Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis

Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring

“…kurstaki. Incorporation of the B. thuringiensis Cry1Ab toxin gene into maize germplasm (Koziel et al, 1993) results in expression that controls O. nubilalis larvae (Walker et al, 2000). High selection pressure and adaptation to transgenic toxins by O. nubilalis larvae raises concerns regarding sustainability of Bt technology.…”

Insect Resistance in Transgenic Chrysanthemum [Dendranthema * grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified .DELTA.-endotoxin Gene of Bacillus thuringiensis

“…The proteins were separated on 10.0 % SDS-PAGE gels (Laemmli 1970) and blotted onto a PVDF membrane (MICRON SEPARATIONS Inc.) using a semidry transfer cell (BioRad). Western immunoblot analysis was performed according to the alkaliphosphatase procedure of Koziel et al (1993). The purification of an antigen, Cry1Ab ICP, was modified from the method of Mochizuki (1994).…”

“…To increase the expression level of the Bt genes in transgenic plants, Bt genes have been extensively modified, fused to highly efficient promoters and introduced into tobacco (Perlak et al 1991, Sutton et al 1992, tomato (Perlak et al 1991), potato (Adang et al 1993), cotton (Perlak et al 1990), rice (Fujimoto et al 1993) and maize (Koziel et al 1993). To achieve a high expression of these prokaryotic genes in higher plants, modification of the coding sequence, such as removal of ATTTA sequences and use of the preferred codons, was undertaken.…”

“…Modification of the coding sequences successfully enhanced plant expression of Bt genes (Perlak et al 1990, Sutton et al 1992, Adang et al 1993, Koziel et al 1993.…”

Molecular and functional characterization ofcry1Actransgenic pea lines

“…Koul et al, 2014 Total larval mortality of 2 insect pests (Trichoplusia ni and Spodoptera exigua ) and high levels of plant protection have been observed on transgenic cotton plants expressing cry1Ac or cry1Ab genes from Bt var Kurstaki (HD-1 and HG-73, respectively). Perlak et al, 1990 Different cry toxins (cry1Ab, cry1ac or cry1B) have also been expressed in cereal crops like rice Cheng, Sardana, Kaplan, Altosaar 1998;Datta et al, 1998;Breitler 2001, Khanna, Raina 2002Ramesh, Nagadhara, Reddy, Rao 2004, maize Bohorova et al, 1999Armstrong 1995, Koziel et al, 1993and sorghum.Girijashankar et al, 2005 According to the reports, up to 100% larval mortality of the target insect pests as well as substantially reduced plant damage was observed on the transgenic plants of these crops.…”