Fibrin deposition associates with cartilage degeneration in arthritis

Hügle, Thomas; Nasi, Sonia; Ehirchiou, Driss; Omoumi, Patrick; So, Alexander; Busso, Nathalie

doi:10.1016/j.ebiom.2022.104081

Cited by 10 publications

(8 citation statements)

References 61 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once DAMPs are bound to PRRs, a signaling cascade, ultimately, causes the activation of crucial regulator factors for the inflammatory response, one of them being the nuclear factor-kB (NF-kB) [ 51 ]. As a consequence, several inflammatory mediators were produced: cytokines such as the interleukin (IL)-1b and IL-6 or the tumor necrosis factor (TNF)-a, cathepsins, chemokines, and catabolic factors, such as matrix metalloproteinase (MMP), as well as the complement cascade [ 52 ] ( Figure 1 ).…”

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

“…Intracellular DAMPs are represented by different molecules with immunogenic properties produced by disrupting apoptotic or necrotic cells [ 48 ]. An association has been found between fibrin deposits and the severity of cartilage damage [ 52 ]. The articular damage is exerted through the upregulation of Adamts5 and MMP-13 in chondrocytes and the development of chondro-synovial adhesion, which specifically occurred in fibrin-rich cartilage areas [ 52 ].…”

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

“…An association has been found between fibrin deposits and the severity of cartilage damage [ 52 ]. The articular damage is exerted through the upregulation of Adamts5 and MMP-13 in chondrocytes and the development of chondro-synovial adhesion, which specifically occurred in fibrin-rich cartilage areas [ 52 ]. Fibrin deposits were also found to stimulate the production of inflammatory cytokines by macrophages, via the TLR-4 pathway [ 48 ].…”

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

See 2 more Smart Citations

Knee Osteoarthritis: Epidemiology, Pathogenesis, and Mesenchymal Stem Cells: What Else Is New? An Update

Giorgino

Albano

Fusco

et al. 2023

IJMS

View full text Add to dashboard Cite

Osteoarthritis (OA) is a chronic disease and the most common orthopedic disorder. A vast majority of the social OA burden is related to hips and knees. The prevalence of knee OA varied across studies and such differences are reflected by the heterogeneity of data reported by studies conducted worldwide. A complete understanding of the pathogenetic mechanisms underlying this pathology is essential. The OA inflammatory process starts in the synovial membrane with the activation of the immune system, involving both humoral and cellular mediators. A crucial role in this process is played by the so-called “damage-associated molecular patterns” (DAMPs). Mesenchymal stem cells (MSCs) may be a promising option among all possible therapeutic options. However, many issues are still debated, such as the best cell source, their nature, and the right amount. Further studies are needed to clarify the remaining doubts. This review provides an overview of the most recent and relevant data on the molecular mechanism of cartilage damage in knee OA, including current therapeutic approaches in regenerative medicine.

show abstract

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

Section: The Oa Inflammatory Response: Mechanism and Mediatorsmentioning

confidence: 99%

See 1 more Smart Citation

Knee Osteoarthritis: Epidemiology, Pathogenesis, and Mesenchymal Stem Cells: What Else Is New? An Update

Giorgino

Albano

Fusco

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Ribera et al [ 24 ] first demonstrated significantly increased levels of synovial D-dimer within the septic joints of foals. Other studies have supported that inflamed synovium secretes large amounts of fibrin, ultimately resulting in increased intra-articular concentrations of D-dimer which can efflux out of the joint and into circulation[ 25 ].…”

