Fibrin glue for reanastomosis of the Fallopian tube in the rabbit: adhesions and fertility

Abstract

Fertility and adhesions were examined in 41 rabbits in three treatment groups following the construction of microsurgical anastomoses with fibrin glue and by conventional suturing techniques. The three types of treatment were: isthmic anastomoses without resection, ampullary anastomoses, isthmic anastomoses following resection of oviduct segments. There were no significant differences between the two methods of performing the anastomoses in any of the three groups with respect to number of ovulations, number of implantations, nidation index, and pregnancy rate. The extent of the formation of adhesions was also no different between the two types of anastomosis construction. From the functional point of view, fibrin glueing of the tubes can be considered as good as microsurgical suturing. Another study is planned to examine whether this is also true from the morphological viewpoint. It must be remarked that fibrin glueing does not appear to be suitable for anastomoses in which a lumen-adjusting technique is needed or for deep tubocornual or intramural anastomoses.

Sutureless and Reduced Suture Anastomosis of Hollow Vessels With Fibrin Glue: A Review

“…This concurs with the morphological studies comparing FG and sutured rabbit Fallopian tubes in other studies [31,81]. However, the authors concluded that FG anastomoses with differing luminal diameters (e.g., isthmic-ampullary) are best performed with an intraluminal stent to reduce the risk of stulas, adhesions, and stenosis resulting from poor stump approximation [168,170,171]. In a study comparing AFG, Tisseel (commercial), polyglycolic acid suture anastomoses and nonoperated rabbit oviducts, Weis-Fogh et al [86] found no adhesions in the 22 AFG anastomoses.…”

“…All anastomoses were patent, and scanning electron microscopy revealed abundant microvilli on the anastomotic cells. Gauwerky et al [168][169][170][171] also compared fertility following reduced suture FG, and sutured ampullary and isthmic anastomoses. Neither the FG nor the sutured anastomoses demonstrated signi cant group advantages regarding the number of ovulations, number of implantations, pregnancy rate, or nidation index ([live fetuses + dead fetuses/total number of corpora lutea] £ 100).…”

Regeneration of Uterine Horn Using Porcine Small Intestinal Submucosa Grafts in Rabbits

“…The literature well documents the superiority of tubal reconstruction versus ART or IVF; however, there is no therapy, surgical or otherwise, established presently to restore tube length [14][15][16][17][18][19][20][21].…”

Use of Fibrin Sealant for Reproductive Surgery: A Randomized Study in the Rabbit Model

“…In tubal anastomosis animal models, the use of fibrin sealant was demonstrated to be as effective as microsurgi cal suturing in terms of postoperative fertility rates and epithelial morphology [16,17]. However, the issue of postoperative adhesion formation was not adequately ad dressed in the above studies; there appears to be similar [17] or higher [16] adhesion formation on the fibrin gluecovered compared to microsurgically sutured anasto moses.…”

“…However, the issue of postoperative adhesion formation was not adequately ad dressed in the above studies; there appears to be similar [17] or higher [16] adhesion formation on the fibrin gluecovered compared to microsurgically sutured anasto moses. In one clinical study, Tulandi [ 18] reported no dif- Nidation index = Number of embryos divided by corpora lutea on ipsilateral ovary.…”