Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time

Dalby, Matthew J.; Childs, Sarah J.; Riehle, Mathis O.; Johnstone, Heather; Affrossman, S.; Curtis, Adam

doi:10.1016/s0142-9612(02)00427-1

Cited by 242 publications

(170 citation statements)

References 26 publications

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“…Previous results published by Dalby et al (2002aDalby et al ( ,b, 2004a and show that several cell types react to growth on nanotopographical surfaces by changes in gene expression, motility, cytoskeletal organization, tyrosine kinase activity and other features in manner suggesting that there is some type of transduction of the signal that the surface has topographic features. The type of reaction is related both to the shape, spacing and actual dimensions of the features (Curtis et al 2001(Curtis et al , 2004Dalby 2004a-c, Dalby et al 2005) so that it is clear that several different types of signal can be discriminated.…”

Section: Discussionmentioning

confidence: 90%

Nanoprinting onto cells

Curtis

Dalby

Gadegaard

2005

J. R. Soc. Interface.

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Growing cells on surfaces bearing nanotopography signals makes many changes in cell gene expression and downstream changes in phenotype but the mechanisms for this have, so far, been obscure. We consider the question of whether the topography directly nanoimprints onto the cell as a component of the signal transduction system. Evidence we present from SEM, TEM and fluorescence detection of the arrangements of cytoskeletal components is consistent with the possibility that cells are nanoimprinted by the substrate. The nanoprinting does not interfere with integrin-mediated adhesion processes and may perhaps work through them. Time-lapse video studies of cells moving from areas bearing nanotopography to flat areas and vice versa suggests that the nanoimprinting takes 1-6 h to appear on the cell and a similar time to disappear when the cell moves from a flat surface to a nanotopographic one and back. This nanoprinting of cells would appear to be a novel type of cell signalling.

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Section: Discussionmentioning

confidence: 90%

Nanoprinting onto cells

Curtis

Dalby

Gadegaard

2005

J. R. Soc. Interface.

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“…Synthetic materials, such as PLGA, may create a biodegradable scaffold with the topography to introduce contact guidance to the cells in order for them to grow parallel to each other in one direction (Bian and Bursac, 2009;Choi et al, 2008;Lam et al, 2009). Contact guidance is the reaction of cells to topographical features (Dalby et al, 2003). Berendse et al (2003) and Peckham (2008) report that parallel f-actin cytoskeletal arrangement is important for successful sarcomeric formation (Berendse et al, 2003;Peckham, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In order to contract as a syncytium, muscle fibres must grow parallel to one another with identical anisotropy. This can be done by using a scaffold with the relevant topography to induce this behaviour via contact guidance (Bashur et al, 2006;Dalby et al, 2003). It is known that the structural components of the extracellular matrix (ECM) and their interaction with transmembrane proteins, such as integrins play a role in the organisation of tissues, including the organisation of myoblasts (Bray et al, 2008;Schiaffino and Partridge, 2008).…”

Section: Introductionmentioning

confidence: 99%

Aligned electrospun polymer fibres for skeletal muscle regeneration

Je²,

Downes³

2010

eCM

216

167

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Skeletal muscle repair is often overlooked in surgical procedures and in serious burn victims. Creating a tissueengineered skeletal muscle would not only provide a grafting material for these clinical situations, but could also be used as a valuable true-to-life research tool into diseases affecting muscle tissue. Electrospinning of the elastomer PLGA produced aligned fibres that had the correct topology to provide contact guidance for myoblast elongation and alignment. In addition, the electrospun scaffold required no surface modifications or incorporation of biologic material for adhesion, elongation, and differentiation of C2C12 murine myoblasts.

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“…The physicochemical properties of different materials could modulate cell morphology and behavior. Signaling from polymers that drive the cell growth pattern is complex and may originate from dipole and electric charge interaction forces, hydrogen bonds, electrostatic forces, hydrophilicity/hydrophobicity or surface free energy, and roughness and rigidity, surface tension, and substrate topography (18)(19)(20). The morphology of Vero cells on pure PLLA scaffolds observed here was similar to previous results obtained by our group (7,8,21), which showed that round or spreading cells attached on PLLA surface by thin cytoplasmatic filaments.…”

Section: Discussionmentioning

confidence: 99%

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

Santos

Ferreira

Duek

et al. 2005

Braz J Med Biol Res

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Vero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37°C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, 40/60, and 0/100). The present study evaluated morphology of cells grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis, and stained with Toluidine blue, pH 4.0, and Xylidine ponceau, pH 2.5. Our results showed that cell adhesion was better when 60/40 and 50/50 blends were used although cells were able to grow and proliferate on all blends tested. When using PLLA/PHBV (50/50) slightly flattened cells were observed on porous and smooth areas. PLLA/PHBV (40/60) blends presented flattened cells on smooth areas. PLLA/PHBV (0/100), which presented no pores, also supported spreading cells interconnected by thin filaments. Histological sections showed that cells grew as a confluent monolayer on different substrates. Cytochemical analysis showed basophilic cells, indicating a large amount of RNA and proteins. Hence, we detected changes in cell morphology induced by alterations in blend proportions. This suggests that the cells changed their differentiation pattern when on various PLLA/PHBV blend surfaces. Correspondence

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Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time

Cited by 242 publications

References 26 publications

Nanoprinting onto cells

Nanoprinting onto cells

Aligned electrospun polymer fibres for skeletal muscle regeneration

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

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