Fiber-in-tube solid-phase extraction using a polyetheretherketone capillary, and effective on-line coupling with microcolumn liquid chromatography

Abstract

On-line coupling of fiber-in-tube solid-phase extraction (SPE), using poJyetheretherketone (PEEK) tubing, with microscale liquid chromatography (LC) has been investigated. PEEK tubing and polymer filaments are introduced as new medium for extraction of phthalates -diethyl phthalate (DEP), dipropyl phthalate (DPP), and dibutyl phthalate (DBP). Approximately 300 pieces of polymer filamentwere packed into a PEEK capillary 100 mm long (0.25 mm i.d.) and the capillarywas installed in the injection valve as the sample loop. Desorption and injection of the analytes were performed simultaneouslywithout use of the desorption solvent required by conventional SPE methods.The results obtained showed that effective on-line coupling of this sample-preparation method with liquid chromatography and other liquid-phase separation techniques can be accomplished for the high-throughput analysis of complex mixtures, without large consumption of organic solvents. The limits of detection for these phthalates, approximately 1.0 ng mL 1, and the extraction time, 10 min, indicate the future possibilities of this technique.

Polymer-Coated Fibrous Stationary Phases in Packed-Capillary Gas Chromatography

“…The study for more extensive applications of various polymercoated fiber-packed capillaries, including the use as an extraction medium for trace amount of organics in complex sample mixture, [20][21][22][23][24][25][26][27][28] is currently underway in our laboratory along with the extensive comparisons of other heat-and …”

“…Saito et al have also reported the successful applications of fibrous polymeric material as an extraction medium for novel miniaturized sample preparations specially designed for the on-line coupling to microcolumn liquid phase separation methods. [14][15][16][17][18][19][20][21][22] On the basis of the above successful applications of fibrous materials as the stationary phase and the extraction medium, fiber-packed fused-silica capillaries have been introduced in our previous study for the separation of volatile compounds in GC. 23 In this work, polymer coating onto the packed-filaments in a capillary was investigated using several conventional polymeric materials as the liquid-phase for capillary GC columns.…”

Organic monolith frits encased in polyether ether ketone tubing with improved durability for liquid chromatography

“…Such study is planned in the future. Interestingly, long bare PEEK tubing and fiber‐in PEEK tubing were employed as SPE media.…”

Solid‐phase microextraction: a powerful sample preparation tool prior to mass spectrometric analysis

“…Note in particular new coatings such as ADS, MIP and PPY, discussed in detail recently 26. To obtain higher extraction efficiency and extend the method to microscale applications, different techniques such as wire‐in‐tube28, 29 or fibre‐in‐tube28, 30–33 have also been developed. By insertion of a stainless‐steel wire into the extraction capillary of in‐tube SPME, the internal volume of the capillary can be significally reduced while the surface area of the polymeric coating material remains the same.…”

“…Miniaturized sample preparation methods have been regarded as the most attractive techniques for the pretreatment of complex sample mixtures prior to the chromatographic process. The main advantages of the miniaturized systems are high‐speed analysis with high efficiency, low cost and environmentally friendly operation due to the minimal or no solvent consumption, and highly selective analysis by developing tailored systems designed for particular applications 28–33. Extracting very small amount of samples (10–35 µl) is difficult but in a biological sample treatment sometimes the sample amount is very limited (saliva, mouse urine, etc.)…”