2010
DOI: 10.1021/la102931a
|View full text |Cite
|
Sign up to set email alerts
|

Fibrinogen Adsorption on Mica Studied by AFM and in Situ Streaming Potential Measurements

Abstract: Adsorption of fibrinogen from aqueous solutions on mica was studied using AFM and in situ streaming potential measurements. In the first stage, bulk physicochemical properties of fibrinogen and the mica substrate were characterized for various ionic strength and pH. The zeta potential and number of uncompensated (electrokinetic) charges on the protein surfaces were determined from microelectrophoretic measurements. Analogously, using streaming potential measurements, the electrokinetic charge density of mica w… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

13
144
0

Year Published

2012
2012
2022
2022

Publication Types

Select...
7
1

Relationship

2
6

Authors

Journals

citations
Cited by 110 publications
(157 citation statements)
references
References 39 publications
13
144
0
Order By: Relevance
“…One reason for this is the amphoteric properties of the protein, with domains of different net charge, even though the whole fibrinogen molecule is net negatively charged at pH 7.4. (Wasilewska & Adamczyk 2011). Thus, the adsorption of fibrinogen on PDMAEMA, and to some extent also on PSPMA, can be associated with attractive electrostatic interactions (though this does not explain why adsorption is higher on the nominally neutral PHEMA-co-PEG10MA than on the zwitterionic PSBMA).…”
Section: Fouling Assaysmentioning
confidence: 97%
“…One reason for this is the amphoteric properties of the protein, with domains of different net charge, even though the whole fibrinogen molecule is net negatively charged at pH 7.4. (Wasilewska & Adamczyk 2011). Thus, the adsorption of fibrinogen on PDMAEMA, and to some extent also on PSPMA, can be associated with attractive electrostatic interactions (though this does not explain why adsorption is higher on the nominally neutral PHEMA-co-PEG10MA than on the zwitterionic PSBMA).…”
Section: Fouling Assaysmentioning
confidence: 97%
“…Thus, for NaCl concentration of respectively (see Table 1). Knowing the mobility, one can calculate the electrokinetic charge per molecule Q c (expressed in Coulombs) from the Lorenz-Stokes relationship [11,34] 3…”
Section: Bulk Characteristics Of Hsa Mica and Latex Particlesmentioning
confidence: 99%
“…Knowing the zeta potential, one can calculate its electrokinetic (uncompensated) charge using the Gouy-Chapman relationship [11,34].…”
Section: Bulk Characteristics Of Hsa Mica and Latex Particlesmentioning
confidence: 99%
See 1 more Smart Citation
“…This is an interesting estimate because knowing N c one can predict the bulk electrophoretic mobility of KfrA molecules, which is impractical using conventional methods (micro-electrophoretic measurements). The mobility can be calculated form the formula using the Lorenz-Stokes relationship previously applied for fibrinogen [ 25 ] and HSA [ 27 ] …”
Section: Formation Of Kfra Monolayers On Latex Particlesmentioning
confidence: 99%