Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells

Falone, Stefano; Grossi, Maria Rosaria; Cinque, Benedetta; D’Angelo, Barbara; Tettamanti, E.; Cimini, Annamaria; Ilio, Carmine Di; Amicarelli, Fernanda

doi:10.1016/j.biocel.2007.06.001

Cited by 94 publications

(80 citation statements)

References 37 publications

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“…Some available evidence showed that exposure to ELF-EMF can either enhance or inhibit cell proliferation and apoptosis. 9,10 Several studies indicated that ELF-EMF exposure leads to alteration of chromosomal regulation and structure. [11][12][13] Moreover, other in vitro studies suggested that ELF-EMF can change the expression of some protein involved in control of cell proliferation processes, 14,15 while others demonstrated that exposure to ELF-EMF has no effects on cell proliferation, DNA replication andregulation.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

et al. 2017

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Introduction: During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields (ELF-EMF) emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study. Methods: SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz (ELF-EMF) for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software. Results: Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced. Conclusion: Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation.

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Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

et al. 2017

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“…These results suggest that a 50 Hz, 1 mT magnetic field modulates the redox status of the cells. Although no major oxidative damage was detected, positive modulation of antioxidant enzyme expression, as well as a significant increase in reduced glutathione level was observed, indicating a shift of cellular environment towards a more reduced state [5]. Simultaneous increase of CAT, GR and GST activity was detected only in the K562 cell line exposed to 100 µT EMF for 24 h. These results could be a consequence of the oxidative stress adaptation.…”

Section: Discussionmentioning

confidence: 82%

“…However, the molecular mechanism through which ELF-EMF can influence cellular behavior is still unclear. A possible hypothesis is that ELF-EMF can interfere with chemical reactions involving free radical production [4] and in the last few years, some data from literature have described redox-related cellular changes, following ELF-EMF exposure [5,6]. Free radicals are known to affect various biochemical pathways and reactions including cell proliferation, cellular differentiation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Effect of ELF-EMF on antioxidant status and micronuclei in K562 cells and normal lymphocytes

et al. 2014

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The effect of ELF-EMF on DNA through changes in antioxidative enzyme activities has not been sufficiently explored yet. The aim of this study was to determine ELF-EMF effect on antioxidative enzymes in cancer cell line and genotoxic potential on normal human lymphocytes. K562 cells were exposed to 50 Hz ELF-EMF (40 μT, 100 μT; 3 h, 24 h) and spectrophotometric determination of lipid peroxidation and antioxidative enzyme activities was conducted. Genotoxicity of ELF-EMF (50 Hz, 100 μT) was investigated by cytokinesis-block micronucleus assay in a normal human lymphocytes (exposure 24 h and 48 h). Results demonstrated that ELF-EMF did not alter the process of lipid peroxidation and superoxide dismutase activity. Catalase activity was increased only after application of 100 μT EMF for 24 h. Glutathione-S-transferase and -reductase activities were increased. Treatment with 100 μT ELF-EMF (24 h, 48 h) significantly reduced micronuclei incidence, while cell proliferation was significantly increased. Results indicate that 50 Hz ELF-EMF (40 μT, 100 μT) are week stressors which alone cannot generate enough ROS to induce process of lipid peroxidation in cancer cell line but strong enough to induce response of antioxidative system. Furthermore, 100 μT ELF-EMF in human lymphocytes did not exhibit genotoxic potential during 24 h and 48 h treatment, but stimulated cell proliferation.

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“…A limited number of these studies have demonstrated that ELF-EMF promotes carcinogenic effects. Some available evidence indicates that exposure to ELF-EMF could enhance cell proliferation and inhibit apoptosis (13)(14)(15). Several studies indicated that ELF-EMFs exposure has effects on regulation and chromosomal structure (16-18).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

Rezaei‐Tavirani

Hasanzadeh

Seyyedi³

et al. 2018

Arch Neurosci

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Background: Our previous study determined the alteration in behavioral test-related memory and movement in male rats exposed to extremely low frequency electromagnetic field (ELF-EMF). The molecular mechanism of the effect of ELF-EMF on brain damage of rat has not yet fully understood, thus the current study investigated the effect of ELF-EMFs on hippocampus of male rats by the proteomics approach. The aim of this study was to investigate the effects of 50 Hz ELF-EMF at 0.5 and 1 mT 50 Hz for 2 and 4 weeks on male rats and determined protein expression of the hippocampus. Methods: Effects of 0.5 and 1 mT exposures for 2 and 4 weeks on protein expression was determined via proteomics techniques. Statistical analysis of proteome was performed using the Progensis Same Spots software. Bioinformatics analysis based on network was used for determining the mechanism. Results: Two-DE electrophoresis gels determined the effect of ELF-EMF on rat hippocampus, indicating that protein expression was overall down-regulated by increasing ELF-EMF intensity and time. The detected differentially expressed proteins between the groups comprised of Sptan1, Dpysl2, Tpi1, Lap3, Vdac1, and Tppp. Almost all were cytoskeletal proteins, most of which were increased by intensity and time. These proteins attributed to impairment in short memory of hippocampus after exposure to ELF-EMF. The GO analysis based on network, enriched two important biological process, cytoskeletal neurons and metabolic process. Conclusions: This study determined the effect of ELF-EMF, which led to change in protein expression related to the cytoskeleton that contributes to major processes in brain damage.

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Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells

Cited by 94 publications

References 37 publications

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Effect of ELF-EMF on antioxidant status and micronuclei in K562 cells and normal lymphocytes

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

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