Fiber-packed SPE tips based on electrospun fibers

Zhang, Yiyun; Kang, Xuejun; Chen, Liqin; Pan, Chao; Yao, Yingfang; Gu, Zhongze

doi:10.1007/s00216-008-2145-2

Cited by 55 publications

(39 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, an array pretreatment device based on PFSPE was designed for highthroughput sample extraction. PFSPE has been used for the extraction of target compounds (trazodone, nitrobenzene, 2-naphthol, benzene, n-butyl p-hydroxybenzoate, naphthalene, p-dichlorobenzene, and hair cortisol) from some aqueous medium at moderate pH in our previous articles [22][23][24]. In this article, PFSPE was successfully used to concentrate target compounds from strongly acid medium.…”

Section: Introductionmentioning

confidence: 91%

Application of nanofiber‐packed SPE for determination of salivary‐free cortisol using fluorescence precolumn derivatization and HPLC detection

Chen

Kang

Sun

et al. 2010

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Salivary cortisol has emerged as an easy-to-collect biologic marker of stress in many researches. In this study, we present a method for the determination of salivary-free cortisol using HPLC method with fluorescence precolumn derivatization, which is based on a novel extraction from the strongly acidic medium (fluorescent derivatives of cortisol in sulfuric acid medium) by electrospun polystyrene nanofibers packed SPE. For high-throughput sample extraction, an array pretreatment device based on nanofibers packed SPE micro-column was designed. The LOD of cortisol was 0.01 microg/L (S/N=3). The RSDs (n=6) for all analytes were below 8.0%, and the recoveries were 110, 102.4, and 99.4% (n=3) for saliva spiked with 0.1, 10, and 20 microg/L of cortisol, respectively. The proposed method was then successfully applied in the determination of free cortisol in human saliva. The salivary cortisol concentrations in the real samples ranged from 0.22 to 7.45 microg/L. The nanofiber-packed SPE overcame the low extraction recovery and bad clean-up effect of the conventional methods, and increased the sensitivity and selectivity of the method.

show abstract

Section: Introductionmentioning

confidence: 91%

Application of nanofiber‐packed SPE for determination of salivary‐free cortisol using fluorescence precolumn derivatization and HPLC detection

Chen

Kang

Sun

et al. 2010

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Their high specific surface areas (Liu et al 2010;Aguado et al 2009;Zhang et al 2008) and ease of functionalization with various groups for specific purposes (Kang et al 2007;Kang et al 2008;Yoshitake et al 2003) has increased their acceptance in the scientific community. The simplest and most flexible means of fabricating nanofibers is via electrospinning (Chigome et al 2011;Uyar and Besenbacher 2008;Greiner and Wendorff 2007;Ramakrishna et al 2006).…”

Section: -Hydroxypyrenementioning

confidence: 99%

Electrospun nanofiber sorbents for the pre-concentration of urinary 1-hydroxypyrene

et al. 2015

View full text Add to dashboard Cite

show abstract

“…16 Recently, a novel solid-phase extraction method based on electrospun polymer nanofibers as adsorbents to enrich target compounds has been developed. 17 Owing to their large surface area to volume ratio, electrospun nanofibers facilitate the miniaturization of SPE when they are packed into a pipette tip as the sorbent beds. With this packed-fiber solid-phase extraction (PFSPE) platform, the extraction capability could be efficiently improved and the volume of desorption solvent could be reduced to microliter levels.…”

Section: Introductionmentioning

confidence: 99%

Determination of Estrogens in Milk Using Polypyrrole Fiber-Mediated Solid-Phase Extraction Followed by High Performance Liquid Chromatography

Wei

Yan

Deng³

et al. 2018

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

A solid-phase extraction (SPE) method based on conductive polypyrrole (PPy) nanofibers which were fabricated by electrospinning and in situ polymerization was developed. PPy nanofibersmediated SPE followed by high performance liquid chromatography (HPLC) was used for the determination of three synthetic estrogens, namely diethylstilbestrol (DES), dienestrol (DIS), or hexestrol (HS) in milk sample. Extraction conditions including extraction nanofibers, donor solution pH and salt concentration were optimized. The target compounds were extracted from a 0.5 mL aqueous sample at pH 5.0 through PPy fibers, and then were eluted with 0.1 mL methanol. After extraction, the eluant was directly injected into an HPLC system for detection. Under the optimized extraction conditions, a large enrichment factor was achieved for three estrogens. The limit of detection (LOD) at a signal to noise ratio (S/N) of 3 ranged from 0.02 to 0.05 μg mL -1 for the estrogens in milk sample.Keywords: liquid dairy products, packed-fibers solid-phase extraction, high performance liquid chromatography, estrogens IntroductionEstrogens have been considered as one of the groups of analytes concerned in certain food matrices, especially in milk and other dairy products.1 Synthetic estrogens such as diethylstilbestrol (DES), dienestrol (DIS) and hexestrol (HS) are potent regulatory factors in physiological response medicine, and have been used widely as growth promoters in livestock and as a treatment for estrogen-deficiency disorders in veterinary medicine.A variety of techniques have been applied to determine estrogens. The abuse of estrogens is known to be strongly harmful to humans owing to their potential carcinogenic properties. The use of estrogens in food-producing animals and other products has been prohibited in many countries. Synchronously, more attention is paid to residue analysis of these estrogens that helps us control their illegal use.2-4 Generally, the determination of DES in wastewater has been carried out using gas chromatographymass spectrometry, 5,6 liquid chromatography-mass spectrometry 7,8 or high performance liquid chromatography equipped with diode array detection. [9][10][11] Since the concentration of DES in complex matrices is usually extremely low, a preconcentration step, such as solidphase extraction (SPE) and liquid-phase microextraction (LPME), is required. However, the lack of selectivity and low recovery are the main problems in the determination of estrogens by using common samples preconcentration. [12][13][14][15] Solid-phase extraction, because of its advantages such as less organic solvent consumption, simplicity, high recovery rate, easy operation and automation, has been Determination of Estrogens in Milk Using Polypyrrole Fiber-Mediated Solid-Phase Extraction J. Braz. Chem. Soc. 2138 widely applied to the extraction of different compounds. 16 Recently, a novel solid-phase extraction method based on electrospun polymer nanofibers as adsorbents to enrich target compounds has been developed. 17 Owing to t...

show abstract

Fiber-packed SPE tips based on electrospun fibers

Cited by 55 publications

References 17 publications

Application of nanofiber‐packed SPE for determination of salivary‐free cortisol using fluorescence precolumn derivatization and HPLC detection

Application of nanofiber‐packed SPE for determination of salivary‐free cortisol using fluorescence precolumn derivatization and HPLC detection

Electrospun nanofiber sorbents for the pre-concentration of urinary 1-hydroxypyrene

Determination of Estrogens in Milk Using Polypyrrole Fiber-Mediated Solid-Phase Extraction Followed by High Performance Liquid Chromatography

Contact Info

Product

Resources

About