Fibrin gels and their clinical and bioengineering applications

Janmey, Paul A.; Winer, Jessamine P.; Weisel, John W.

doi:10.1098/rsif.2008.0327

Cited by 571 publications

(479 citation statements)

References 95 publications

Supporting

Mentioning

461

Contrasting

Unclassified

Order By: Relevance

“…Fibrin gels occur widely in the human body and are important in haemostasis following injury (Janmey et al 2009). Fibrin gels form following the cleavage of fibrinogen by thrombin to expose regions on the fibrin molecules that interact allowing self assembly of protofibrils which aggregate and lengthen.…”

Section: Fibrinmentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

View full text Add to dashboard Cite

To cite this version:Nicola C. Hunt, Liam M. Grover. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.733-742. <10.1007/s10529-010-0221-0>. Abstract There has been a consistent increase in the mean life expectancy of the population of the developed world over the past century. Healthy life expectancy, however, has not increased concurrently. As a result we are living a larger proportion of our lives in poor health and there is a growing demand for the replacement of diseased and damaged tissues. While traditionally tissue grafts have functioned well for this purpose, the demand for tissue grafts now exceeds the supply. For this reason, research in regenerative medicine is rapidly expanding to cope with this new demand. There is now a trend towards supplying cells with a material in order to expedite the tissue healing process. Hydrogel encapsulation provides cells with a three dimensional environment similar to that experienced in vivo and therefore may allow the maintenance of normal cellular function in order to produce tissues similar to those found in the body. In this review we discuss biopolymeric gels that have been used for the encapsulation of mammalian cells for tissue engineering applications as well as a brief overview of cell encapsulation for therapeutic protein production. This review focuses on agarose, alginate, collagen, fibrin, hyaluronic acid and gelatin since they are widely used for cell encapsulation. The literature on the regeneration of cartilage, bone, ligament, tendon, skin, blood vessels and neural tissues using these materials has been summarised.

show abstract

Section: Fibrinmentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

View full text Add to dashboard Cite

show abstract

“…Additionally, many growth factors in the blood and various cell type integrin can interact and bind to several sites of the fibrin (11). These properties make fibrin an attractive matrix for cell migration and differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is not known whether a scaffold inside the canal space can improve tissue ingrowth compared to leaving the canal space empty. A simple scaffold would be a fibrin gel, which can simulate a blood clot (11). A blood clot can be hard to obtain clinically (3).…”

Section: Introductionmentioning

confidence: 99%

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

Ruangsawasdi

Zehnder

Weber

2014

Journal of Endodontics

View full text Add to dashboard Cite

INTRODUCTION In pulpless immature human premolars implanted in rodents, this study investigated whether fibrin gel offered advantages over leaving the root canal empty regarding soft tissue ingrowth and cell differentiation. METHODS Root canals of extracted human immature premolars (n = 12) were accessed and then irrigated with 5% sodium hypochlorite followed by 17% ethylenediaminetetraacetic acid. Root canals were then either left empty or filled with a fibrin gel (n = 6 each) before being placed subcutaneously on top of the calvarial bone of rats (1 tooth per rat) for 12 weeks. After sacrifice, teeth were histologically assessed. Tissue ingrowth was quantified and compared between groups using the Mann-Whitney U test (P < .05). Cells adhering to the pulp canal wall were immunohistochemically screened for the presence of bone sialoprotein (BSP) and dentin sialoprotein (DSP). RESULTS More tissue grew into the pulp space when teeth were filled with fibrin gel (P < .05). The presence of fibrin gel affected not only the extent of tissue ingrowth but also tissue morphology and differentiation of cells contacting the dentinal wall. In the fibrin gel group, newly formed tissue was similar to normal pulp, constituted of inner pulp, cell-rich zone, cell-free zone, and an apparent odontoblast layer, which stained positive for BSP and DSP. Newly formed blood vessels were also more abundant compared with the initially empty root canals. CONCLUSIONS Under the conditions of this study, fibrin gel improved cell infiltration and cell-dentin interaction. Both are necessary for pulp tissue regeneration.

show abstract

“…Fibrinogen is a rod-shaped complex glycoprotein with a molecular weight of 340 Da, 45 nm in length, contains three pairs of polypeptide chains Aa, Bb, and g, and all are held together by 29 disulfide bonds. 24,25 Whether alone or in combination both hyaluronan and fibrin have been extensively studied for their regenerative potential. 26,27 However, the majority of hydrogel applications have been either for direct bone ossification, cartilage, or osteochondral regeneration.…”

Section: Introductionmentioning

confidence: 99%

Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Mikael

Nukavarapu

2017

J Biomed Mater Res

View full text Add to dashboard Cite

Recapitulating long bone repair through endochondral ossification (EO) is increasingly becoming a more popular approach. A successful EO Process depends greatly on the establishment of a healthy hypertrophic-cartilage template (HCT). The aim of this work is to design a hydrogel system, which closely mimics the extracellular matrix of HCT. We examined the combinatorial effect of two commonly used hydrogels for bone and cartilage regeneration strategies, hyaluronan (HA) and fibrin (FB), to induce HCT formation. Hydrogel combinations were evaluated using a clinically relevant cell source, human bone marrow mesenchymal stem cells (hBMSCs). The results establish that with increasing HA (50-90%) the chondrogenic and its subsequent hypertrophy trend improved, with 70:30 HA:FB combination showing the highest and most uniform expression of chondrogenic and hypertrophic stage specific markers. This combination also showed superior support for cell micro-aggregation and differentiation. Thus, 70:30 HA-FB matrix demonstrated a healthy formation of chondrogenic and hypertrophic stages with rich stage-specific ECM components. This study demonstrates that with the appropriate hydrogel design it is possible to develop effective tissue engineering therapies for bone defect repair and regeneration through endochondral ossification by establishing a healthy HCT. V C 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B:Appl Biomater, 106B: 300-309, 2018.

show abstract

Fibrin gels and their clinical and bioengineering applications

Cited by 571 publications

References 95 publications

Cell encapsulation using biopolymer gels for regenerative medicine

Cell encapsulation using biopolymer gels for regenerative medicine

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

Hybrid extracellular matrix design for cartilage‐mediated bone regeneration

Contact Info

Product

Resources

About