FGF9/FGFR1 promotes cell proliferation, epithelial-mesenchymal transition, M2 macrophage infiltration and liver metastasis of lung cancer

Chang, Ming‐Chih; Wu, Suzhen; Yang, Su–Hua; Wu, Chin-Shan; Wang, Chia‐Yih; Huang, Bu‐Miin

doi:10.1016/j.tranon.2021.101208

Cited by 28 publications

(21 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis results of immune-related genes in HCC showed that NR0B1 and FGF9 were risk factors for HCC, and experiments confirmed that NR0B1 and FGF9 could enhance the migration ability of macrophages. The study by Chang et al confirmed that FGF9 can enhance the immune infiltration ability of M2 macrophages [ 31 ]. KEGG enrichment analysis demonstrated that IRGs were mainly enriched in immune-related pathways such as chemokine signaling pathway, which can confirm that the prognostic model constructed according to immune-related genes of HCC in this study has high reliability.…”

Section: Discussionmentioning

confidence: 99%

Prediction of Prognosis in Patients with Hepatocellular Carcinoma Based on Molecular Subtypes of Immune Genes

Shen

et al. 2022

Gastroenterology Research and Practice

View full text Add to dashboard Cite

For those patients with hepatocellular carcinoma (HCC), it is really a heavy burden. Herein, the immune genes of HCC were analyzed in groups to determine prognostic biomarkers related to immune genes in HCC. The mRNA data, clinical data in TCGA-LIHC dataset, and immune gene in the ImmPort database were collected for the combining usage with K -means concordance clustering to cluster HCC patients according to the immune gene matrix. Based on ssGSEA analysis result, HCC patients were sorted into high- and low-immune subtypes, and survival curve presented that patients in high-immune subtypes had a better prognosis. Subsequently, differential expression analysis was performed to obtain immune-related differentially expressed genes (IRGs). Cox and lasso analyses were performed for obtaining five optimal immune genes related to prognosis, and a risk assessment model was then established. Patient samples in the training and validation sets were, respectively, divided into high- and low-risk groups. K - M survival curves presented a better prognosis of patients in the low-risk group than in the high-risk group. The ROC curve indicated that this model was finely used for the prediction of prognosis. In addition, immune infiltration assessment revealed that NR0B1 and FGF9 had potential to impact the tumor immune microenvironment. Finally, using qRT-PCR and transwell assays, it was demonstrated that the macrophage chemotaxis was enhanced when NR0B1 and FGF9 were highly expressed in HCC cells. In general, a 5-gene prognostic risk assessment model was constructed based on immune genes and bioinformatics analysis methods, which provides some reference for the prognosis of HCC as well as immunotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Prediction of Prognosis in Patients with Hepatocellular Carcinoma Based on Molecular Subtypes of Immune Genes

Shen

et al. 2022

Gastroenterology Research and Practice

View full text Add to dashboard Cite

show abstract

“…The supernatants were then harvested and stored at -80 ˚C until further use. The protein concentration of the cell lysates was determined using a Micro BCA assay (44,45). For western blot analysis, ~30 µg lysates per lane were resolved on a 12% SDS-PAGE gel with standard running buffer (24 mM Tris/HCl, 0.19 M glycine, 0.5% SDS, pH 8.3) at 25˚C, and were subsequently transferred to PVDF membranes at 4˚C.…”

Section: Methodsmentioning

confidence: 99%

Arsenic compounds induce apoptosis by activating the MAPK and caspase pathways in FaDu oral squamous carcinoma cells

