2016
DOI: 10.1016/j.msec.2016.02.040
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Fiber from ramie plant (Boehmeria nivea): A novel suture biomaterial

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Cited by 78 publications
(55 citation statements)
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“…Tetrazolium salt assay (MTT) was used to determine the cytotoxic concentration of ZJME and ZJWF (Kalita S. et al, 2015, 2016; Kandimalla et al, 2016b; Konwar et al, 2016). Briefly, the cells from the culture flask were trypsinized and seeded in 96-well plate at 5 × 10 3 cells/well in a culture medium and incubated in the CO 2 incubator at 37°C for 24 h. Then the culture medium from the wells was replaced with new media and ZJME and ZJWF at different concentrations (5, 25, 50,125, 250, and 500 μg/mL).…”
Section: Methodsmentioning
confidence: 99%
“…Tetrazolium salt assay (MTT) was used to determine the cytotoxic concentration of ZJME and ZJWF (Kalita S. et al, 2015, 2016; Kandimalla et al, 2016b; Konwar et al, 2016). Briefly, the cells from the culture flask were trypsinized and seeded in 96-well plate at 5 × 10 3 cells/well in a culture medium and incubated in the CO 2 incubator at 37°C for 24 h. Then the culture medium from the wells was replaced with new media and ZJME and ZJWF at different concentrations (5, 25, 50,125, 250, and 500 μg/mL).…”
Section: Methodsmentioning
confidence: 99%
“…The next day morning culture media from the plate was replaced with fresh media. Cells were pretreated with plant fractions at different concentrations (0, 0.5, 5, and 50 μg/ml) and incubated for 12 h before the cells were exposed to 100 μM H 2 O 2 for next 12 h. After 24 h of the total incubation period, cell viability was evaluated by MTT assay (Kalita et al, 2015; Kalita S. et al, 2016; Kandimalla et al, 2016b). To each well 10 μl of 4 mg/ml MTT solution was added and incubated at 37°C for 2 h. After the incubation period, the MTT solution was removed from the wells and 100 μl of DMSO solution was added to dissolve the formazan crystals.…”
Section: Methodsmentioning
confidence: 99%
“…[20][21][22] The protocol for experiments on human blood samples was approved by the Institutional Human Ethics Committee (IHEC), IASST, Guwahati, Assam, India. Informed consent was obtained from the human volunteer to conduct the experiments.…”
Section: Biocompatibility Evaluation Of Nanohybridsmentioning
confidence: 99%