Fiber-Optic Biosensor Employing Alexa-Fluor Conjugated Antibody for Detection of Escherichia coli O157:H7 from Ground Beef in Four Hours

Geng, Tao; Uknalis, Joseph; Tu, Su-I; Bhunia, Arun K.

doi:10.3390/s6080796

Cited by 54 publications

(45 citation statements)

References 27 publications

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“…This generates an optical signal, the strength of which is proportional to specific analyte binding. To convert these assays from a laboratory-based 96-well plate format to a smaller, more portable biosensor system, optical fibers have been employed for the detection of whole bacterial cells (35,36). Fiber optic biosensors (FOB) typically comprise a source of light which passes through optical fibers containing immobilized bioreceptors to a photon detector.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…The system holds potential to meet the need for simple, rapid, sensitive, and antigenspecific real-time detection for its structural design and assay principle. Several investigations have already been demonstrated using the same principle of the fluorescent immunoassay system, such as Escherichia coli O157:H7 (Geng et al 2006;DeMarco and Lim 2001), Salmonella (Valadez et al 2009), and Listeria monocytogenes (Geng et al 2004). However, to our knowledge, there has been no report about detection of environmental allergens using the fluorescence immunoassay system.…”

Section: Introductionmentioning

confidence: 92%

Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1)

Miyajima

Itabashi

Koshida

et al. 2011

Environ Monit Assess

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Immunoassay methods are generally used for measuring of allergenic substances. However, they need special facilities, skilled handling, and time-consuming procedure. In this work, a fiber-optic immunoassay system which could measure allergen by fluorescent intensities of immune complexes formed by allergens and fluorescently labeled antibodies was established. Immune complexes absorbed on the optical fiber probe surface, and excitation light was injected into the probe, then evanescent field is created in the proximity of the probe. The fluorophores were excited by the evanescent light, and fluorescence was detected by a photo diode. The target allergen detected by our system was Der f1 derived from Dermatophagoides farinae that is one of the house dust mite and major source of inhaled allergens. The fluorophore used labeling on detecting antibody was cyanine 5. The system enabled to detect and quantitatively determine of Der f1. The measurement range was from 0.24 to 250 ng/ml, and the result competes with ELISA. The measurement time was 16 min/sample. The immunoassay system was applied to measurement of Der f1 from actual dust samples. Calculated values of Der f1 showed good correlations between the fiber-optic fluoroimmunoassay and ELISA.

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“…A fluorescence-based fiber-optic biosensor for detecting E. coli O157:H7 in ground beef samples was developed by Geng et al [78]. The authors reported sensitivity of 10 3 CFU/ml in pure cultures and of 1 CFU/ml in artificially contaminated ground beef samples after 4 h enrichment using a sandwich immunoassay.…”

Section: Fluorescence-based Biosensorsmentioning

confidence: 99%

Microbiological Detectors for Food Safety Applications

Alocilja

Pal

2008

Wiley Handbook of Science and Technology for Homeland Security

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The use of microorganisms as biological weapons has long been reported in history. The most recent was the deliberate release of B. anthracis spores through the postal system in the United States in October 2001, resulting in 22 cases of anthrax and five deaths. Furthermore, there are up to 33 million cases of human illness each year from microbial pathogens in the food supply in the United States alone, with an associated cost of $2‐4 billion in 2006. The complexity of the US food supply chain from cradle to grave provides numerous entry points and routes in which (inadvertent and intentional) contaminants and pathogens can be introduced into the nation's food system. Many of these pathogenic microorganisms have a very low infectious dose to cause fatality. The most vulnerable populations are the very young, the elderly, and immunocompromised individuals. Although highly sensitive, current methods of detection are laborious, require highly skilled personnel, and typically require 2–7 days to obtain conclusive results. Their results are not available in the time‐scale desired in food defense, food quality assurance, or clinical laboratory procedures, which have safety, cost, and quality implications. Rapid on‐site point‐of‐care detection methods for pathogens have hence become a necessity. Biosensors can play a major role in addressing the combined needs for near real‐time diagnosis, specificity, sensitivity, portability, electronic data transmission, field‐based, ease of use, and low cost requirements. This chapter presents biosensors for rapid microbial detection in complex food matrices of concern to homeland security and food safety.

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Fiber-Optic Biosensor Employing Alexa-Fluor Conjugated Antibody for Detection of Escherichia coli O157:H7 from Ground Beef in Four Hours

Cited by 54 publications

References 27 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1)

Microbiological Detectors for Food Safety Applications

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