Fibrinogen receptor antagonists induce conformational changes of the human platelet glycoprotein IIb

Seyfarth, HJ; Koksch, Mario

doi:10.1002/cyto.b.20026

Cited by 4 publications

(2 citation statements)

References 62 publications

(57 reference statements)

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“…After two additional washing steps, streptavidin‐Cy5 (Jackson Immuno Research, Cambridgeshire, UK) was added and samples were incubated for 5 min, followed by another two washing steps and addition of 1 ml PBS prior to analysis. As a control for FRET analysis, platelets were also incubated with two monoclonal antibodies (clone SZ22 and P2, Beckman‐Coulter, Krefeld, Germany) directed against different epitopes of the GPIIb/IIIa, and analysis of FRET efficacy was performed as described earlier (7, 8).…”

Section: Methodsmentioning

confidence: 99%

High density lipoprotein modulates platelet function

Barlage

Boettcher

et al. 2006

Cytometry Pt A

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Background: Platelet activation by atherogenic lipoproteins can be antagonized by high density lipoprotein (HDL), probably via interaction with the ATP-binding cassette transporter A1 (ABCA1). Methods: ABCA1 expression and its association with cholesterol rich membrane domains was analyzed by mRNA and Western blot analysis. HDL effects on platelet receptor clustering were analyzed by flow cytometric analysis of fluorescence resonance energy transfer between fluorochrome-labeled antibodies. Results: ABCA1 expression increased upon megakaryocytic differentiation of human stem cells and ABCA1 pro-

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Section: Methodsmentioning

confidence: 99%

High density lipoprotein modulates platelet function

Barlage

Boettcher

et al. 2006

Cytometry Pt A

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“…Furthermore, flow cytometry seems to be applicable for the assessment of the amount of transfused platelets in patients with congenital deficiencies of platelet membrane proteins [21,22]. By using fluorescence resonance energy transfer, this method seems to be capable to investigate ligand induced conformational changes of glycoproteins or interactions with other cells [23,24], but these assays are only applicable in specialized laboratories. Furthermore, flow cytometry does not provide any information about the overall haemostasis.…”

Section: Flow Cytometrymentioning

confidence: 99%

Value of Platelet Function Testing in Monitoring Platelet Substitution

Meyer

Kiesewetter

Salama

2007

Transfus Med Hemother

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The use of platelet transfusion in the prevention and treatment of bleeding complications is still fiercely debated. To date, transfusions are usually monitored by measuring the post-transfusion platelet count and/or the corrected count increment. However, an increase in the platelet count does not necessarily correspond to an improvement in primary haemostasis. Although the response to platelet transfusions can be somewhat assessed by the physician treating the affected patient, the real effect remains frequently questionable. This review describes and discusses platelet function assays that might be used in the assessment of platelet transfusions.

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