Sealing of Polyester Prostheses with Autologous Fibrin Glue and Bone Marrow

Cardon, Alain; Chakfé, Nabil; Thaveau, Fabien; Gagnon, Édith; Hartung, Olivier; Aillet, S.; Kerdiles, Y; Dion, Yves-Marie; Jg, Kretz; Doillon, Charles J.

doi:10.1007/s100169910102

Cited by 15 publications

(10 citation statements)

References 34 publications

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“…In experimental studies, the immediate thrombogenicity of the fibrin‐glue graft was less than that of preclotted grafts (18). In the 1990s, fibrin matrix collected from the patient's plasma was used as a fibrin coating in the prosthetic vascular graft (9,19). Recent reports demonstrated that the fibrin coating in small caliber vascular prostheses decreased acute platelet deposition and prevented early thrombosis significantly (1,2).…”

Section: Discussionmentioning

confidence: 99%

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Thrombin‐free Fibrin Coating on Small Caliber Vascular Prostheses Has High Antithrombogenicity in Rabbit Model

et al. 2005

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Fibrin coatings on prosthetic vascular graft, which are conventionally produced by fibrinogen and thrombin, are expected to improve antithrombogenicity and healing characteristics. Thrombin is one of the factors of blood coagulation cascade; however, it has a possibility to play a negative role in the graft antithrombogenicity. The purpose of this study was to evaluate the performance of our new grafts, thrombin-free fibrin-coated small caliber vascular prostheses. Knitted polyester fabric vascular prostheses 2 mm in internal diameter were coated with fibrin coating with thrombin (Graft I) or without thrombin (Graft II). Both grafts were implanted in bilateral common carotid arteries of 35 Japanese white rabbits, with Graft I in one side and Graft II in the contralateral side. Graft patency, histology, thrombin activity, and platelet deposition were compared between both grafts on postoperative days (PODs) 1, 3, 7, 10, 14, 30, and 60. Both grafts were patent without thrombus or stenosis at each end point (maximal period, POD 60). Macro- and microscopic findings revealed that no obvious difference was observed between both grafts. Before graft implantation, thrombin activities in Grafts I and II were 0.711 +/- 0.086 and 0.009 +/- 0.007 optical density at 405 nm, respectively. Thrombin activity of Graft II was significantly less than that of Graft I in every period after graft implantation, and platelet deposition of Graft II was significantly less than that of Graft I until POD 30. Thrombin-free fibrin-coated vascular prostheses have superior performance of antithrombogenicity to conventional fibrin-coated vascular prostheses with thrombin.

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Section: Discussionmentioning

confidence: 99%

“…1 FG with thrombin (Graft I) 2 FG without thrombin (Graft II) Graft I as a control was coated with fibrin glue as previously described (9). Briefly, 20 mL of blood from normal human donors was collected with 4 mL of acid-citrate-dextrose (ACD) anticoagulant solution.…”

Section: Preparation Of Vascular Prosthesesmentioning

confidence: 99%

Thrombin‐free Fibrin Coating on Small Caliber Vascular Prostheses Has High Antithrombogenicity in Rabbit Model

et al. 2005

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“…Endothelialization of a small‐diameter vascular graft is a prerequisite for its long‐term function, because ECs deter thrombogenesis and calcification. In order to improve endothelialization, synthetic prostheses have been coated with various compounds, such as collagen, heparin, fibrin (Fb), loaded with endothelial growth factor, fibroblast growth factor (FGF), seeded with ECs, bone marrow‐derived endothelial progenitor cells, and cells from venous or adipose tissues …”

Section: Introductionmentioning

confidence: 99%

Improved adhesion and differentiation of endothelial cells on surface‐attached fibrin structures containing extracellular matrix proteins

Filová

Brynda

Riedel

et al. 2013

J Biomedical Materials Res

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Currently used vascular prostheses are hydrophobic and do not allow endothelial cell (EC) adhesion and growth. The aim of this study was to prepare fibrin (Fb)-based two-dimensional (2D) and three-dimensional (3D) assemblies coated with extracellular matrix (ECM) proteins and to evaluate the EC adhesion, proliferation and differentiation on these assemblies in vitro. Coating of Fb with collagen, laminin (LM), and fibronectin (FN) was proved using the surface plasmon resonance technique. On all Fb assemblies, ECs reached higher cell densities than on polystyrene after 3 and 7 days of culture. Immunoflurescence staining showed better assembly of talin and vinculin into focal adhesion plaques, and also more apparent staining of vascular endothelial cadherin on surface-attached 3D Fb and protein-coated Fb assemblies. On these samples, ECs also contained a lower concentration of intercellular adhesion molecule-1, measured by enzyme-linked immunosorbent assay. Higher concentrations of CD31 (platelet-endothelial cell adhesion molecule-1) were found on 3D Fb coated with LM, and higher concentrations of von Willebrand factor were found on 3D Fb coated with type I collagen or LM in comparison to 2D Fb layers. The results indicate that ECM protein-coated 2D and 3D Fb assemblies can be used for versatile applications in various tissue replacements where endothelialization is desirable, for example, vascular prostheses and heart valves.

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“…Compared to the 10% group without glue, this difference was statistically significant (P Ͻ .003), suggesting that endoprosthetic fixation can be increased without excessive oversizing. Cardon et al 26 reported moderate intimal hyperplasia and intense thickening around the prosthesis in pigs 14 days after implantation of open aortobifemoral grafting impregnated with fibrin glue. The fibrin matrix acts as a pathway that facilitates the migration of inflammatory cells.…”

Section: Discussionmentioning

confidence: 98%

Biomechanical and histologic analysis in aortic endoprosthesis using fibrin glue

Almeida

Yoshida

Hafner

et al. 2011

Journal of Vascular Surgery

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Sealing of Polyester Prostheses with Autologous Fibrin Glue and Bone Marrow

Cited by 15 publications

References 34 publications

Thrombin‐free Fibrin Coating on Small Caliber Vascular Prostheses Has High Antithrombogenicity in Rabbit Model

Thrombin‐free Fibrin Coating on Small Caliber Vascular Prostheses Has High Antithrombogenicity in Rabbit Model

Improved adhesion and differentiation of endothelial cells on surface‐attached fibrin structures containing extracellular matrix proteins

Biomechanical and histologic analysis in aortic endoprosthesis using fibrin glue

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