Fibrin Glue as a Delivery Vehicle for Autologous Urothelial Cell Transplantation Onto a Prefabricated Pouch

Abstract

We conclude that cultured urothelial cells can be successfully reimplanted onto a prefabricated capsule-pouch via fibrin glue, showing potential for urinary reconstructions.

Sealing of Polyester Prostheses with Autologous Fibrin Glue and Bone Marrow

“…Plateletrich plasma can be polymerized in the presence of thrombin and calcium chloride. 24,25 In several experimental studies, this matrix has been used as a vector for healing promoters, including urothelial cells for the urinary tract, 26 synovial cells for joints, 27 chondrocytes, 28 and bone marrow cells. 29 For vascular healing, fibrin glue has been used as a support for endothelialization in arterial prosthetic grafts.…”

Autologous urothelial cells transplantation onto a prefabricated capsular stent for tissue engineered ureteral reconstruction

“…(6) An implantable stent designed with no direct connection between the pelvis and the bladder could preserve the natural antireflux mechanism, which could minimize the risk of upper urinary tract infection or kidney damage caused by water induced pressure. The induction of connective tissue capsule has provided several benefits for tissue engineering applications [40,41]. And previous studies have shown that all essential extracellular matrix molecules of a basement membrane, such as laminin, fibronectin and associated collagen molecules exist in such a capsule of connective tissue [41,42].…”

“…The induction of connective tissue capsule has provided several benefits for tissue engineering applications [40,41]. And previous studies have shown that all essential extracellular matrix molecules of a basement membrane, such as laminin, fibronectin and associated collagen molecules exist in such a capsule of connective tissue [41,42]. All these molecules are necessary for cell-cell and cell-ECM communication which is important for original phenotype of native urothelial cells.…”

In vitro stratifiziertes Urothelium und seine Bedeutung für die rekonstruktive Urologie

“…Hier zu wer den die iso lier ten Zel len en zy ma tisch ver ein zelt und in ge eig ne ten Trä ger me dien, z. B. Zell kul tur me di um oder Fi brinkleber, sus pen diert [12,13].…”

“…Hier zu wer den die iso lier ten Zel len en zy ma tisch ver ein zelt und in ge eig ne ten Trä ger me dien, z. B. Zell kul tur me di um oder Fi brinkleber, sus pen diert [12,13].Mit dem lang fris ti gen Ziel der Konstruk ti on kom plet ter Er satz ge we be im Labor wer den azel lu lä re Trä ger ma te ria li en (ma tri ces oder scaf folds) mit in vi tro ex pandier ten, en zy ma tisch ver ein zel ten uro thelia len Zel len be sie delt. Bei die sen Trä gerstruk tu ren han delt es sich um na tür li che oder syn the ti sche, bio degra dier ba re Mate ria li en.…”