Fibrin-Based Drug Delivery Systems. II. The Preparation and Characterization of Microbeads

Ho, Hsio O.; Hsiao, Chih Chuan; Chen, Chau Yang; Sokoloski, Theodore D.; Sheu, Ming Thau

doi:10.3109/03639049409038316

Cited by 8 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Introduction of additional crosslinks in the fibrin gels with crosslinking agents like glutaraldehyde lowers pore size as well as susceptibility to proteolysis [33,66,67]. Genipin, isolated from Gardenia jasminoides fruits, was used successfully by Dare et al [68] as a less toxic alternative to glutaraldehyde for crosslinking of fibrin matrices and induce chondrogenic differentiation of human primary articular chondrocytes.…”

Section: Use Of Bifunctional Reagentsmentioning

confidence: 98%

“…1). These include Fibrin Microbeads (FMB) [29], Fibrin Films (FF) [30], Fibrin Discs (FD) [31], Fibrin Threads (FT) [32], Fibrin Microparticles (FMP) [33] Fibrin Nanoparticles (FNP) [34] and Fibrin Nano Constructs (FNC) [35]. More recently, whole blood plasma, induced to clot with help of endogenous thrombin and transglutaminase and formulated as plasma beads (PB) or plasma microparticles (PMPs), has also been studied for its therapeutic delivery potential [36,37].…”

Section: Components Of the Fibrin Matricesmentioning

confidence: 99%

See 1 more Smart Citation

Fibrin matrices: The versatile therapeutic delivery systems

Ahmad

Fatima

Hoque

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Section: Use Of Bifunctional Reagentsmentioning

confidence: 98%

Section: Components Of the Fibrin Matricesmentioning

confidence: 99%

Fibrin matrices: The versatile therapeutic delivery systems

Ahmad

Fatima

Hoque

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…The plasma beads are composed of recipient own blood plasma and are highly biocompatible and biodegradable material. 17 They can entrap both low and high molecular weight drugs and have an ability to stay longer in blood circulation. In fact, plasma beads could have an extra edge over other methods thus becoming a potential and safe drug-delivery vehicle.…”

Section: Introductionmentioning

confidence: 99%

Antifungal efficacy of amphotericin B encapsulated fibrin microsphere for treating Cryptococcus neoformans infection in Swiss albino mice

Khan

Jabeen

Alanazi

et al. 2016

The Brazilian Journal of Infectious Diseases

View full text Add to dashboard Cite

A natural and biocompatible fibrin microsphere is one of the most promising dual delivery vehicle as compared to other traditionally designed delivery modalities. It represents sustained delivery of encapsulated drug and is easily biodegradable in the blood circulation. In the present study, we evaluated the systemic augmentation of the antifungal activity of amphotericin B loaded in fibrin microsphere (AMB-fibrin microsphere) against cryptococcosis in Swiss albino mice. Mice infected with Cryptococcus neoformans were treated with 0.5mg/kg AMB-fibrin microsphere that was given alternately for 7 days. The antifungal potential of AMB-fibrin microsphere was assessed on the basis of reduction of cfu count in the systemic circulation and various vital organs of infected mice. The formulation was found to be highly effective in reducing intracellular pathogen from the experimental animals where fibrin microsphere significantly controlled the release of amphotericin B for longer time duration. The AMB-fibrin microsphere chemotherapy was significantly more effective than free amphotericin B in reducing the fungal burden and showed better survival efficacy (p<0.05). The current study demonstrating the use of novel amphotericin B loaded fibrin microsphere not only imparts protection to the encapsulated amphotericin B but also offers an effective strategy to decrease the drug associated toxicities.

show abstract

“…High-throughput screening has enabled the characterization of multivariate systems in a cost-and time-saving manner [27][28][29][30][31][32]. While several high-throughput screening platforms have been applied to assess the osteogenic potential of biomaterials [31,[33][34][35][36][37], those and other in vitro and in vivo high-throughput screening experiments have mostly been performed using platforms limited to static setups [38,39].…”

Section: Introductionmentioning

confidence: 99%

Screening of perfused combinatorial 3D microenvironments for cell culture

et al. 2019

View full text Add to dashboard Cite

Biomaterials combining biochemical and biophysical cues to establish close-to-extracellular matrix (ECM) models have been explored for cell expansion and differentiation purposes. Multivariate arrays are used as material-saving and rapid-to-analyze platforms, which enable selecting hit-spotted formulations targeting specific cellular responses. However, these systems often lack the ability to emulate dynamic mechanical aspects that occur in specific biological milieus and affect physiological phenomena including stem cells differentiation, tumor progression, or matrix modulation. We report a tailor-made strategy to address the combined effect of flow and biochemical composition of three-dimensional (3D) biomaterials on cellular response. We suggest a simple-to-implement device comprising (i) a perforated platform accommodating miniaturized 3D biomaterials and (ii) a bioreactor that enables the incorporation of the biomaterial-containing array into a disposable perfusion chamber. The system was upscaled to parallelizable setups, increasing the number of analyzed platforms per independent experiment. As a proof-of-concept, porous chitosan scaffolds with 1 mm diameter were functionalized with combinations of 5 ECM and cell-cell contact-mediating proteins, relevant for bone and dental regeneration, corresponding to 32 protein combinatorial formulations. Mesenchymal stem cells adhesion and production of an early osteogenic marker were assessed on-chip on static and under-flow dynamic perfusion conditions. Different hit-spotted biomaterial formulations were detected for the different flow regimes using direct image analysis. Cell-binding proteins still poorly explored as biomaterials componentsamelogenin and E-cadherin-were here shown as relevant cell response modulators. Their combination with ECM cell-binding proteins-fibronectin, vitronectin, and type 1 collagen-rendered specific biomaterial combinations with high cell adhesion and ALP production under flow. The developed versatile system may be targeted at widespread tissue regeneration applications, and as a disease model/drug screening platform. Statement of Significance A perfusion system that enables cell culture in arrays of three-dimensional biomaterials under dynamic flow is reported. The effect of 31 cell-binding protein combinations in the adhesion and alkaline phosphatase (ALP) production of mesenchymal stem cells was assessed using a single bioreactor chamber. Flow perfusion was not only assessed as a classical enhancer/accelerator of cell growth and early osteogenic differentiation. We hypothesized that flow may affect cell-protein interactions, and that key components driving cell response may differ under static or dynamic regimes. Indeed, hit-spotted formulations that elicited highest cell attachment and ALP production on static cell culture differed from the ones detected for dynamic flow assays. The impacting role of poorly studied proteins as E-cadherin and amelogenin as biomaterial components was highlighted.

show abstract

Fibrin-Based Drug Delivery Systems. II. The Preparation and Characterization of Microbeads

Cited by 8 publications

References 4 publications

Fibrin matrices: The versatile therapeutic delivery systems

Fibrin matrices: The versatile therapeutic delivery systems

Antifungal efficacy of amphotericin B encapsulated fibrin microsphere for treating Cryptococcus neoformans infection in Swiss albino mice

Screening of perfused combinatorial 3D microenvironments for cell culture

Contact Info

Product

Resources

About