FGF21-protection against fructose-induced lipid accretion and oxidative stress is influenced by maternal nutrition in male progeny

Liquid carbohydrate intake modifies transsulfuration pathway both in pregnant rats and in their male descendants

“…In addition, reductions in H2S production have been related to insulin resistance in adipose tissue (10) and, interestingly, our animals which were fed liquid fructose showed such a decrease. We were not able to detect systemic insulin resistance in these male rats (18), but it could possibly exist locally, in adipose tissue.…”

“…Beneficial effects of H2S in cardiovascular events have been widely studied (37,38), so a reduction in the gasotransmitter levels could be one of the links between fructose consumption and symptoms of CVD and MetS. In fact, we have previously reported that fructose intake provokes characteristic features of MetS, such as oxidative stress and lipid abnormalities, in the adult offspring and that these imbalances are influenced by maternal intake of carbohydrates (18,19). However, H2S plasma levels were not reduced by fructose consumption independently of that had been taken by their mothers.…”

Maternal Fructose Intake Increases Liver H2S Synthesis but Exarcebates its Fructose‐Induced Decrease in Female Progeny

“…[5] The males of each litter were used for a separate experiment. [4,45] Determinations: Plasma aliquots were used to measure homocysteine and urea by enzymatic colorimetric tests using commercial kits (Spinreact, Girona, Spain). An enzymatic kit using chemiluminescent immunoanalysis on microparticles (Architect, Abbot, Ireland) was used for the quantitative determination of folic acid in plasma.…”

Liquid carbohydrate intake modifies transsulfuration pathway both in pregnant rats and in their male descendants

“…The other half of the male progeny was subjected to the next protocol: First, they were weighed and an aliquot of plasma was obtained from the tail in order to confirm that the values between the experimental groups both for body weight and for several analytes (glucose, triglycerides, non-esterified fatty acids, etc) were similar (18). Later, independently from the experimental group of mothers to which they had been born, they were maintained on solid pellets and supplied with drinking water containing 10%…”

“…Prior to sacrifice, food was removed at a.m. The period with fructose supplementation was selected to last days (from 240 to 261 days of age) as previously described (18). Liver and lumbar and epididymal adipose tissues were immediately removed, placed in liquid nitrogen and kept at -80 ºC until analysis.…”