FGFR3 is expressed by human primordial germ cells and is repressed after meiotic initiation to form primordial oocytes

Chitiashvili, Tsotne; Hsu, Fei‐Man; Dror, Iris; Plath, Kathrin; Clark, Amander T.

doi:10.1016/j.stemcr.2022.04.015

Cited by 8 publications

(2 citation statements)

References 36 publications

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“…Nevertheless, it is perhaps different from the origin of hPGCs, that PGCs in cynomolgus monkey might emerge in amnion. 87,95 In general, hPGCs/hPGCLCs express a range of types of key genes, including (1) pluripotency markers: NANOG, POU5F1, ALPL, KLF4, LIN28, KIT, NANOS3, SSEA-1, SSEA-4, DPPA3 (also called as STELLA), and ZFP42 (also known as REX1), 17,20,21,[161][162][163][164][165][166][167] (2) cell-surface makers: CD38, EPCAM, ITGA6 (INTEGRIN alpha 6), ITGB3, FGFR3, KIT, and ALPL, 20,21,167,168 (3) germline markers: SOX17, BLIMP1 (also known as PRDM1), TFAP2C, PRDM14, DDX4 (also known as VASA), DAZL, and TCL1A, 20,21,167,[169][170][171][172] (4) amnion-related genes: CDX2 and GATA3, 24 (5) mesoderm markers: EOMES, NODAL, SP5, and T, 20,21 (6) transcription factors: SOX17, BLIMP1, SOX15, GATA4, PRDM14, SALL4, and UTF1, 20,21,[172][173][174][175] and ( 7) epigenetic regulation factors: DNA demethylation dioxygenases (TET1, TET2, and TET3), protein arginine methyltransferase 5(PRMT5), and DND microRNAmediated repression inhibitor 1(DND1). 20,167,176,177 The types and expression patterns of these marker genes reflect corresponding states of hPGC development and cell identity.…”

Section: Regulation Of Hpgc Specificationmentioning

confidence: 99%

Germline stem cells in human

Cheng

Shang

Zhou

2022

Sig Transduct Target Ther

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The germline cells are essential for the propagation of human beings, thus essential for the survival of mankind. The germline stem cells, as a unique cell type, generate various states of germ stem cells and then differentiate into specialized cells, spermatozoa and ova, for producing offspring, while self-renew to generate more stem cells. Abnormal development of germline stem cells often causes severe diseases in humans, including infertility and cancer. Primordial germ cells (PGCs) first emerge during early embryonic development, migrate into the gentile ridge, and then join in the formation of gonads. In males, they differentiate into spermatogonial stem cells, which give rise to spermatozoa via meiosis from the onset of puberty, while in females, the female germline stem cells (FGSCs) retain stemness in the ovary and initiate meiosis to generate oocytes. Primordial germ cell-like cells (PGCLCs) can be induced in vitro from embryonic stem cells or induced pluripotent stem cells. In this review, we focus on current advances in these embryonic and adult germline stem cells, and the induced PGCLCs in humans, provide an overview of molecular mechanisms underlying the development and differentiation of the germline stem cells and outline their physiological functions, pathological implications, and clinical applications.

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Section: Regulation Of Hpgc Specificationmentioning

confidence: 99%

Germline stem cells in human

Cheng

Shang

Zhou

2022

Sig Transduct Target Ther

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“…Platelet derived growth factor which stimulates the germinal cell and regulates paracrine and autocrine functions [10]. These "growth factors" help to decrease testicular tissue ischemia, increase the width of seminal producing tubules which keep the epithelium of germinal healthy [11]. This study used rats as model to identify if a count and abnormalities of spermatozoa in addition to testicular tissue changed after CP treatment, and evaluate the ability of PRP to regenerate damaged structure of testicular tissue.…”

Section: Introductionmentioning

confidence: 99%

Evaluation Intra-testicular Injection of Platelet-Rich Plasma in Restoration Male Rat Fertility Affected By Cisplatin

2022

pnr

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Cisplatin is considered the first-line chemotherapy, reducing its cytotoxicity is still unmet need. Platelet-rich plasma (PRP) has a potential effect on tissue repair through regeneration and differentiation of tissue progenitor cells. Here, we used PRP as a biomaterial to protect and regenerate testicular tissue against cisplatin toxicity. PRP was applied locally using intra-testicular injection as a single dose per week for three following the cisplatin treatment (intra-peritoneum injection). Cisplatin treatment alone and PRP intra-testicular injection alone were used as control groups. The results show that PRP treatment was able to restore sperm counts and sperm morphology following cisplatin exposure. PRP treatment significantly reintited reproductive hormonal balance in affected rats with cisplatin. The results also showed that PRP treatment minimized histological damaged in testicular tissue resulting by the cisplatin, however, also increasing the number of spermatocytes in all stages of spermatogenesis. As well as elevation in the number of leydig cells and thickness of interstitial tissue was restored. This indicates the potential role of PRP in tissue regeneration in addition to tissue repair which may provide a promising use of PRP in different aspects related to chemotherapy.

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