier, psychrophilic fungi, enzymes, halophilic IntroductionPsychrophilic fungi grow optimally at 15°C or lower but can also grow at temperature around 20°C or below, while psychrotrophic fungi grow well at temperature above 20°C (Maheswari, 2005;Robinson, 2001). Such type of fungi have been found and investigated in all major cold habitats, such as Antarctica (Blanchette et al., 2010), Arctic regions (Sonjak et al., 2006) and cold deep sea environments (Damare et al., 2006). Various fungi representing different genera and species e.g. Thelebolus microspores, Lemonniera, Tetracladium, have been isolated from different regions of Himalaya, India (Sati et al., 2014;Anupama et al., 2011).The fungi in cold environments are facing numerous extreme limiting factors, including frequent freeze-thaw cycles, high salt concentration, low moisture content, extreme UV radiation and low nutrient availability (Robinson, 2001, McKenzie et al., 2003. To face such harsh conditions, fungi adapt themselves through various physiological and ecological mechanisms (Anupama et al., 2011). Although, several cold adaptive mechanisms of psychrophilic fungi have been described but it is assumed that a mixture of such mechanisms are employed by psychrophiles including production (Robinson, 2001;Ruisi et al., 2007). Psychrophilic and psychrotrophic fungi are capable of providing a large number of biotechnological and pharmaceutical applications. Psychrophilic fungi are capable of synthesizing secondary metabolites that are very unique to cold ecosystems (Rosa et al., 2008). Psychrophilic fungi are producers of cold shock and cold-acclimati...

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Section: Discussionmentioning

confidence: 99%

Potential of Psychrotrophic Fungi Isolated From Siachen Glacier, Pakistan, to Produce Antimicrobial Metabolites

Hassan¹

2017

Appl Ecol Env Res

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“…Hence, the structure, composition, and taxonomic diversity of microbial communities in the Arctic need to be studied urgently. In particular, fungi are an important proportion of microbial communities in Arctic habitats, such as soil 2 3 4 , mosses 5 , plant leaves 6 7 8 , and plant roots 9 10 11 12 . Lichens are successful colonizers of the Arctic and may play an important role in the mineral cycling patterns of Arctic habitats.…”

mentioning

confidence: 99%

Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing

Zhang

Wei

Zhang

et al. 2015

Sci Rep

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This study assessed the diversity and distribution of fungal communities associated with seven lichen species in the Ny-Ålesund Region (Svalbard, High Arctic) using Roche 454 pyrosequencing with fungal-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Lichen-associated fungal communities showed high diversity, with a total of 42,259 reads belonging to 370 operational taxonomic units (OTUs) being found. Of these OTUs, 294 belonged to Ascomycota, 54 to Basidiomycota, 2 to Zygomycota, and 20 to unknown fungi. Leotiomycetes, Dothideomycetes, and Eurotiomycetes were the major classes, whereas the dominant orders were Helotiales, Capnodiales, and Chaetothyriales. Interestingly, most fungal OTUs were closely related to fungi from various habitats (e.g., soil, rock, plant tissues) in the Arctic, Antarctic and alpine regions, which suggests that living in association with lichen thalli may be a transient stage of life cycle for these fungi and that long-distance dispersal may be important to the fungi in the Arctic. In addition, host-related factors shaped the lichen-associated fungal communities in this region. Taken together, these results suggest that lichens thalli act as reservoirs of diverse fungi from various niches, which may improve our understanding of fungal evolution and ecology in the Arctic.

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“…The exhaustion of nutrients, productions of inhibitors and depletion of oxygen causes less lipase production in larger inoculum size (Abo-State et al, 2010). Singh et al, (2012a) also reported that with the increase in inoculum size enzyme production decreased due to increase in the duration of the initial lag phase.…”

Section: Effects Of Production Parameters On Lipase By N149 and Y-32mentioning

confidence: 89%

Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica

Maharana¹,

Singh²

2018

Int.J.Curr.Microbiol.App.Sci

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Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes

Cited by 36 publications

References 35 publications

Potential of Psychrotrophic Fungi Isolated From Siachen Glacier, Pakistan, to Produce Antimicrobial Metabolites

Potential of Psychrotrophic Fungi Isolated From Siachen Glacier, Pakistan, to Produce Antimicrobial Metabolites

Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing

Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica

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