Filamentous fungi in biological control: current status and future perspectives

Baron, Noemi Carla; Rigobelo, Everlon Cid; Zied, Diego Cunha

doi:10.4067/s0718-58392019000200307

“…[10]. Considered as facultative microorganisms that do not require arthropods as hosts to complete their life cycles, Metarhizium anisopliae and Beauveria bassiana are the best characterized and most employed entomopathogenic fungi in biological control programs [11].…”

Section: Introductionmentioning

confidence: 99%

“…B. bassiana implements integral protection against insect pests or constrains their growth and proliferation [12]. This fungus also exhibited dual protection ability against Rhizoctonia solani and Pythium myriotylum in B. bassiana-treated tomato seedlings [11]. Distinct studies showed that B. bassiana can be used as an effective biocontrol agent against Helicoverpa armigera in broad bean [14] Helicoverpa zea in cotton and tomato [15,16].…”

Section: Introductionmentioning

confidence: 99%

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Kuzhuppillymyal-Prabhakarankutty¹,

Ferrara-Rivero²,

Tamez‐Guerra³

et al. 2021

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Spodoptera frugiperda is a widely distributed insect pest that causes major economic losses in various crops, particularly maize. On the other hand, Beauveria bassiana is an entomopathogenic fungus that establishes symbiotic associations with many plants and contributes to tolerance against biotic and abiotic stresses. In the present work, under field conditions, 1x106 (first trial) and 1x10⁸ (second trial) of B. bassiana (GHA strain) blastospores were used for corn’s seed inoculation. In the first field trial, a higher number of larvae were present in the negative control plants in comparison with those in B. bassiana-treated plants. No larvae were found in negative control and B. bassiana-treated plants in the second field trial. In further laboratory experiments, the effects of the B. bassiana strains GHA, in addition to a native strain (PTG4) also delivered via seed treatment in maize seedlings, on S. frugiperda growth, development, and mortality were evaluated. 1x10⁶ B. bassiana blastospores were used to inoculate maize seeds, which were germinated and grown to seedlings under growth chamber conditions. Third-instar S. frugiperda larvae were allowed to feed on B. bassiana-treated and -untreated (negative control) seedlings until reaching 6th instar and transferred to artificial diet until reaching adult stage. Results showed that larvae feeding on B. bassiana strain PTG4 prolonged their larval stage. Furthermore, feeding with plants treated with B. bassiana strains yielded fewer S. frugiperda male moths and the female moths emerged with altered wings, compared with the untreated control. In conclusion, seed treatment with B. bassiana in maize reduced S. frugiperda infestation of maize plants in field trials. Besides S. frugiperda development was affected in laboratory trials.

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“…They proposed the genus Purpureocillium to accommodate the species Paecilomyces lilacinus, modifying it to Purpureocillium lilacinum, which is commonly found in soil and is one of the most studied nematophagous fungi (Baron, Rigobelo & Zied, 2019). Some authors have already studied a large number of biological nematicides based on P. lilacinum strains (Atkins et al, 2005;Dong and Zhang, 2006;Baron, Rigobelo & Zied, 2019). In many cases, these biological nematicides can replace chemical nematicides, which are nonspecific and nonselective, do not affect the development of worm eggs, are expensive, and are toxic to harmless species of invertebrates and vertebrates, including humans (Li et al, 2015;Degenkolb & Vilcinskas, 2016).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi (v0.1)"

2020

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Background. Specially on commodities crops, like soybean, maize, cotton, coffee and others, their high yields are reached mainly by the intensive use of pesticides and fertilizers. The biological management of crops is a relatively recent concept, and its application has increased expectations about a more sustainable agriculture. The use of fungi as plant bioinoculants has proven to be a useful alternative in this process, and research is deepening on genera and species with some already known potential. In this context, the present study focused on the analysis of the plant growth promotion potential of Purpureocillium lilacinum, Purpureocillium lavendulum and Metarhizium marquandii aiming its use as bioinoculants in maize, bean and soybean. Methods. Purpureocillium spp. and M. marquandii strains were isolated from soil samples. They were screened for their ability to solubilize phosphorus (P) and produce indoleacetic acid (IAA) and the most promising strains were tested at greenhouse in maize, bean and soybean plants. Growth promotion parameters including plant height, dry mass and contents of P and nitrogen (N) in the plants and in the rhizospheric soil were assessed. Results. Thirty strains were recovered and characterized as Purpureocillium lilacinum (25), Purpureocillium lavendulum (4) and Metarhizium marquandii (1). From the trial for P solubilization and IAA production, seven strains were selected and inoculated in maize, bean and soybean plants. These strains were able to modify in a different way the evaluated parameters involving plant growth in each crop, and some strains distinctly increased the availability of P and N, for the last, an uncommon occurrence involving these fungi. Moreover, the expected changes identified at the in vitro analysis were not necessarily found in planta. In addition, this study is the first to evaluate the effect of the isolated inoculation of these fungi on the growth promotion of maize, bean and soybean plants.

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“…One of these was proposed by Luangsa-ard et al (2011), who described an in-depth morphological and phylogenetic approach to Paecilomyces species. They proposed the genus Purpureocillium to accommodate the species Paecilomyces lilacinus, modifying it to Purpureocillium lilacinum, which is commonly found in soil and is one of the most studied nematophagous fungi (Baron, Rigobelo & Zied, 2019). Some authors have already studied a large number of biological nematicides based on P. lilacinum strains (Atkins et al, 2005;Dong and Zhang, 2006;Baron, Rigobelo & Zied, 2019).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi (v0.1)"

Goffré¹

2020

0

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Background. Specially on commodities crops, like soybean, maize, cotton, coffee and others, their high yields are reached mainly by the intensive use of pesticides and fertilizers. The biological management of crops is a relatively recent concept, and its application has increased expectations about a more sustainable agriculture. The use of fungi as plant bioinoculants has proven to be a useful alternative in this process, and research is deepening on genera and species with some already known potential. In this context, the present study focused on the analysis of the plant growth promotion potential of Purpureocillium lilacinum, Purpureocillium lavendulum and Metarhizium marquandii aiming its use as bioinoculants in maize, bean and soybean. Methods. Purpureocillium spp. and M. marquandii strains were isolated from soil samples. They were screened for their ability to solubilize phosphorus (P) and produce indoleacetic acid (IAA) and the most promising strains were tested at greenhouse in maize, bean and soybean plants. Growth promotion parameters including plant height, dry mass and contents of P and nitrogen (N) in the plants and in the rhizospheric soil were assessed. Results. Thirty strains were recovered and characterized as Purpureocillium lilacinum (25), Purpureocillium lavendulum (4) and Metarhizium marquandii (1). From the trial for P solubilization and IAA production, seven strains were selected and inoculated in maize, bean and soybean plants. These strains were able to modify in a different way the evaluated parameters involving plant growth in each crop, and some strains distinctly increased the availability of P and N, for the last, an uncommon occurrence involving these fungi. Moreover, the expected changes identified at the in vitro analysis were not necessarily found in planta. In addition, this study is the first to evaluate the effect of the isolated inoculation of these fungi on the growth promotion of maize, bean and soybean plants.

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Filamentous fungi in biological control: current status and future perspectives

Cited by 64 publications

References 33 publications

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Peer Review #2 of "Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi (v0.1)"

Peer Review #3 of "Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi (v0.1)"

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