Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

Rouleau, Isabelle; Charest, Hugues; Douville-Fradet, Monique; Skowronski, Danuta M.; Serres, Gaston De

doi:10.1128/jcm.00762-09

Cited by 26 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall sensitivities of the QuickVue Influenza AϩB and BinaxNOW Influenza A&B tests were 82% and 71%, respectively. The findings agree with those of other studies on these RIDTs using NPA specimens, which contain more epithelial cells, as specimens for testing (pooled sensitivities of 19.5 to 85.7% for the QuickVue Influenza AϩB test [14][15][16][17] and 21.6 to 71.0% for the BinaxNOW Influenza A&B test [14,[18][19][20]). Notably, the sensitivity revealed in our population of hospitalized patients is among the highest reported for RIDTs.…”

Section: Discussionsupporting

confidence: 82%

Clinical and Virologic Factors Associated with Reduced Sensitivity of Rapid Influenza Diagnostic Tests in Hospitalized Elderly Patients and Young Children

et al. 2014

View full text Add to dashboard Cite

Rapid influenza diagnostic tests (RIDTs) are commonly used by clinicians to guide patient management. Data on sensitivities among hospitalized patients are limited. Here, we evaluated the clinical and virologic factors affecting the sensitivities of 2 commercially available RIDTs (BinaxNOW Influenza A&B and QuickVue Influenza A؉B) on nasopharyngeal aspirate (NPA) specimens collected from elderly patients and young children hospitalized for influenza. Influenza cases and age-matched negative controls were prospectively enrolled during the 2011-2012 influenza season in Hong Kong. NPA specimens were collected at presentation before antiviral treatment. Real-time reverse transcription-PCR (RT-PCR) results were used as references for the sensitivity analyses. One hundred patients (57 influenza cases and 43 controls) were studied. Both RIDTs had 100% specificities. The sensitivities of the BinaxNOW Influenza A&B and QuickVue Influenza A؉B tests were 70% and 82%, respectively. For both tests, the sensitivities were lower in cases with presentation times beyond 2 days of illness onset than for those within this time (50 to 71% versus 85 to 91%, respectively). There were trends toward lower sensitivities for influenza B than for influenza A (66 to 81% versus 76 to 84%, respectively), among young children than among the elderly patients (63 to 78% versus 80 to 88%, respectively), and among cases with pneumonia than those without pneumonia (75% versus 82 to 94%, respectively). The sensitivities of the RIDTs decreased with reduced NPA viral RNA levels (5.6 to 15.0% reduction per 1-log decrease), which declined progressively after illness onset (Spearman's rho, ؊0.47 [P < 0.05] and ؊0.66 [P < 0.001] for influenza A and B, respectively). Collectively, late presentation, a low NPA viral load, and probably lower respiratory manifestation are factors associated with reduced sensitivities of RIDTs for diagnosing influenza in hospitalized patients. A negative RIDT result should be interpreted with caution.

show abstract

Section: Discussionsupporting

confidence: 82%

Clinical and Virologic Factors Associated with Reduced Sensitivity of Rapid Influenza Diagnostic Tests in Hospitalized Elderly Patients and Young Children

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Furthermore, positivity rates were higher in children than in adults, as has been reported before (9,13,15,17). The test was designed for use on nasal swabs, and, indeed, nasal swabs had a much higher diagnostic yield than throat swabs (73.6% versus 52.4%); this has also been reported for other POC tests (14), and, furthermore, although positivity rates for RT-PCR in nasal and throat swabs were similar, median C T values in positive throat swabs were higher than those in positive nasal swabs.…”

Section: Discussionsupporting

confidence: 65%

Clinical Validation of a Point-of-Care Multiplexed In Vitro Immunoassay Using Monoclonal Antibodies (the MSD Influenza Test) in Four Hospitals in Vietnam

et al. 2012

View full text Add to dashboard Cite

“…4 Our results of the sensitivity of physicianordered rapid influenza tests (66% among inpatients and 56% among outpatients) fall within the previously reported range. [32][33][34][35] Rapid influenza tests can provide clinically useful results in a timely fashion. However, many factors can influence the accuracy of rapid influenza test results including the pretest probability of influenza based on presenting clinical signs and symptoms, the local prevalence of influenza, the duration of illness, the quality and type of specimen collected, and the criterion standard to which the rapid test is compared.…”

Section: Figurementioning

confidence: 99%

The Burden of Influenza in Young Children, 2004–2009

Poehling

Edwards²,

Griffin³

et al. 2013

Pediatrics

157

View full text Add to dashboard Cite

OBJECTIVE: To characterize the health care burden of influenza from 2004 through 2009, years when influenza vaccine recommendations were expanded to all children aged ≥6 months. METHODS: Population-based surveillance for laboratory-confirmed influenza was performed among children aged <5 years presenting with fever and/or acute respiratory illness to inpatient and outpatient settings during 5 influenza seasons in 3 US counties. Enrolled children had nasal/throat swabs tested for influenza by reverse transcriptase-polymerase chain reaction and their medical records reviewed. Rates of influenza hospitalizations per 1000 population and proportions of outpatients (emergency department and clinic) with influenza were computed. RESULTS: The study population comprised 2970, 2698, and 2920 children from inpatient, emergency department, and clinic settings, respectively. The single-season influenza hospitalization rates were 0.4 to 1.0 per 1000 children aged <5 years and highest for infants <6 months. The proportion of outpatient children with influenza ranged from 10% to 25% annually. Among children hospitalized with influenza, 58% had physician-ordered influenza testing, 35% had discharge diagnoses of influenza, and 2% received antiviral medication. Among outpatients with influenza, 7% were tested for influenza, 7% were diagnosed with influenza, and <1% had antiviral treatment. Throughout the 5 study seasons, <45% of influenza-negative children ≥6 months were fully vaccinated against influenza. CONCLUSIONS: Despite expanded vaccination recommendations, many children are insufficiently vaccinated, and substantial influenza burden remains. Antiviral use was low. Future studies need to evaluate trends in use of vaccine and antiviral agents and their impact on disease burden and identify strategies to prevent influenza in young infants.

show abstract

Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

Cited by 26 publications

References 25 publications

Clinical and Virologic Factors Associated with Reduced Sensitivity of Rapid Influenza Diagnostic Tests in Hospitalized Elderly Patients and Young Children

Clinical and Virologic Factors Associated with Reduced Sensitivity of Rapid Influenza Diagnostic Tests in Hospitalized Elderly Patients and Young Children

Clinical Validation of a Point-of-Care Multiplexed In Vitro Immunoassay Using Monoclonal Antibodies (the MSD Influenza Test) in Four Hospitals in Vietnam

The Burden of Influenza in Young Children, 2004–2009

Contact Info

Product

Resources

About