Field production, purification and analysis of high-oleic acetyl-triacylglycerols from transgenic Camelina sativa

Liu, Jinjie; Tjellström, Henrik; McGlew, Kathleen; Shaw, Vincent; Rice, Adam; Simpson, Jeffrey P.; Kosma, Dylan K.; Ma, Wei; Yang, Wenli; Strawsine, Merissa; Cahoon, Edgar B.; Durrett, Timothy P.; Ohlrogge, John B.

doi:10.1016/j.indcrop.2014.11.019

Cited by 48 publications

(48 citation statements)

References 37 publications

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“…Camelina sativa oil is applied for production of potential adhesives containing epoxide (Kim et al, 2015) or acrylate and hydroxyl functionalities . Besides above mentioned synthetic modifications, transgenic camelina oils have been used for production of acetyl triacylglicerides, which possess lower viscosity and have improved cold temperature properties (Liu et al, 2015), and sciadonic acid (Gonzalez-Thuillier et al, 2016). In addition, camelina meal (Bullerwell et al, 2016) or its extracts (Ye et al, 2016), seeds (Bullerwell et al, 2016) and oil (Bullerwell et al, 2016;Ye et al, 2016) have planta"), coriander ("Latplanta"), ginger ("Latplanta"), oat grains (breed 'Lizete'; State Stende Cereals Breeding Institute, Latvia) and/or sprouts of barley (by-product from malt production; "Latraps", Latvia) were added to false flax oil (25 g).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties of Camelina sativa Oil and Press-Cakes

Mieriņa

Adere

Krasauska

et al. 2017

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Camelina sativa is well known due to high content of polyunsaturated fatty acids in its oil. Till now this oil has been studied mainly for applications as raw material for synthesis of resins, biodiesel and hydrocarbon fuels. This study examines the oxidative stability of cold-pressed Camelina sativa (also known as camelina, false flax or gold-of-pleasure) oil and its extracts of spices. Despite the high content of polyunsaturated fatty acids, Camelina sativa oil appeared more rigid against oxidation than rapeseed or flax oil. Extracts of different spices were prepared by maceration in camelina oil at room temperature for 24 h. The stability of extracts was determined under accelerated oxidation conditions and monitored by peroxide values. Most of the tested additives (e.g., bay leaves, allspice, clove, barley sprouts, coriander, ginger) did not influence or even decreased oxidative stability of the oil. However, oil with thyme additive demonstrated remarkably higher stability then Camelina sativa oil alone. Press-cakes of camelina seeds were extracted with two polar solvents (ethanol or water) and their mixtures under variable conditions (room temperature or reflux). Prepared polar extracts of press-cakes were characterised by total polyphenol content (Folin–Ciocalteu method) and antiradical activity against 1,1-diphenyl-2-picryl hydrazyl and galvinoxyl.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Properties of Camelina sativa Oil and Press-Cakes

Mieriņa

Adere

Krasauska

et al. 2017

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…Moreover, the seed yield is adequate to facilitate the rapid production of oil in quantities large enough for small-scale testing and product development. In recent years, several different oil traits have been developed in C. sativa seeds, such as high 18:1n-9 (Kang et al, 2011;, high 16:0 , and high v-7 fatty acids (Nguyen et al, 2015), as well as the production of diverse extrinsic lipid compounds, such as wax esters (Iven et al, 2016), fish oils (Petrie et al, 2014;Ruiz-Lopez et al, 2014), nervonic acid (Huai et al, 2015), and acetyl glyceride oils (Liu et al, 2015).…”