Lan

Chu

et al. 2022

Int J Oncol

Self Cite

View full text Add to dashboard Cite

For a number of years, oral cancer has remained in the top ten most common types of cancer, with an incidence rate that is steadily increasing. In total, ~75% oral cancer cases are associated with lifestyle factors, including uncontrolled alcohol consumption, betel and tobacco chewing, and the excessive use of tobacco. Notably, betel chewing is highly associated with oral cancer in Southeast Asia. Arsenic is a key environmental toxicant; however, arsenic trioxide has been used as a medicine for the treatment of acute promyelocytic leukemia, highlighting its anticancer properties. The present study aimed to investigate the role of arsenic compounds in the treatment of cancer, using FaDu oral squamous carcinoma cells treated with sodium arsenite (NaAsO 2 ) and dimethyl arsenic acid (DMA). The results demonstrated that FaDu cells exhibited membrane blebbing phenomena and high levels of apoptosis following treatment with 10 µM NaAsO 2 and 1 mM DMA for 24 h. The results of cell viability assay demonstrated that the rate of FaDu cell survival was markedly reduced as the concentration of arsenic compounds increased from 10 to 100 µM NaAsO 2 , and 1 to 100 mM DMA. Moreover, flow cytometry was carried out to further examine the effects of arsenic compounds on FaDu cell cycle regulation; the results revealed that treatment with NaAsO 2 and DMA led to a significant increase in the percentage of FaDu cells in the sub-G1 and G2/M phases of the cell cycle. An Annexin V/PI double staining assay was subsequently performed to verify the levels of FaDu cell apoptosis following treatment with arsenic compounds. Furthermore, the results of the western blot analyses revealed that the expression levels of caspase-8, -9 and -3, and poly ADP-ribose polymerase, as well the levels of phosphorylated JNK and ERK1/2 were increased following treatment with NaAsO 2 and DMA in the FaDu cells. On the whole, the results of the present study revealed that treatment with NaAsO 2 and DMA promoted the apoptosis of FaDu oral cancer cells, by activating MAPK pathways, as well as the extrinsic and intrinsic apoptotic pathways.

show abstract

“…73 FGFRs are involved in various biological processes such as cell proliferation, migration and EndMT. 74,75 FGFR1, a key anti-EndMT molecule, is an endothelial receptor required for counteracting EndMT. 76 Chen et al 77 reported that endothelial FGFR1 deficiency was associated with increased TGFβ signalling and Smad2 activation, which exacerbates EndMT through enhanced TGFβ signalling.…”

Section: Fgfr1 Signalling Pathwaymentioning

confidence: 99%

Research progress of endothelial‐mesenchymal transition in diabetic kidney disease

Chen

Zou

Lü

et al. 2022

J Cellular Molecular Medi

View full text Add to dashboard Cite

Renal fibrosis is an important pathological feature of diabetic kidney disease (DKD), manifested as tubular interstitial fibrosis, tubular atrophy, glomerulosclerosis and damage to the normal structure of the kidney. Renal fibrosis can eventually develop into renal failure. A better understanding of renal fibrosis in DKD is needed due to clinical limitations of current anti‐fibrotic drugs in terms of effectiveness, cost‐effectiveness and side effects. Fibrosis is characterized by local excessive deposition of extracellular matrix, which is derived from activated myofibroblasts to increase its production or specific tissue inhibitors of metalloproteinases to reduce its degradation. In recent years, endothelial‐mesenchymal transition (EndMT) has gradually integrated into the pathogenesis of fibrosis. In animal models of diabetic kidney disease, it has been found that EndMT is involved in the formation of renal fibrosis and multiple signalling pathways such as TGF‐β signalling pathway, Wnt signalling pathway and non‐coding RNA network participate in the regulation of EndMT during fibrosis. Here, we mainly review EndMT regulation and targeted therapy of renal fibrosis in DKD.

show abstract

FGF9/FGFR1 promotes cell proliferation, epithelial-mesenchymal transition, M2 macrophage infiltration and liver metastasis of lung cancer

Cited by 28 publications

References 75 publications

Prediction of Prognosis in Patients with Hepatocellular Carcinoma Based on Molecular Subtypes of Immune Genes

Prediction of Prognosis in Patients with Hepatocellular Carcinoma Based on Molecular Subtypes of Immune Genes

Arsenic compounds induce apoptosis by activating the MAPK and caspase pathways in FaDu oral squamous carcinoma cells

Research progress of endothelial‐mesenchymal transition in diabetic kidney disease

Contact Info

Product

Resources

About