Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant

Abstract

2014. Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant. Can. J. Anim. Sci. 94: 697Á704. This study investigated the effects of two fibrolytic enzyme products, applied at baling alone or in combination with a ferulic acid esterase-producing bacterial additive, on the ensilage dynamics, chemical composition and digestibility of alfalfa haylage. Five replicate wrapped bales were produced with one of five treatments, including an untreated control, and one of two fibrolytic enzyme products (EN1 and EN2) applied either alone or in combination with a ferulic-acid producing bacterial additive (FAEI). No effect of treatment was observed on the neutral detergent fiber (NDF) (P00.889) or acid detergent fiber (ADF) (P00.065) concentrations of haylage after ensilage, but haylage produced using fibrolytic enzyme products underwent greater (PB0.018) increases in temperature following exposure to aerobic conditions. Haylages produced with fibrolytic enzyme products had a greater (PB0.001) in vitro NDF degradability (NDFD) than untreated haylage. The use of fibrolytic enzymes applied to alfalfa haylage at ensiling increased the NDFD, despite minimal effects on the chemical composition of the herbage. However, the greater aerobic deterioration of fibrolytic enzyme-treated bales indicates higher dry matter losses during aerobic exposure. The use of FAEI with fibrolytic enzymes did not further enhance the effects of fibrolytic-enzyme treatments.

Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices

“…This might support that inoculation of FD and FDest decreased NDF concentration of rye silage in the present study. In agreement, Lynch et al [6] also reported that NDF concentration of alfalfa silage was lower in mixture of cellulase and xylanase treatment than that of control (32.1% vs 33.5%). Silage inoculated with FD increased both IVDMD and IVNDFD of rye silage in the present study.…”

“…Application of lactic acid bacteria (LAB) in silage has been widely used to improve the fermentation quality [5] and digestibility of silage [6,7]. Several strains of LAB have abilities of producing antifungal [8,9] and fibrinolytic enzymes [9,10], which could increase the quality of rye silage, especially harvested at the dough stage.…”

Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications

“…Ferulic and hydroxycinnamic acids themselves can promote health in animals [148, 149]; however, feruloylation in plant cell walls particularly in a high forage diet is among the major inhibitory factors for the ruminant digestive system. Addition of FAEs or enzyme cocktails containing FAEs can improve the access of main chain degrading enzymes resulting in improved fiber digestion and bioavailability of phytonutrients, accelerating animal growth (e.g., [150, 151]), as well as reducing immunological stress [152]. …”

Effects of Ferulic Acid Esterase-Producing Lactic Acid Bacteria and Storage Temperature on the Fermentation Quality, In Vitro Digestibility and Phenolic Acid Extraction Yields of Sorghum (Sorghum bicolor L.) Silage

“…FAE-producing LAB makes cross-linked polysaccharides more fragile and thus more easily degraded by rumen due to the synergy between esterases and main-chain degrading enzymes [ 10 ]. Several research studies have attempted to study the effects of FAE-producing LAB strains on ensiling and fiber degradability, and some of the findings have shown that LAB-treated silage exhibits higher neutral detergent fiber digestibility (NDFD) [ 11 , 12 , 13 ].…”