Fibronectin in Experimental Granulation Tissue

Abstract

in experimental granulation tissue. Acta path. microbiol. immunol. scand. Sect. A. 90: 159-165. 1982.The temporal appearance of fibronectin in experimental granulation tissue has been studied using the immunoperoxidase technique on material fixed in formaldehyde, embedded in paraffin and pretreated with pepsin. Furthermore, the relationship between the distribution of fibronectin and connective tissue fibres, demonstrated as either argyrophilic or red by the van Gieson method, has been investigated. Fibronectin was demonstrated as part of the fibrinous network in the sponge before the formation of granulation tissue. In the development of granulation tissue fibronectin was Seen in the initial cellular stage codistributed with the newly formed fibres, first the argyrophilic fibres and later with those stained red by the van Gieson method, too. With maturation of the granulation tissue, predominated by these fibres, the amount of fibronectin was gradually reduced. The presence of fibronectin in the sponge already before the formation of granulation tissue suggests that fibronectin in the initial stage of inflammation derives from plasma. The increase in amount of fibronectin corresponding to the cellular (fibroblastic) proliferation and the formation of fibres, both argyrophilic and van Gieson positive, indicates that fibronectin in young granulation tissue is synthesized by fibroblastic cells. The relationship between the morphologically demonstrated argyrophilic fibres and van Gieson positive fibres and the biochemical type 111 and type I collagens is discussed.

Fibronectin in tears following surgical trauma to the eye

“…It seems reasonable to assume that the first peak represents a 'spill-over' from plasma, which contains about 400 mg/l (Eriksen et al 1982). The second peak might represent a FN production by fibroblasts in the corneoscleral cicatrix, which would be in agreement with the findings of Holund et al (1982), who observed a maximum staining intensity for FN in experimental granulation tissue from day 6-10.…”

Preparation and evaluation of wound healing activity of phytosomes of crocetin from Nyctanthes arbor-tristis in rats

“…The higher collagen content of wounds as assessed by the Haematoxylin and Eosin stain was better highlighted upon staining with Van Gieson. 28 Staining with Van Geison stain indicates staining of newly deposited collagen fibers in blue/green color. In addition, the alignment of the collagen fibers was also much better in wounds treated with the F1 and F2, also these were compact and parallel to the surface of the skin.…”

The influence of anti-fibronectin antibodies on interactions involving extracellular matrix components and cells: a possible pathogenic mechanism

“…Thus, functional weakness, reduced tensile strength and loss of tissue integrity may be a consequence of anti-Fn antibodies, and the anti-Fn antibodies may inhibit Fn from serving as a protein scaffold to direct tissue repair following inflammation. Although Fn is known to have an important role in wound healing, its role in parenchymal tissue injury is obscure [20], Anti-Fn antibodies have been shown to cause in vivo pathology using laboratory animals. Rabbits and mice immunized with Fn preparations developed glomerular injuries [21,22], and rats injected with anti-Fn antibodies developed glomerular injuries [23], Our preliminary results indicate an association between the occurrence of anti-Fn antibodies and active musculoskeletal involvement in SLE (manuscript in preparation).…”