Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring

Coates, Brad S.; Sumerford, Douglas V.; Hellmich, Richard L.; Lewis, Leslie C.

doi:10.1016/j.ibmb.2004.10.008

Cited by 19 publications

(28 citation statements)

References 52 publications

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“…Single-locus STS markers were shown by pedigree analysis for O. nubilalis cadherin (Coates et al 2005a), ommochrome binding proteins (obp1 and obp2) (Coates et al 2005b), brainiac/bre5 (Coates et al 2007), and aminopeptidase 1 (APN1) (Coates et al 2008). Additional single locus markers were from OnZ1 and OnW1 (Coates and Hellmich 2003) and kettin (ket), triosphosphate isomerase (tpi), and lactose dehydrogenase (ldh) (Dopman et al 2005;Malausa et al 2007).…”

Section: Marker Development and Onb1 Pcr Screeningmentioning

confidence: 99%

“…Final PCR amplification was 35 cycles of 96 8C for 20 s, 50 8C for 30 s, and 72 8C for 1 min. PCR products for O. nubilalis OnZ1 and OnW1,cadherin,obp1,obp2, and bre5 took place according to Coates and Hellmich (2003) and Coates et al (2005aCoates et al ( , 2005bCoates et al ( ), 2007, respectively. Entire PCR product volumes were separated on 1.5% agarose gels and positive BAC SPs or RPs was identified by presence-absence of the gene fragment compared with positive control DNA.…”

Section: Marker Development and Onb1 Pcr Screeningmentioning

confidence: 99%

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Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research

Coates

Sumerford

Hellmich

et al. 2009

Genome

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The European corn borer, Ostrinia nubilalis, is a serious pest of food, fiber, and biofuel crops in Europe, North America, and Asia and a model system for insect olfaction and speciation. A bacterial artificial chromosome library constructed for O. nubilalis contains 36 864 clones with an estimated average insert size of >or=120 kb and genome coverage of 8.8-fold. Screening OnB1 clones comprising approximately 2.76 genome equivalents determined the physical position of 24 sequence tag site markers, including markers linked to ecologically important and Bacillus thuringiensis toxin resistance traits. OnB1 bacterial artificial chromosome end sequence reads (GenBank dbGSS accessions ET217010 to ET217273) showed homology to annotated genes or expressed sequence tags and identified repetitive genome elements, O. nubilalis miniature subterminal inverted repeat transposable elements (OnMITE01 and OnMITE02), and ezi-like long interspersed nuclear elements. Mobility of OnMITE01 was demonstrated by the presence or absence in O. nubilalis of introns at two different loci. A (GTCT)n tetranucleotide repeat at the 5' ends of OnMITE01 and OnMITE02 are evidence for transposon-mediated movement of lepidopteran microsatellite loci. The number of repetitive elements in lepidopteran genomes will affect genome assembly and marker development. Single-locus sequence tag site markers described here have downstream application for integration within linkage maps and comparative genomic studies. Résumé : La pyrale du maïs, Ostrinia nubialis, est un ravageur important des cultures pour des fins alimentaires, textiles et énergétiques en Europe, en Amérique du Nord et en Asie. De plus, il s'agit d'une espèce modèle pour l'olfaction et la spéciation chez les insectes. Une banque de chromosomes bactériens artificiels a été produite pour l'O. nubialis et totalise 36 864 dont la taille moyenne des inserts est d'environ 120 kb pour une couverture génomique de 8,8 fois. Un criblage de clones OnB1 conférant une couverture de 2,76 équivalents génomiques a permis de déterminer la position physique de 24 marqueurs de séquence connue dont des marqueurs liés à des caractères écologiques importants ainsi qu'à la résistance à la toxine du Bacillus thuringiensis. Les séquences des extrémités de clones chromosomes bactériens artificiels OnB1 (accessions GenBank dbGSS ET217010 à ET217273) présentaient de l'homologie avec des gènes annotés ou des étiquettes de séquences exprimées et ont permis d'identifier des séquences répétitives : des éléments transposables miniatures à répé-titions subterminales inversées (OnMITE01 et OnMITE02) ainsi que des éléments nucléaires dispersés de grande taille) de type ezi. La mobilité des éléments OnMITE01 a été démontrée par leur présence ou absence au sein d'introns chez deux locus de l'O. nubialis. Un motif tétranucléotidique répété (GTCT)n aux extrémités 5' des éléments OnMITE01 et On-MITE02 fournit la preuve de la mobilité de locus microsatellites chez les insectes via le mouvement de transposons. Le nombre d'élé...

