Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats

Chehelcheraghi, Farzaneh; Eimani, Hussein; Homayoonsadraie, Seyed; Torkaman, Giti; Amini, Abdollah; Majd, Hamid Alavi; Shemshadi, Hashem

doi:10.5812/ircmj.25588

Cited by 25 publications

(26 citation statements)

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“…The observed limited cell growth on the surface of native amnion led us, like others [28] A variety of techniques have been used to decellularize the amnion [15], [24], [26], [28]. We optimized a trypsin-EDTA, nuclease decellularization protocol that eliminated cell and nuclear materials from the amnion.…”

Section: Discussionmentioning

confidence: 99%

“…glycosamnioglycans [17], [22], [23]. Although in most studies the amnion have been used without much modification other than dehydration, several investigations have decellularized it beforehand using a range of approaches including freeze-thawing [24], [25], EDTA [26], and trypsin-EDTA treatment [12], [13].…”

Section: Introductionmentioning

confidence: 99%

“…Long-term amnion preservation is essential for tissue engineering. While matrix preservation was not considered or attempted in many studies [24]- [26], the beneficial effects of preservation were demonstrated by others [28]. Reported preservation work utilized different methods including dehydration [13], [28], refrigeration or freezing [12].…”

Section: Introductionmentioning

confidence: 99%

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Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Swim

Albertario

Iacobazzi

et al. 2019

Tissue Engineering Part A

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Current vascular replacement grafts used in congenital heart defect corrective surgery have poor longevity and growth potential. Recipient patients often require multiple reoperations. Tissue engineering has the promise to produce a graft with the potential to grow, remodel and repair. Here we aimed at developing an amnion-based scaffold suitable for cardiovascular tissue engineering applications and in vivo usage. The developed human amnion-based scaffold was made by an enzymatic decellularization process followed by freeze-drying as a single or multi-layered structure. These structures were compared to native amnion for seeded cell viability and biomechanical properties then tested for in vivo biocompatibility. Our results demonstrated that while native amnion tissue supported little cell growth, the decellularized-amnion allowed cell engraftment and proliferation cell survival. Additionally, preservation of the scaffold by freeze-drying as a single layer, allowed further improved cell engraftment and cell growth. Multi-layering the freeze-dried amnion-scaffolds resulted in a similar cell growth potential of the single layered construct but superior mechanical strength. The multi-layered construct showed in vitro biocompatibility with endothelial cells, smooth muscle cells, cardiac myocytes, and thymus and cord-blood-derived MSCs. When implanted in a piglet model of left pulmonary artery grafting, the multi-layered construct showed its in vivo suitability and biocompatibility for vascular repair as demonstrated by the development of newly formed endothelium in the intima, a smooth muscle cell-rich medial layer and an adventitia containing new vasa vasorum. endothelial cell layer in the inner side of the graft and a smooth muscle layer in the outer side. In conclusion, our developed amnion-derived scaffold represents an off-the-shelf biocompatible structure that can be seeded with the patient's own MSCs to produce an autologous vascular graft.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Swim

Albertario

Iacobazzi

et al. 2019

Tissue Engineering Part A

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“…Recently, MSCs have been used to enhance the survival of ischemic RSFs. 15,23,[29][30][31][32] In addition to their F I G U R E 4 Mean ± standard error of survival of flap at day 7 after surgery of the study groups, compared by one way analysis of variance (ANOVA) and least significant difference (LSD) tests F I G U R E 5 Mean ± standard error of stereological parameters at day 7 after surgery of the study groups, compared by ANOVA and LSD tests. ANOVA, analysis of variance; LSD, least significant difference F I G U R E 6 Mean ± standard error of number of vascular endothelial growth factor (VEGF) + cells at day 7 after surgery of the study groups, compared by ANOVA and LSD tests.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells improve survival in ischemic diabetic random skin flap via increased angiogenesis and VEGF expression