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confidence: 99%

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

et al. 2017

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ORCID IDs: 0000-0001-6929-7859 (S.M.); 0000-0003-3152-0394 (D.S.); 0000-0001-8255-3255 (C.H.); 0000-0003-0489-3072 (K.C.); 0000-0002-9888-7003 (I.F.).Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) or PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) on seed lipid composition were assessed in several Camelina sativa lines. Furthermore, in vitro assays of acyltransferases in microsomal fractions prepared from developing seeds of some of these lines were performed. Decreased expression of DGAT1 led to an increased percentage of 18:3n-3 without any change in total lipid content of the seed. The tri-18:3 TAG increase occurred predominantly in the cotyledon, as determined with matrix-assisted laser desorption/ionization-mass spectrometry, whereas species with two 18:3n-3 acyl groups were elevated in both cotyledon and embryonal axis. PDAT overexpression led to a relative increase of 18:2n-6 at the expense of 18:3n-3, also without affecting the total lipid content. Differential distributions of TAG species also were observed in different parts of the seed. The microsomal assays revealed that C. sativa seeds have very high activity of diacylglycerol-phosphatidylcholine interconversion. The combination of analytical and biochemical data suggests that the higher 18:2n-6 content in the seed oil of the PDAT overexpressors is due to the channeling of fatty acids from phosphatidylcholine into TAG before being desaturated to 18:3n-3, caused by the high activity of PDAT in general and by PDAT specificity for 18:2n-6. The higher levels of 18:3n-3 in DGAT1-silencing lines are likely due to the compensatory activity of a TAG-synthesizing enzyme with specificity for this acyl group and more desaturation of acyl groups occurring on phosphatidylcholine.

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“…This scenario has already been successfully demonstrated with the production of high-oleic acetyl-TAGs [71]. High oleic Camelina lines generated using RNAi against CsFAD2 and CsFAE1 [28] were transformed with EaDAcT and RNAi against DGAT1 and PDAT.…”

Section: Enhancing the Functionality Of Acetyl-tagsmentioning

confidence: 95%

“…The resultant lines produced up to 70 mol% acetyl-TAGs. Further, 3-acetyl-1,2-dioleoyl-sn-glycerol was the most abundant acetyl-TAG molecular species, comprising 47% of all acetyl-TAG molecular species [71]. The high proportion of oleic acid in the sn-1 and sn-2 positions of the acetyl-TAGs changed the physical properties of these molecules.…”

Section: Enhancing the Functionality Of Acetyl-tagsmentioning

confidence: 98%

“…Indeed, acetyl-TAGs possess a viscosity in the range of Diesel #4, a heavier grade diesel used in the constant speed engines found in locomotives, ships and heavy generators. Further, acetylTAGs also possess improved cold temperature properties compared to regular TAGs [70,71], an important consideration, especially in colder climates. In addition to being used as a fuel, these properties of acetyl-TAGs lend themselves to other useful applications such as biodegradable lubricants, transformer oils and other products.…”

Section: Production Of High Levels Of Acetyl-tags In Camelinamentioning

confidence: 99%

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Camelina sativa: An ideal platform for the metabolic engineering and field production of industrial lipids

Bansal

Durrett

2016

Biochimie

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Triacylglycerols (TAG) containing modified fatty acids with functionality beyond those found in commercially grown oil seed crops can be used as feedstocks for biofuels and bio-based materials. Over the years, advances have been made in transgenically engineering the production of various modified fatty acids in the model plant Arabidopsis thaliana. However, the inability to produce large quantities of transgenic seed has limited the functional testing of the modified oil. In contrast, the emerging oil seed crop Camelina sativa possesses important agronomic traits that recommend it as an ideal production platform for biofuels and industrial feedstocks. Camelina possesses low water and fertilizer requirements and is capable of yields comparable to other oil seed crops, particularly under stress conditions. Importantly, its relatively short growing season enables it to be grown as part of a double cropping system. In addition to these valuable agronomic features, Camelina is amenable to rapid metabolic engineering. The development of a simple and effective transformation method, combined with the availability of abundant transcriptomic and genomic data, has allowed the generation of transgenic Camelina lines capable of synthesizing high levels of unusual lipids. In some cases these levels have surpassed what was achieved in Arabidopsis. Further, the ability to use Camelina as a crop production system has allowed for the large scale growth of transgenic oil seed crops, enabling subsequent physical property testing. The application of new techniques such as genome editing will further increase the suitability of Camelina as an ideal platform for the production of biofuels and bio-materials.

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Field production, purification and analysis of high-oleic acetyl-triacylglycerols from transgenic Camelina sativa

Cited by 48 publications

References 37 publications

Antioxidant Properties of Camelina sativa Oil and Press-Cakes

Antioxidant Properties of Camelina sativa Oil and Press-Cakes

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

Camelina sativa: An ideal platform for the metabolic engineering and field production of industrial lipids

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