Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection

Buendía, A. J.; Cuello, F.; Rı́o, Laura Del; Gallego, María Soledad Carrillo; Caro, M.R.; Salinas, J.

doi:10.1016/s0378-1135(00)00298-4

Cited by 44 publications

(38 citation statements)

References 24 publications

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“…This suggests that the lack of specificity of CFT in the field may be due to the arthritogenic and conjunctival subtypes rather than the more common enteric subtype, as suggested previously (19,22). CFT also performed better than has been reported by other groups, in which the sensitivity has been reported to be lower with either naturally or experimentally infected sheep (5,19,25), although six borderline positive samples and two negative samples were obtained from ewes that either aborted or lambed with heavily infected placentas (group 1A). The specificity of CFT and the variability of CFT antibody titers, in which very low or borderline titers are produced in some animals following abortion, reinforce the arguments that the test should be used only for flocks and not for the diagnosis of infection in individual animals (30).…”

Section: Discussionmentioning

confidence: 48%

“…In view of the poor specificity of CFT with the sera from animals experimentally infected (group 3) and naturally infected (group 6) with C. pecorum compared to the results of the rOMP90-4 iELISA, the results obtained with the group 7 sera indicate that the flocks are likely to be infected with both C. pecorum and C. abortus. Such mixed infections have been suggested to be a common occurrence in flocks (5). The CFT results with the group 3 and 6 sera also suggest that the ewes in group 5, which had no clinical history of abortion but which had large numbers of samples seropositive by CFT, are infected with C. pecorum and that this infection is more likely due to an arthritogenic or conjunctival subtype rather than an enteric subtype.…”

Section: Discussionmentioning

confidence: 76%

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Serological Diagnosis of Ovine Enzootic Abortion by Enzyme-Linked Immunosorbent Assay with a Recombinant Protein Fragment of the Polymorphic Outer Membrane Protein POMP90 ofChlamydophila abortus

et al. 2002

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Ovine enzootic abortion (OEA) resulting from infection of sheep and goats with Chlamydophila abortus is of major economic importance worldwide. Over the last 50 years the serological diagnosis of infection has been based mainly on the complement fixation test (CFT), which lacks both sensitivity and specificity because of cross-reactive antibodies to other gram-negative bacteria, including another common chlamydial pathogen of sheep, Chlamydophila pecorum. In the present study, a series of overlapping recombinant antigens representing the polymorphic outer membrane protein POMP90 of C. abortus was assessed by enzyme-linked immunosorbent assay (ELISA) with a panel of 143 serum samples from sheep experimentally infected with C. abortus, from sheep clinically free of OEA, and from specific-pathogen-free lambs experimentally infected with different subtypes of C. pecorum. The results were compared to those obtained by CFT and another recently described test, an indirect ELISA (iELISA) with the recombinant OMP91B (rOMP91B) fragment (rOMP91B iELISA) (D. Longbottom, E. Psarrou, M. Livingstone, and E. Vretou, FEMS Microbiol. Lett. 195:157-161, 2001). The rOMP90-3 and rOMP90-4 ELISAs were identified as being more sensitive and specific than CFT. Assays with both fragments were evaluated further with a panel of 294 field serum samples from flocks with documented histories of abortion, from flocks with no clinical histories of abortion but which had a high proportion of samples seropositive by CFT, and from animals with no histories of abortion but from which various C. pecorum subtypes had been isolated. ELISAs with both POMP90 fragments outperformed CFT with serum samples from C. pecorum-infected animals, producing no false-positive results. However, the ELISA with the rOMP90-4 fragment appeared to be more sensitive than the one with rOMP90-3, as it identified more of the OEA-positive samples. The ELISA with the rOMP90-4 fragment was also able to identify apparently healthy animals that were infected with an enteric strain of C. abortus in flocks that were probably infected with both enteric C. abortus and C. pecorum strains. The identification of animals infected with enteric C. abortus is extremely important in controlling the spread of OEA. Overall, the new rOMP90-4 ELISA was found to be a more sensitive and specific test than CFT for differentiating animals infected with C. abortus from those infected with C. pecorum.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 76%

Serological Diagnosis of Ovine Enzootic Abortion by Enzyme-Linked Immunosorbent Assay with a Recombinant Protein Fragment of the Polymorphic Outer Membrane Protein POMP90 ofChlamydophila abortus

et al. 2002

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“…Em bovinos, a resposta humoral desenvolvida nas infecções por C. abortus são IgG1 e IgG2, mas somente a IgG1 (presente nas primeiras 3-4 semanas pós-infecção) fixa o complemento de cobaio (Schmeer et al 1987, Twomey et al 2006. Apesar de suas limitações, a prova de fixação de complemento tem sido utilizada em países onde existem programas de controle de aborto por C. abortus (Buendía et al 2001, Longbottom & Coulter 2003.…”

Section: Introductionunclassified

Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná

et al. 2009

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“…Indirect ELISA for detection of chlamydial antibodies in sheep was developed mostly for research purposes Kaltenboeck et al 1997;Salti-Montesanto et al 1997;Gut-Zangger et al 1999;Kennedy et al 2001;Longbottom et al 2001 andBuendia et al 2001). Nowadays, iELISA kits are available commercially for detection of antibodies against Chlamydophila abortus in animals (Pospí‰il et al 1996;VûÏník andPospí‰il 1997 andBuendia et al 2001).…”

mentioning

confidence: 99%

Detection of IgG Antibodies against Chlamydophila abortus in Sheep with Reproductive Disorders

Trávníček¹,

Kováčová²,

Bhide³

et al. 2003

Acta Vet. Brno

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Chlamydophila (Chl.) abortus is the most important causative agent of the enzootic abortion and other chlamydial infections of sheep. Complement fixation (CF) test is a very useful serological method as screening test, but the diagnosis can be complicated by non-specific, false positive reactions with some Gram-negative bacteria as well as with Chl. pecorum. Diagnosis can be improved by simultaneous use of indirect (i)ELISA. Moderate agreement (Kappa -0.568) between CF test and iELISA was observed in this study. We observed 36.0 % (75/208) positive samples from clinically healthy sheep and 63.2 % (91/144) in the sheep with reproductive disorders by detecting anti-Chl. abortus IgG antibodies using iELISA. When the same sera samples were investigated by CF test, only 13.9 % seropositivity was observed in clinically health sheep whereas in the group with reproductive disorders, positivity observed was 45.8%. Sensitivity and specificity of iELISA used in this study was found to be higher than CF test.

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Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection

Cited by 44 publications

References 24 publications

Serological Diagnosis of Ovine Enzootic Abortion by Enzyme-Linked Immunosorbent Assay with a Recombinant Protein Fragment of the Polymorphic Outer Membrane Protein POMP90 ofChlamydophila abortus

Serological Diagnosis of Ovine Enzootic Abortion by Enzyme-Linked Immunosorbent Assay with a Recombinant Protein Fragment of the Polymorphic Outer Membrane Protein POMP90 ofChlamydophila abortus

Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná

Detection of IgG Antibodies against Chlamydophila abortus in Sheep with Reproductive Disorders

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