Chehelcheraghi

Chien

Bayat

2019

J of Cellular Biochemistry

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Random skin flaps (RSFs) are cutaneous flaps. Despite the negative impact of diabetes mellitus (DM) on RSF viability, they are commonly used in diabetic patients. In this study, we have assessed bone marrow mesenchymal stem cell (BMMSC) treatment on RSF survival, tensiometrical parameters, angiogenesis, and mast cells (MCs) count in an ischemic RSF model in rats with type 1 DM (T1DM). We induced T1DM in 30 Wistar adult male rats. The animals were assigned to three groups of 10 rats per group as follows: group 1 (control); group 2 (placebo), and group 3 (BMMSCs). A 30 × 80 mm RSF was created in each rat. On day 7, we measured the viable portion of each RSF. A sample was taken for histological and immunohistochemistry studies, fibroblasts, MCs, angiogenesis, collagen bundle density, and the presence of vascular endothelial growth factor (VEGF)+ cells. An additional sample was taken to evaluate the flap's incision strength. Treatment with BMMSCs (17.8 ± 0.37) significantly increased RSF survival compared with the control (13.3 ± 0.35) and placebo (16.1 ± 0.27) groups (one‐way analysis of variance, P = .000; least significant difference, P = .000, P = .002). There was a significant improvement in angiogenesis, as confirmed by stereologic examination. Assessment of VEGF+ cells showed prominent neovascularization in BMMSC‐treated RSFs compared with the control and placebo groups. Subdermal injection of BMMSC significantly increased ischemic RSF survival as a result of stimulated neovascularization in T1DM rats. Treatment of diabetic RSF with BMMSCs showed no beneficial effects in the fibroblast number and biomechanical parameters for the repair of ischemic wounds in the rat model. Treatment with BMMSCs significantly increased collagen bundle density.

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“…5 Wound healing is a complicated process that involves clot formation, inflammation, granulation, epithelialization, and remodeling. 7,8 Studies on wound healing have investigated the effect of oxygen therapy, [9][10][11] biomechanics, 12 radiotherapy and medical pomades. 13 Low-level laser therapy (LLLT) is a good modality for wound healing.…”

Section: Introductionmentioning

confidence: 99%

Combined Effect of Low-Level Laser Treatment and Levothyroxine on Wound Healing in Rats With Hypothyroidism

et al. 2018

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Please cite this article as follows: Firouzi A, Norozian M, Amini A, Abdollahifar MA, Abbaszadeh HA, Fadaei Fathabadi F. Combined effect of low-level laser treatment and levothyroxine on wound healing in rats with hypothyroidism. AbstractIntroduction: Hypothyroidism delays wound healing by reducing the synthesis of keratinocytes, fibroblast cells, and collagen. Methods for enhancement of wound healing include laser therapy and hormone therapy. The current study evaluated the combined effect of laser and levothyroxine therapy to cure wounds in male rats with hypothyroidism. Methods: Sixty male Wistar rats were randomly divided into 5 groups: (1) healthy controls;(2) controls with hypothyroidism; (3) hypothyroidism + laser treatment; (4) hypothyroidism + levothyroxine treatment; (5) hypothyroidism + laser + levothyroxine treatment. Hypothyroidism was induced by dissolving 4 mg of methimazole in 100 mL of drinking water daily for 28 days. After hypothyroidism had been confirmed, a longitudinal incisional wound was created on the dorsal rib cages of the rats. The wounds that received laser treatment were divided into 12 sections and treated at 810 nm wavelength and 0.2 J/cm 2 of energy density for 200 seconds. Levothyroxine was administrated in doses of 20 µg/kg/d i.p. All groups were divided into 3 subgroups for testing on days 4, 7 and 14. Samples were collected in all the subgroups. Results: The results showed that hypothyroidism reduced fibrous tissue volume, fibroblasts, and basal cell numbers. The combined effect of laser and levothyroxine improved all parameters. Conclusion: Combined laser and levothyroxine treatment showed the best effect on wound healing and accelerated the closure of the wounds.

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Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats

Cited by 25 publications

References 50 publications

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Mesenchymal stem cells improve survival in ischemic diabetic random skin flap via increased angiogenesis and VEGF expression

Combined Effect of Low-Level Laser Treatment and Levothyroxine on Wound Healing in Rats With Hypothyroidism

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