Section: D-dimermentioning

confidence: 99%

Utility of D-dimer in total joint arthroplasty

Cutter¹,

Lum²,

Giordani³

et al. 2023

World J Orthop

View full text Add to dashboard Cite

As the number of patients receiving total joint replacements continues to rise, considerable attention has been directed towards the early detection and prevention of postoperative complications. While D-dimer has long been studied as a diagnostic tool in venous thromboembolism (VTE), this assay has recently received considerable attention in the diagnosis of periprosthetic joint infection (PJI). D-dimer values are substantially elevated in the acute postoperative period after total joint arthroplasty, with levels often exceeding the standard institutional cutoff for VTE (500 µg/L). The utility of D-dimer in detecting VTE after total joint replacement is currently limited, and more research to assess its value in the setting of contemporary prophylaxis protocols is warranted. Recent literature supports D-dimer as a good to excellent biomarker for the diagnosis of chronic PJI, especially when using serum sample technique. Providers should exercise caution when interpreting D-dimer levels in patients with inflammatory and hypercoagulability disorders, as the diagnostic value is decreased. The updated 2018 Musculoskeletal Infection Society criteria, which includes D-dimer levels > 860 µg/L as a minor criterion, may be the most accurate for diagnosing chronic PJI to date. Larger prospective trials with transparent lab testing protocols are needed to establish best assay practices and optimal cutoff values for D-dimer in the diagnosis of PJI. This review summarizes the most current literature on the value of D-dimer in total joint arthroplasty and elucidates areas for future progress.

show abstract

“…[1][2][3][4][5] Fibrinogen is a multifunctional plasma protein with coagulation effect, which is not only involved in a variety of physiological and pathological processes, but also has a close relationship with malignant tumors. [6][7][8] Under the proteolytic cleavage of thrombin, fibrinogen can produce fibrin with a huge spatial structure that is insoluble in water. 9 It has been shown that fibrin deposition occurs in the tumor interstitium and differs significantly from normal tissue.…”

Section: Introductionmentioning

confidence: 99%

A two‐step electrochemical biosensor based on Tetrazyme for the detection of fibrin

Jiang,

Wang,

Luo

et al. 2023

Biotech and App Biochem

View full text Add to dashboard Cite

In this study, an electrochemical biosensor was constructed for the detection of fibrin, specifically by a simple two‐step approach, with a novel artificial enzyme (Tetrazyme) based on the DNA tetrahedral framework as signal probe. The multichannel screen‐printed electrode with the activated surface cannot only remove some biological impurities, but also serve as a carrier to immobilize a large number of antigen proteins. The DNA tetrahedral nanostructure was employed to ensure the high sensitivity of the probe for biological analysis. The hemin was chimeric into the G‐quadruplex to constitute the complex with peroxidase catalytic activity (hemin/G4‐DNAzyme), subsequently, Tetrazyme was formed through combining of this complex and DNA tetrahedral nucleic acid framework. The artificial enzyme signal probe formed by the covalent combination of the homing peptide (Cys‐Arg‐Glu‐Lys‐Ala, CREKA), which is the aptamer of fibrin and the new artificial enzyme is fixed on the surface of the multichannel carbon electrode by CREKA‐specific recognition, so as to realize the sensitive detection of fibrin. The feasibility of sensing platform was validated by cyclic voltammetry (CV) and amperometric i–t curve (IT) methods. Effects of Tetrazyme concentration, CREKA concentrations and hybridization time on the sensor were explored. Under the best optimal conditions of 0.6 μmol/L Tetrazyme, 80 μmol/L CREKA, and 2.5 h reaction time, the immunosensor had two linear detection ranges, 10–40 nmol/L, with linear regression equation Y = 0.01487X − 0.011 (R2 = 0.992), and 50–100 nmol/L, with linear regression equation Y = 0.00137X + 0.6405 (R2 = 0.998), the detection limit was 9.4 nmol/L, S/N ≥ 3. The biosensor could provide a new method with great potential for the detection of fibrin with good selectivity, stability, and reproducibility.

show abstract

Fibrin deposition associates with cartilage degeneration in arthritis

Cited by 10 publications

References 61 publications

Knee Osteoarthritis: Epidemiology, Pathogenesis, and Mesenchymal Stem Cells: What Else Is New? An Update

Knee Osteoarthritis: Epidemiology, Pathogenesis, and Mesenchymal Stem Cells: What Else Is New? An Update

Utility of D-dimer in total joint arthroplasty

A two‐step electrochemical biosensor based on Tetrazyme for the detection of fibrin

Contact Info

Product

Resources

About