Fibronectin in tissue regeneration: timely disassembly of the scaffold is necessary to complete the build

Stoffels, Josephine M. J.; Zhao, Chao; Baron, Wia

doi:10.1007/s00018-013-1350-0

Cited by 74 publications

(75 citation statements)

References 137 publications

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“…FN promotes tissue regeneration in multiple ways. It is known to be involved in clearing the tissue debris at the site of injury and influences both cell proliferation and differentiation by serving as a scaffold for cell adhesion and migration (Stoffels et al, 2013). We observed that FN is expressed during zebrafish fin regeneration (Fig.…”

Section: Tnc Fn and Lam Show Distinct Expression Patterns During Finmentioning

confidence: 71%

Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration

Govindan

Iovine

2015

Gene Expression Patterns

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Extracellular matrix plays a dynamic role during the process of wound healing, embryogenesis and tissue regeneration. Caudal fin regeneration in zebrafish is an excellent model to study tissue and skeletal regeneration. We have analyzed the expression pattern of some of the well characterized ECM proteins during the process of caudal fin regeneration in zebrafish. Our results show that a transitional matrix analogous to the one formed during newt skeletal and heart muscle regeneration is synthesized during fin regeneration. Here we demonstrate that a provisional matrix rich in hyaluronic acid, tenascin C, and fibronectin is synthesized following amputation. Additionally, we observed that the link protein Hapln1a dependent ECM, consisting of Hapln1a, hyaluronan and proteoglycan aggrecan, is upregulated during fin regeneration. Laminin, the protein characteristic of differentiated tissues, showed only modest change in the expression pattern. Our findings on zebrafish fin regeneration implicates that changes in the extracellular milieu represent an evolutionarily conserved mechanism that proceeds during tissue regeneration, yet with distinct players depending on the type of tissue that is involved.

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Section: Tnc Fn and Lam Show Distinct Expression Patterns During Finmentioning

confidence: 71%

Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration

Govindan

Iovine

2015

Gene Expression Patterns

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“…Collagen IV, together with laminin, constitutes the basement membrane that surrounds blood vessels in vivo. In addition, fibronectin was abundantly detected in coculture systems and it is a known ECM component of developing microvessels, acting also as a scaffold for cell adhesion and migration 43,44 and playing a role in the elongation of microvessels. 45 All these ECM components were secreted by HDF, providing the support for capillary-like structures formation.…”

Section: Ecm Production By the Different Cellsmentioning

confidence: 99%

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Costa‐Almeida

Gómez-Lázaro

Ramalho

et al. 2015

Tissue Engineering Part A

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Cell-based approaches have emerged as a promising therapy to achieve successful vascularization in tissue engineering. Since fibroblasts activation and migration is required for physiological events relying on angiogenesis, we hypothesize herein that different fibroblasts exhibit distinct capacity to promote capillary-like structures assembly, by mature and progenitor endothelial cells (ECs). Outgrowth endothelial cells (OECs) were isolated from human umbilical cord blood samples and characterized by immunofluorescence and imaging flow cytometry for endothelial markers. Coculture systems were established using either human umbilical vein ECs (HUVECs) or OECs with fibroblasts, being evaluated at 7, 14, and 21 days of culture. Two types of human dermal fibroblasts (HDF) were used, namely neonatal human foreskin fibroblasts-1 (HFF-1) and juvenile HDF. OECs expressed EC markers and formed capillary-like structures. HFF-1 exhibited higher expression of transglutaminase-2, while HDF exhibited a higher expression of a-smooth muscle actin (a-SMA) and podoplanin, which were not observed for HFF-1. Formation of capillary-like structures was only observed in cocultures with HDF and not with HFF-1. No significant differences were found between HDF and OECs or HUVECs cocultures. These findings suggest that HDF is a preferential cell source for promoting vascularization, either using mature or progenitor ECs, probably due to their higher expression of a-SMA and podoplanin, and increased synthesis of extracellular matrix. This work opens new research possibilities regarding the use of specific fibroblast populations cocultured with ECs, as efficient partners for vascular development in regenerative medicine strategies.

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“…Currently, the interaction between collagen or fibronectin and cartilage regeneration has drawn much attention (Aigner and Stöve, 2003;Stoffels et al, 2013). However, few review papers about potential roles of laminin and its isoforms on cartilage-forming cells for cartilage regeneration are available.…”

Section: Introductionmentioning

confidence: 99%

The role of laminins in cartilaginous tissues: from development to regeneration

Sun¹,

Wang²,

Toh³

et al. 2017

eCM

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As a key molecule of extracellular matrix, laminin provides a delicate microenvironment for cell functions. Recent findings suggest that laminins expressed by cartilage-forming cells (chondrocytes, progenitor cells and stem cells) could promote chondrogenesis. However, few papers outline the effect of laminins on providing a favorable matrix microenvironment for cartilage regeneration. In this review, we delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. We also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration and survival. Furthermore, we scrutinized the potential influence of various laminin isoforms on cartilage-forming cells' proliferation and chondrogenic differentiation. With this information, we hope to facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration.

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Fibronectin in tissue regeneration: timely disassembly of the scaffold is necessary to complete the build

Cited by 74 publications

References 137 publications

Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration

Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

The role of laminins in cartilaginous tissues: from development to regeneration

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