Field-Effect Transistor with a Plasmonic Fiber Optic Gate Electrode as a Multivariable Biosensor Device

Hasler, Roger; Reiner‐Rozman, Ciril; Fossati, Stefan; Aspermair, Patrik; Dostálek, Jakub; Lee, Seungho; Ibáñez, María; Bintinger, Johannes; Knoll, Wolfgang

doi:10.1021/acssensors.1c02313

Cited by 21 publications

(26 citation statements)

References 43 publications

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“…This result indicates a significant increase in surface mass density of the HD22 aptamer on the PEDOT-N 3 film compared to previously reported surface architectures based on PEG-thiol self-assembled monolayers or polymer brushes. 89 , 90 This result can be attributed tentatively to the porous structure of the polymer film and the associated high surface area with a high density of clickable groups. After the washing of the surface with PBS, the immersion of the aptamer-modified fiber in a 200 nM thrombin solution in PBS results in a resonance wavelength shift of 3.0 ± 0.3 nm due to the recognition and binding of the cardiac biomarker by the aptamer-modified fiber tip.…”

Section: Resultsmentioning

confidence: 93%

“Clickable” Organic Electrochemical Transistors

Fenoy

Hasler

Quartinello³

et al. 2022

JACS Au

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Interfacing the surface of an organic semiconductor with biological elements is a central quest when it comes to the development of efficient organic bioelectronic devices. Here, we present the first example of “clickable” organic electrochemical transistors (OECTs). The synthesis and characterization of an azide-derivatized EDOT monomer (azidomethyl-EDOT, EDOT-N3) are reported, as well as its deposition on Au-interdigitated electrodes through electropolymerization to yield PEDOT-N3-OECTs. The electropolymerization protocol allows for a straightforward and reliable tuning of the characteristics of the OECTs, yielding transistors with lower threshold voltages than PEDOT-based state-of-the-art devices and maximum transconductance voltage values close to 0 V, a key feature for the development of efficient organic bioelectronic devices. Subsequently, the azide moieties are employed to click alkyne-bearing molecules such as redox probes and biorecognition elements. The clicking of an alkyne-modified PEG4-biotin allows for the use of the avidin–biotin interactions to efficiently generate bioconstructs with proteins and enzymes. In addition, a dibenzocyclooctyne-modified thrombin-specific HD22 aptamer is clicked on the PEDOT-N3-OECTs, showing the application of the devices toward the development of organic transistors-based biosensors. Finally, the clicked OECTs preserve their electronic features after the different clicking procedures, demonstrating the stability and robustness of the fabricated transistors.

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Section: Resultsmentioning

confidence: 93%

“Clickable” Organic Electrochemical Transistors

Fenoy

Hasler

Quartinello³

et al. 2022

JACS Au

Self Cite

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“…Within this scenario, graphene-based field-effect transistors (gFETs) have arisen as a next-generation technology that combines high sensitivity and fast response with low-cost and easy-to-operate instrumentation. − Moreover, these devices have been shown to be an interesting choice for the real-time detection of molecules and biomarkers, while allowing miniaturization. ,− In addition, the use of gFETs for the study of the binding kinetics among biomolecules such as proteins, DNA, RNA, and ligands has also been evaluated. − However, there are still some obstacles that need to be overcome in order to transfer this technology to the market.…”

Section: Introductionmentioning

confidence: 99%

Using Graphene Field-Effect Transistors for Real-Time Monitoring of Dynamic Processes at Sensing Interfaces. Benchmarking Performance against Surface Plasmon Resonance

Scotto

Cantillo

Piccinini

et al. 2022

ACS Appl. Electron. Mater.

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Graphene field-effect transistors (gFETs) are promising tools for the development of precise and affordable techniques for the study of molecular binding kinetics, crucial in applications such as biomolecule therapies, drug discovery, and medical diagnostics. Nevertheless, determining the reliability and modeling the gFET signal for the monitoring of molecular binding and adsorption are still needed. Here, we prove that the gFET technology allows monitoring in real time the adsorption of both positive and negative polyelectrolytes, used as model charged macromolecules, using a low-cost portable gFET setup (Zaphyrus-W10), whose graphene channel was produced by reduction of graphene oxide. The gFET response is compared and validated against the surface plasmon resonance (SPR) technique. Remarkably, the electronic response is directly correlated with the mass adsorption, and very similar kinetic profiles are obtained for both techniques. Moreover, the adsorption kinetics of a polyelectrolyte assembled in a layer-by-layer give evidence that, even at ionic strengths near to the physiological conditions, the electrostatic interactions can be sensed at large distances from the graphene surface (20-fold higher in comparison to the solution Debye length). Biasing the gFET with a Ag/AgCl coplanar gate electrode avoids capacitive current contributions from nonbinding phenomena and displays a transistor signal proportional to the adsorbed mass. Furthermore, a marked amplification of the electronic signal without alteration of the macromolecule adsorption kinetics by using a Ag/AgCl gate in comparison with a nongated device is evidenced. Thus, the suitability of the coplanar-gated gFET technology for the study of molecular binding kinetics is illustrated.

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“…In addition, because of the limitation of purely electrical OFET sensors, the combined sensor platforms of FET and SPR have gained a lot of interest over the last years as they allow to measure not only the charge but also the mass of analytes, thus providing new insights into biological processes at solid interfaces [ 174 , 175 ].…”

Section: Applications Of the Flexible Ofets-based Sensorsmentioning

confidence: 99%

Flexible organic field-effect transistors-based biosensors: progress and perspectives

Zhang

et al. 2023

Anal Bioanal Chem

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Organic field-effect transistors (OFETs) have been proposed beyond three decades while becoming a research hotspot again in recent years because of the fast development of flexible electronics. Many novel flexible OFETs-based devices have been reported in these years. Among these devices, flexible OFETs-based sensors made great strides because of the extraordinary sensing capability of FET. Most of these flexible OFETs-based sensors were designed for biological applications due to the advantages of flexibility, reduced complexity, and lightweight. This paper reviews the materials, fabrications, and applications of flexible OFETs-based biosensors. Besides, the challenges and opportunities of the flexible OFETs-based biosensors are also discussed. Graphical abstract

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Field-Effect Transistor with a Plasmonic Fiber Optic Gate Electrode as a Multivariable Biosensor Device

Cited by 21 publications

References 43 publications

“Clickable” Organic Electrochemical Transistors

“Clickable” Organic Electrochemical Transistors

Using Graphene Field-Effect Transistors for Real-Time Monitoring of Dynamic Processes at Sensing Interfaces. Benchmarking Performance against Surface Plasmon Resonance

Flexible organic field-effect transistors-based biosensors: progress and perspectives

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