Fiber interferometer for hybrid optical and optoacoustic intravital microscopy

Shnaiderman, Rami; Wissmeyer, Georg; Seeger, Markus; Soliman, Dominik; Estrada, Héctor; Razansky, Daniel; Rosenthal, Amir; Ntziachristos, Vasilis

doi:10.1364/optica.4.001180

Cited by 46 publications

(32 citation statements)

References 40 publications

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“…It sensed a broad variety of biologically relevant compounds, e.g. vasculature, collagen, elastin, keratinocytes, hair follicle, sebaceous glands, and lipid embeddings [2,7,[10][11][12]. This allowed us to study small model organisms such as fish larvae and mouse ears in vivo (see Fig.…”

Section: Resultsmentioning

confidence: 99%

In-vivo hybrid microscopy of small model organisms

Seeger¹,

Westmeyer²,

Ntziachristos³

2019

Opto-Acoustic Methods and Applications in Biophotonics IV

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We present the investigation of in vivo small model organisms, which are well established in biological and biomedical research, using a hybrid multiphoton and optoacoustic microscope (HyMPOM). The unique capabilities of HyMPOM for multimodal and potentially label-free signal acquisition, high resolution, as well as deep and fast imaging allow extraction of detailed information across large areas of living tissue on the microscale. Applying HyMPOM to living zebrafish-like fish larvae allowed exploration of the structural composition of the entire brain, including the brain vasculature and the neuronal network. Applying HyMPOM to the ears of living mice enabled accurate imaging of vasculature, connective tissue, keratinocytes, and sebaceous glands. The hybrid microscope proposed here constitutes a novel approach to explore small model organisms in vivo in great detail by revealing the spatial distribution and interplay of various tissue compartments on the microscale.

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Section: Resultsmentioning

confidence: 99%

In-vivo hybrid microscopy of small model organisms

Seeger¹,

Westmeyer²,

Ntziachristos³

2019

Opto-Acoustic Methods and Applications in Biophotonics IV

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“…In resonator-based technique, a micron-scale optical resonator detects ultrasound waves (see Figure 4 a(iv)); using this technique, miniaturization of the ultrasound detection unit is feasible. The optical resonator geometries that are most frequently used in photoacoustic imaging are Fabry–Pérot interferometers (FP) [ 161 , 162 , 163 ], micro-ring resonators (MRRs) [ 164 , 165 , 166 ], and π-phase-shifted fiber Bragg gratings (π-FBGs) [ 167 , 168 , 169 ]. Refractometric methods can be classified as intensity sensitive, beam deflectometry, and phase sensitive [ 41 , 42 ].…”

Section: Optical Ultrasound Detection Technologiesmentioning

confidence: 99%

Overview of Ultrasound Detection Technologies for Photoacoustic Imaging

2020

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Ultrasound detection is one of the major components of photoacoustic imaging systems. Advancement in ultrasound transducer technology has a significant impact on the translation of photoacoustic imaging to the clinic. Here, we present an overview on various ultrasound transducer technologies including conventional piezoelectric and micromachined transducers, as well as optical ultrasound detection technology. We explain the core components of each technology, their working principle, and describe their manufacturing process. We then quantitatively compare their performance when they are used in the receive mode of a photoacoustic imaging system.

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“…The lowest detectable concentration is 2 μg/L, whereas the cut-off level is 15 μg/L serum [28]. An interesting approach is the combination of optical and opto-acoustic microscopy to image thin samples to make it more accessible to the biomedical community [202]. In this opto-acousting microscopy, a protein transmission mode phase-shifted fiber Bragg grating interferometry was used.…”

Section: Structured Fibers and Waveguidesmentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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Fiber interferometer for hybrid optical and optoacoustic intravital microscopy

Cited by 46 publications

References 40 publications

In-vivo hybrid microscopy of small model organisms

In-vivo hybrid microscopy of small model organisms

Overview of Ultrasound Detection Technologies for Photoacoustic Imaging

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

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