Fibrin-Embedded Adipose Derived Stem Cells Enhance Skin Flap Survival

Reichenberger, M; Mueller, Wolf; Schäfer, Amelia; Heimer, Sina; Leimer, Uwe; Laß, Ulrike; Germann, Günter; Köllensperger, Eva

doi:10.1007/s12015-011-9341-8

Cited by 17 publications

(16 citation statements)

References 34 publications

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“…Cells at P4, which still possessed multipotent differentiation potential (Figure 2), were used for transplantation in this study. Compared with similar studies, the number of cells injected in this study was higher than others [8,[30][31][32][33]. Despite this, cells expanded at passage 6 could increase the number of cells for injection, but did not improve the protective effects in skin flap survival (data not shown).…”

Section: Discussioncontrasting

confidence: 78%

Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

Xu¹,

Yang²

2016

J Stem Cell Res Ther

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Section: Discussioncontrasting

confidence: 78%

Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

Xu¹,

Yang²

2016

J Stem Cell Res Ther

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“…However, similar studies by other researchers (1, 3, 5-7, 31, 32). In order to increase flap survival have done, this corresponds) as follows: group 1 received an AAM injection, group 2 underwent BM-MSC transplantation, group 3 received AAM injection + BM-MSC transplantation, and group 4 was the control group, receiving only saline.…”

Section: Methodssupporting

confidence: 80%

“…The effects of BM-MSCs and adipose mesenchymal stem cells (AD-MSCs) on skin flap survival have often been studied in dorsal random skin flaps in rats (5). In the majority of these studies, the length and area of necrotic tissue significantly decreased as a result of treatment with stem cells (3, 5-7, 33). …”

Section: Discussionmentioning

confidence: 99%

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Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats

Chehelcheraghi

Eimani

Homayoonsadraie

et al. 2016

Iran Red Crescent Med J

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BackgroundThe necrotic skin flap represents a great challenge in plastic and reconstructive surgery. In this study, we evaluated the effect of bioscaffolds, acellular amniotic membranes (AAMs), and bone marrow-derived mesenchymal stem cells (BM-MSCs) on random skin flap (RSF) survival in rats by applying a cell-free extracellular matrix scaffold as a supportive component for the growth and proliferation of BM-MSCs on RSFs. AAM matrix scaffolds were created by incubating AMs in ethylenediaminetetraacetic acid 0.05% at 37°C, and cell scrapers were used.ObjectivesThe aim of the present study was to assess the effect of AAM as a scaffold in TE, and combined with transplanted BM-MSCs, on the survival of RSFs and on the biomechanical parameters of the incision-wound flap margins 7 days after flap elevation.Materials and MethodsBM-MSCs and AAMs were transplanted into subcutaneous tissue in the flap area. On the 7th postoperative day, the surviving flap areas were measured using digital imaging software, and the flap tissue was collected for evaluation. Forty rats were randomly divided into four groups of 10 each: group 1 received an AAM injection; group 2 underwent BM-MSC transplantation; group 3 received both AAM injection + BM-MSC transplantation; and group 4 was the control group, receiving only saline.ResultsThe survival area in the AAM/BM-MSC group was significantly higher than in the control group (18.49 ± 1.58 versus 7.51 ± 2.42, P < 0.05). The biomechanical assessment showed no significant differences between the experimental groups and the control group (P > 0.05), and there was no correlation with flap survival.ConclusionsOur findings showed that the treatment of flaps with BM-MSC and AAM transplantations significantly promoted flap survival compared to a control group. The viability of the flap was improved by combining BM-MSCs with AAM matrix scaffolds.

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“…Hemodynamic abnormalities in pulmonary arterial hypertension may be reversed using ASCs-a finding thought to be attributable to their induction of angiogenesis and increased formation of small, ISRN Stem Cells pulmonary arteries [304]. Increased capillary densities and higher blood flow have been reported in several studies using ASCs for the healing of skin wounds and improvement of skin flap survival [305,306]. At the cosmetic level, adipose grafts, transplanted with ASCs survive at higher levels, are 35% larger and show increased neoangiogenesis when compared to grafts transplanted without these stem cells [224,225].…”

Section: Asc-directed Tissue Regeneration: the Role Of Vascularizationmentioning

confidence: 99%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. As of today, thousands of research and clinical articles have been published using ASCs, describing their possible pluripotency in vitro, their uses in regenerative animal models, and their application to the clinic. This paper outlines the progress made in the ASC field since their initial description in 2001, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo, their use in mediating inflammation and vascularization during tissue regeneration, and their potential for reprogramming into induced pluripotent cells.

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Fibrin-Embedded Adipose Derived Stem Cells Enhance Skin Flap Survival

Cited by 17 publications

References 34 publications

Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

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