Fibroblast adhesion and activation onto micro‐machined titanium surfaces

Guillem-Marti, Jordi; Delgado, Luis M.; Godoy-Gallardo, María; Pegueroles, Marta; Herrero, Marta; Gil, F.J.

doi:10.1111/j.1600-0501.2012.02451.x

Cited by 51 publications

(41 citation statements)

References 43 publications

(64 reference statements)

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“…It was reported that focal adhesion molecules such as focal adhesion kinase (33)(34)(35), vinculin (28), and integrin (36,37) have roles in gingival fibroblast attachment on an abutment surface, but the underlying mechanisms are unclear. Therefore, we used an MTS assay to evaluate cell attachment on specimen surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, collagen type 1 (Col(I)-α1) was evaluated to examine extracellular matrix (ECM) and collagen formation (28). The goals of the present study were 1) to develop a method for removing contaminants on CAD/CAM abutments after milling and possible manual modifications in the laboratory, and 2) to determine the effects of decontamination on HGF attachment and bioactivity.…”

Section: Originalmentioning

confidence: 99%

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Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

et al. 2017

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The aim of this study was to develop an effective method for cleaning implant abutments made by computer-aided design and computer-aided manufacturing techniques and to investigate the effect of decontamination in vitro. Briefly, a newly developed reagent (PK) and/or vacuum plasma (Plasma) were used to clean the surfaces of zirconia disks, and the effects of this decontamination were evaluated by X-ray photoelectron spectroscopy. Human gingival fibroblasts (HGFs) were cultured on sample disks for 6, 24, and 48 h. We evaluated cell attachment and gene expression of the acute inflammatory cytokines interleukin-6 and vascular endothelial growth factor A, and type 1 collagen. In the PK and PK+Plasma groups, surface contaminants were reduced by washing. In addition, HGF attachments was increased in the PK and PK+Plasma groups. Gene expressions of interleukin-6 and vascular endothelial growth factor A were lower at 6 h. Gene expression of type 1 collagen was increased at all time points after seeding. These results suggest that decontamination of implant abutment surfaces is important in initial HGF attachment and may improve the biological seal of peri-implant soft tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Originalmentioning

confidence: 99%

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

et al. 2017

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“…Expression of adhesion (ICAM‐1, VCAM‐1), vascularization (VEGFA, VEGFR‐1, VEGFR‐2), pro‐thrombogenic (vWF, PAI‐1) and anti‐thrombogenic (tPA, eNOS) genes (Table ) in HUVECs on modified CoCr surfaces was determined through RT‐qPCR assay after 24, 48, and 72 h as previously described …”

Section: Methodsmentioning

confidence: 99%

Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis

Castellanos

Guillem-Marti

Mas‐Moruno

et al. 2017

J Biomedical Materials Res

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Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT‐qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM‐1 and VCAM‐1), vascularization (VEGFA, VEGFR‐1 and VEGFR‐2) and anti‐thrombogenicity (tPA and eNOS) were over‐expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro‐thrombogenic genes expression (PAI‐1 and vWF) decreased over time. Cell co‐cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 973–983, 2017.

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“…Formation of local inflammation by the introduction of a foreign body begins a cascade of events involved in wound healing. The direct cause of ineffective integration of biomaterials with the surrounding tissue can be the appearance of proteins on the surface of the implant, leading to the encapsulation implanted devices by connective tissue . Currently, the main challenge of tissue engineering is to understand the biology of the cells in contact with a biomaterial, as well as the development of an appropriate three‐dimensional architecture of scaffold serving cell adhesion, migration, growth, and proliferation .…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we tested human fibroblasts established cell line CCD‐39Lu. Fibroblasts have a key role in the first stage of the integration of implants with surrounding tissue . Examined materials were evaluated whether they exhibit a cytotoxic effect on used cell line.…”

Section: Discussionmentioning

confidence: 99%

In vitro biocompatibility of Ti‐45S5 bioglass nanocomposites and their scaffolds

Kaczmarek

Jurczyk

Rubiś

et al. 2013

J Biomedical Materials Res

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Titanium-10 wt % 45S5 Bioglass nanocomposites and their scaffolds were prepared by mechanical alloying (MA) followed by pressing, sintering, or combination of MA and a "space-holder" sintering process, respectively. An amorphous structure was obtained at 15 h of milling. The crystallization of the amorphous phase upon annealing led to the formation of a nanostructured Ti-10 wt % 45S5 Bioglass composite with a grain size of approximately 7 nm. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human fibroblasts CCD-39Lu was cultured in the presence of tested materials and its survival rate, and proliferation activity were examined. Furthermore, the influence of the Ti-45S5 Bioglass nanocomposites and microcrystalline titanium was tested on the growth of Candida albicans yeast. Biocompatibility tests carried out indicate that the nanocomposite Ti-10 wt % 45S5 Bioglass scaffolds could be a possible candidate for dental implants and other medicinal applications.

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Fibroblast adhesion and activation onto micro‐machined titanium surfaces

Cited by 51 publications

References 43 publications

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis

In vitro biocompatibility of Ti‐45S5 bioglass nanocomposites and their scaffolds

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