Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica

Maharana, Abhas Kumar; Singh, Shiv Mohan

doi:10.20546/ijcmas.2018.703.227

Cited by 10 publications

(4 citation statements)

References 24 publications

(33 reference statements)

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“…The effect of substrate concentrations is a crucial parameter for enzyme production which is depicted in Figure 6. An appropriate amount of substrate in a production medium not only will be utilized by the concerned microorganisms for their growth but also it can help to enhance the enzyme production effectively (Maharana and Singh, 2018c). A concentration of 2% (w/v) of skim milk was regarded as optimum for protease production by the strain N-86.…”

Section: Discussionmentioning

confidence: 99%

“…Additional carbon and mineral sources and some amount of amino acids may enhance the enzyme production capacity of a bacterial isolate by giving additional nutrients to the microorganisms for growth which in-turn enhance the enzyme production in same proportion (Maharana and Singh, 2018c). Singh and Ramana, (1998) isolated psychrotrophic Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

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Extracellular Cold Active Alkaline Proteolytic Enzyme Produced By Pseudomonas gessardii Strain N-86 Isolated from Antarctica

Maharana¹,

Singh²

2020

Int.J.Curr.Microbiol.App.Sci

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Proteases are the hydrolytic enzymes that act up on different kinds of proteins lead to formation of smaller peptides and amino acids. On the basis of action it is of exo-or endo-peptidase and it is of four types on the basis of functional groups viz. serine-, cystein-, aspartic-and metallo-protease. The protease types depend on the composition of the production medium; therefore, one microorganism can produce various types of

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Extracellular Cold Active Alkaline Proteolytic Enzyme Produced By Pseudomonas gessardii Strain N-86 Isolated from Antarctica

Maharana¹,

Singh²

2020

Int.J.Curr.Microbiol.App.Sci

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“…SSF recorded a higher pH value throughout the experiment compared to the other two treatments. According to Maharana & Singh (2018), minerals such as sodium (53.2 mg/g), potassium (47.5 mg/g), calcium (89.1 mg/g), magnesium (27.1mg/g), iron (39.4 mg/g), phosphorus and manganese have been assessed in shrimp waste powder. All shrimp waste had a neutral to slightly alkaline pH ranging from 7.02-8.3.…”

Section: Figmentioning

confidence: 99%

Application of Seaweed (Eucheuma cottonii) Extract and Shrimp Waste as Biofertilizer for Mustard (Brassica juncea L.)

Krishnamoorthy

Malek

2022

AGROTECH

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The extensive use of chemical fertilizers in agriculture poses serious collateral problems such as environmental pollution, pest resistance development and food safety decline. Researches focused on developing organic fertilizers from waste to partially replace chemical fertilizer use is increasing due to the requirement of sustainable agriculture development. Therefore, a pot experiment was carried out to study the effect of organic fertilizer made from seaweed and shrimp waste in improving crop growth and soil pH in green mustards. Three treatments were set up: seaweed and shrimp waste fertilizer (SSF), organic fertilizer (OF) and chemical fertilizer (CF). Although the SSF treatment had the highest number of leaves and plant height, ANOVA showed no significant differences between the three treatments. Soil pH in the SS treatment (pH= 7.43) was also higher than OF (pH= 6.49) and CF (pH= 7.07) though no significant differences were observed. Therefore, the fertilizer produced using seaweed and shrimp waste is comparable to other organic and chemical fertilizer and is suitable for growing leafy vegetables. The production and use of organic fertilizers made from seaweed extract and shrimp head waste can be a new wave of organic fertilizer in promoting plant growth and increasing crop yields.

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“…[4] Cold active lipases are mostly obtained from Psychrotrophic and psychrophilic microbes isolated from the Antarctic, Polar regions, and deep sea. [5,6] Being inherently non thermostable; their thermostability is generally increased by immobilization, directed evolution, protein engineering, and chemical modifications to boost their scope in industrial applications. [7][8][9] We have earlier reported the isolation of Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Response surface optimization of solvent tolerant cold active lipase production by Pseudomonas sp. VITCLP4

Lakshimi,

Kavitha

2023

Preprint

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The yield of solvent-tolerant cold active lipase from halo-tolerant Pseudomonas sp. VITCLP4 was improved by applying statistical methods to precisely combine medium components and other conditions. Two-level Plackett–Burman (PB) design was utilized to screen the most significant variables from a set of eleven variables that influence lipase production. Analysis of variance revealed that Tween-80, peptone, and KH2PO4 were statistically significant. Optimum levels of selected variables were ascertained by one-factor-at-a-time (OFAT) analysis. The optimum values and interactive effects of the selected three components were established by response surface methodology (RSM) based on the face-centered central composite design (FCCCD) using a three-level-three-factor approach. The optimized medium containing Tween-80, 0.5% (v/v), peptone, 0.75% (w/v), KH2PO4, 0.05% (w/v), yeast extract, 0.1% (w/v), olive oil, 0.2% (v/v) and inoculum size, 0.2% (v/v) resulted in maximum lipase production of 1342 Units ml− 1 min− 1 with 7.8 fold increase in the yield. This study provides insights into enhancing the lipase production statistically with optimized resources that can be utilized in bioprocess studies and industrial applications.

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Cold Active Lipases Produced by Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 Isolated from Nella Lake, Antarctica

Cited by 10 publications

References 24 publications

Extracellular Cold Active Alkaline Proteolytic Enzyme Produced By Pseudomonas gessardii Strain N-86 Isolated from Antarctica

Extracellular Cold Active Alkaline Proteolytic Enzyme Produced By Pseudomonas gessardii Strain N-86 Isolated from Antarctica

Application of Seaweed (Eucheuma cottonii) Extract and Shrimp Waste as Biofertilizer for Mustard (Brassica juncea L.)

Response surface optimization of solvent tolerant cold active lipase production by Pseudomonas sp. VITCLP4

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