FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells

Nakao, Kazuhisa; Itoh, Makoto; Tomita, Yusuke; Tomooka, Yasuhiro; Tsuji, Takashi

doi:10.1016/j.bbrc.2004.10.136

Cited by 56 publications

(59 citation statements)

References 35 publications

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“…The chemical treatment of dentin (e.g., by EDTA) may solubilize various noncollagenous dentin matrix components and growth factors, such as transforming growth factor-β1, which may have an inductive effect on the differentiation of odontoblast progenitor cells (Smith 2002). With regard to the promotion of pulp cell proliferation on dentin before their differentiation into odontoblasts, other avenues may be explored in the future (Nakao et al 2004;Nakashima et al 2004).…”

Section: Discussionmentioning

confidence: 98%

“…First we wished to determine the difference, if any, of pulp cell phenotypes isolated by two methods, viz., enzyme digestion and the outgrowth method. The latter has been widely used by many investigators to study various aspects of pulp cell physiology Hosoya et al 1996;Nakao et al 2004;Patel et al Fig. 9 Collagen gel contraction assays.…”

Section: Discussionmentioning

confidence: 99%

“…Pulp cells have been isolated either by the well-known outgrowth method, i.e., cells outgrow from pulp tissue explants (About et al 2000;Couble et al 2000;Nakao et al 2004;Saito et al 2004;Tsukamoto et al 1992) or by enzyme digestion (collagenase/dispase/trypsin; Gronthos et al 2000;Nakashima 1991;Onishi et al 1999). However, no report has compared the cells derived from the two isolation methods.…”

Section: Introductionmentioning

confidence: 99%

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In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

et al. 2006

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Our purpose was to characterize human dental pulp cells isolated by various methods and to examine the behavior of cells grown under various conditions for the purpose of pulp/dentin tissue engineering and regeneration. We compared the growth of human pulp cells isolated by either enzyme digestion or the outgrowth method. Expression of dentin sialophosphoprotein, Cbfa1, and two types of collagen (I and III) in these cells was examined by Western blot or reverse transcription/polymerase chain reaction. Growth of pulp cells on dentin and in collagen gel was also characterized. We found that different isolation methods give rise to different populations or lineages of pulp cells during in vitro passage based on their collagen gene expression patterns. Cells isolated by enzymedigestion had a higher proliferation rate than those isolated by outgrowth. Pulp cells did not proliferate or grew minimally on chemically and mechanically treated dentin surface and appeared to establish an odontoblast-like morphology with a cytoplasmic process extending into a dentinal tubule as revealed by scanning electron microscopy. The contraction of the collagen matrix caused by pulp cells was dramatic: down to 34% on day 14. Our data indicate that (1) the choice of the pulp cell isolation method may affect the distribution of the obtained cell populations, (2) a treated dentin surface might still promote odontoblast differentiation, and (3) a collagen matrix may not be a suitable scaffold for pulp tissue regeneration because of the marked contraction caused by pulp cells in the matrix. The present study thus provides important information and a basis for further investigations pre-requisite to establishing pulp tissue engineering/regeneration protocols.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

et al. 2006

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“…Additionally, we previously reported that these calcifications are directly induced by the pulp cells themselves (Hosoya et al , 2008Zhao et al 2007). In culture conditions, the pulp cells also show the mineralized matrix formation by the induction of various factors (Iohara et al 2006;Nakao et al 2004;Okiji and Yoshiba 2009). These evidences suggest that the dental pulp contains progenitor cells that can differentiate into hard tissueforming cells.…”

Section: Introductionmentioning

confidence: 97%

Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells

Hosoya

Hiraga

Ninomiya

et al. 2012

Histochem Cell Biol

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The cells of the subodontoblastic cell-rich layer in dental pulp are speculated to contain odontoblast progenitor cells because of their positional relationship with odontoblasts as well as their high alkaline phosphatase (ALP) activity. However, it has yet to be determined whether these cells have the ability to differentiate into odontoblastic cells. In the present study, we firstly found that the majority of cells in the subodontoblastic layer expressed Thy-1, a cell-surface marker of stem and progenitor cells. Then, we evaluated the capacity of Thy-1 high- and low-expressing (Thy-1(high) and Thy-1(low)) cells separated from rat dental pulp cells by use of a fluorescence-activated cell sorter to differentiate into hard tissue-forming cells in vitro and in vivo. Following stimulation with bone morphogenetic protein-2, Thy-1(high) cells in vitro showed accelerated induction of ALP activity and formation of alizarin red-positive mineralized matrix compared with Thy-1(low) cells. Furthermore, subcutaneous implantation of Thy-1(high) cells efficiently induced the formation of bone-like matrix. These results collectively suggest that Thy-1-positive dental pulp cells localized in the subodontoblastic layer had the ability to differentiate into hard tissue-forming cells, and thus these cells may serve as a source of odontoblastic cells.

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“…Moreover, Col-I also supports osteogenesis in BMSC and may induce differentiation in the absence of soluble osteoinductive factors (Klees et al 2005;Salasznyk et al 2004). Col-I is also a major organic component of the predentin-dentin matrix (Ruch 1998), and immature adult rat dental pulp cells strongly induces mRNA expression for dentin sialoprotein in Col-I gel cultures (Nakao et al 2004). We have therefore hypothesized that Col-I might facilitate the differentiation of DFC.…”

Section: Introductionmentioning

confidence: 97%

Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

et al. 2007

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We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with beta-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.

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FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells

Cited by 56 publications

References 35 publications

In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells

Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

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