Fiber evanescent wave spectroscopy using the mid-infrared provides useful fingerprints for metabolic profiling in humans

Abstract

Fiber evanescent wave spectroscopy (FEWS) explores the mid-infrared domain, providing information on functional chemical groups represented in the sample. Our goal is to evaluate whether spectral fingerprints obtained by FEWS might orientate clinical diagnosis. Serum samples from normal volunteers and from four groups of patients with metabolic abnormalities are analyzed by FEWS. These groups consist of iron overloaded genetic hemochromatosis (GH), iron depleted GH, cirrhosis, and dysmetabolic hepatosiderosis (DYSH). A partial least squares (PLS) logistic method is used in a training group to create a classification algorithm, thereafter applied to a test group. Patients with cirrhosis or DYSH, two groups exhibiting important metabolic disturbances, are clearly discriminated from control groups with AUROC values of 0.94+/-0.05 and 0.90+/-0.06, and sensibility/specificity of 8684% and 8787%, respectively. When pooling all groups, the PLS method contributes to discriminate controls, cirrhotic, and dysmetabolic patients. Our data demonstrate that metabolic profiling using infrared FEWS is a possible way to investigate metabolic alterations in patients.

Une nouvelle méthode pour le diagnostic rapide d’arthrite septique utilisant la spectroscopie infrarouge

“…Cette spectroscopie dans le moyen infrarouge, par onde évanescente, a montré son intérêt pour discriminer des états biologiques expérimentaux ou des maladies humaines [18][19][20][21][22][23].…”

“…La méthode permet ainsi de recueillir une empreinte métabolique des échantillons. Il est ensuite possible d'utiliser des modèles statistiques pour détecter des variations de cette empreinte, et de mettre à profit ces variations comme marqueur d'un état pathologique ou sain [18][19][20][21][22][23].…”

Chalcogenide glass fibers: Optical window tailoring and suitability for bio-chemical sensing

“…Strong signals can therefore be collected which enable advanced spectral analysis for selective detection [38,43]. These fibers are now reaching the stage of commercial development for the design of bio-medical sensors but much research is still ongoing to optimize their attenuation and optical window.…”

“…The complex vibrational signature of bio-molecules in the 6-12 lm region offers a very effective mean of identifying, monitoring or diagnosing bio-molecules and tissues [10,11,[39][40][41]. In that respect chalcogenide glass bio-sensors have been successfully used to diagnose cancerous tissues [42] or human serum [43], as well as to monitor the swarming of bacterial film [44] or the metabolism of live lung cell cultures [13] and to selectively identify different types of bacteria [15] or proteins [33].…”

The Development of Advanced Optical Fibers for Long-Wave Infrared Transmission

“…In particular, infrared fibers with transmission down to the long-wave infrared up to 16 microns are of special interest for applications such as micro-organism detection [1][2][3], environmental monitoring [4][5][6], medical diagnostic [7,8], thermal imaging through fiber bundles [9], laser surgery delivery at 10.6 microns [10], CO 2 gas monitoring for space exploration [11][12][13] as well as optical communication in the second atmospheric window at 8-12 microns.…”