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Section: Marker Development and Onb1 Pcr Screeningmentioning

confidence: 99%

Section: Marker Development and Onb1 Pcr Screeningmentioning

confidence: 99%

Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research

Coates

Sumerford

Hellmich

et al. 2009

Genome

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“…Genomic DNA remaining after AFLP template preparation was diluted to *10 ng/ll with deionize nucleasefree water. SNP markers previously developed for the candidate Bt-resistance genes brainiac (brn), cadherin (cad), and aminopeptidase N (apn1 and apn3) were used to genotype initial parents, FQ4 and FQ5 backcross parents, and subsequent backcross progeny reared on untreated control diet, using PCR-RFLP methods described by Coates et al (2005Coates et al ( , 2007Coates et al ( , 2008a. Additionally, locusspecific markers for a larval midgut-expressed membranebound alkaline phosphatase (malp) were PCR-amplified with mALP-F3 (5 0 -CCA GCG CAA CGG CCA GAC-3 0 ) and mALP-R3d1 (5 0 -GAT TT GA GC GT CC AG GCA ATA-3 0 ) using conditions described by Coates (personal comm.).…”

Section: An Integrated O Nubilalis Aflp-and Snp-based Linkage Mapmentioning

confidence: 99%

“…Compared to candidate gene approaches that have investigated the inheritance of Bt toxin-resistance traits (Coates et al 2005(Coates et al , 2007(Coates et al , 2008a, QTL analysis detect the co-segregation of anonymous markers. Candidate gene studies are limited in scope due to a focus upon one to few loci, and assume that similar traits evolve by analogous biochemical mechanisms.…”

Section: Mapping Of the Larval O Nubilalis Cry1f Resistance Traitmentioning

confidence: 99%

“…Previous investigation of O. nubilalis larval resistance to the Bt toxin Cry1Ab used SNP markers to track the segregation of the candidate genes aminopeptidase N, brainiac, and cadherin among full-sib F 1 s, but was unable to correlate the inheritance of alleles from resistant parents with the resistance traits (Coates et al 2005(Coates et al , 2007(Coates et al , 2008a. These studies highlighted the limitations of investigating a small number of genomic loci, and of the need for genomewide scans to effectively isolate quantitative trait loci (QTL).…”

Section: Introductionmentioning

confidence: 99%

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A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes

et al. 2011

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The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance. Abstract The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae

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A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab

Ferry

Wang

et al. 2013

Transgenic Res

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A Cry1Ab-resistant population of Asian corn borer (ACB-AbR) exhibiting approximately 100 times greater resistance to activated Cry1Ab than a susceptible population (Ostrinia furnacalis; ACB-BtS), was previously shown to exhibit high levels of cross-resistance to Cry1Ah (131-fold), but no cross-resistance to Cry1Ie. It was suggested that the proposed mechanism of resistance was due to the alteration of specific receptors for Cry toxins in the midgut brush border membrane. In the present study a proteomic-based approach was used to identify proteins from brush border membrane vesicles (isolated from both resistant and susceptible Ostrinia furnacalis larvae) interacting with biotinylated Cry1Ab, Cry1Ah, and Cry1Ie. 2D-Electrophoresis in combination with ligand blots were employed and putative protein identities obtained using MALDI-ToF/ToF mass spectrometry. The V-type proton ATPase catalytic subunit A and heat shock 70 kDa proteins were identified as interacting with the Cry toxins tested in the ACB-AbR and ACB-BtS larvae. The biotinylated Cry toxins showed markedly stronger interactions with proteins in the resistant compared to the susceptible larvae, suggesting an up-regulation of the V-type proton ATPase catalytic subunit A and heat shock 70 kDa proteins in the resistant (ACB-AbR) larvae. Interestingly, Cry1Ie interactions with the V-type proton ATPase catalytic subunit A in the ACB-BtS larvae appeared to be absent.

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Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring

Cited by 19 publications

References 52 publications

Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research

Repetitive genome elements in a European corn borer, Ostrinia nubilalis, bacterial artificial chromosome library were indicated by bacterial artificial chromosome end sequencing and development of sequence tag site markers: implications for lepidopteran genomic research

A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes

A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab

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