Fiber attenuated total reflection infrared spectroscopy of kidney tissue during live surgery

Abstract

More than 90% of solid kidney tumors are cancerous and have to be treated by surgical resection where surgical outcomes and patient prognosis are dependent on the tumor discrimination. The development of alternative approaches based on a new generation of fiber attenuated total reflection (ATR) probes could aid tumor identification even under intrasurgical conditions. Herein, fiber ATR IR spectroscopy is employed to distinguish normal and cancerous kidney tissues. Freshly resected tissue samples from 34 patients are investigated under nearly native conditions. Spectral marker bands that allow a reliable discrimination between tumor and normal tissue are identified by a supervised classification algorithm. The absorbance values of the bands at 1025, 1155 and 1240 cm −1 assigned to glycogen and fructose 1,6-bisphosphatase are used as the clearest markers for the tissue discrimination. Absorbance threshold values for tumor and normal tissue are determined by discriminant analysis. This new approach allows the surgeon to make a clinical diagnosis.

Fast and label-free intraoperative discrimination of malignant pancreatic tissue by attenuated total reflection infrared spectroscopy

“…As refractive index for the Ge crystal n=4 was set. Details of the system are described elsewhere 17 , 18 …”

Fiber-based ATR-IR spectroscopy for an intraoperative diagnosis of normal pancreatic tissue, pancreatitis, and malignant pancreatic lesions

“…Measurements of freshly resected human pancreatic tissue spectra were performed. Details of the ATR IR fiber spectroscopy system are described elsewhere 6,7 . For each sample, several tissue slices were cut, and spectra were measured from these freshly cut surfaces.…”

Clinical applications of infrared and Raman spectroscopy in the fields of cancer and infectious diseases

“…Sablinskas et al [96] employed the less explored technique of Fiber ATR-IR to examine fresh kidney tissue, resected from patients undergoing surgery. Spectra of tissue were measured inside the operation theater, immediately after resection, using an ATR silver halide fiber probe.…”