Fetal endothelium dysfunction is associated with circulating maternal levels of sE-selectin, sVCAM1, and sFlt-1 during pre-eclampsia

Veas, Carlos; Aguilera, Valeria; Muñoz, Isabel; Gallardo, Victoria; Miguel, Patricia; González, Marcelo; Lamperti, Liliana; Escudero, Carlos; Aguayo, Claudio

doi:10.3109/14767058.2011.556204

Cited by 40 publications

(40 citation statements)

References 22 publications

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“…In agreement with previous results [47], we found a correlative relationship between NO availability and proliferation in HUVECs in both LOPE and EOPE. This evidence agrees with previous reports where A 2A AR triggers an intracellular pathway dependent on NO synthesis [23,41].…”

Section: Discussionsupporting

confidence: 82%

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Escudero

Bertoglia

Hernadez

et al. 2012

Purinergic Signalling

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To investigate whether fetal endothelial cell proliferation and migration are modulated by the A 2A adenosine receptor (A 2A AR), nitric oxide (NO) and the vascular endothelial growth factor (VEGF) signaling pathway, we isolated human umbilical vein endothelial cells from normal pregnancy (n023), preterm delivery (n04), and late-onset (LOPE, n010) and early-onset preeclampsia (EOPE, n08). We used the non-selective adenosine receptor agonist (NECA) and the selective agonist (CGS-21680) and/or selective antagonist (ZM-241385) for A 2A AR. Also, the nitric oxide synthase (NOS) inhibitor, L-NAME, was used in co-incubation with CGS-21680. Compared to normal pregnancy, EOPE exhibited low cell proliferation and migration associated with reduced expressions of A 2A AR and VEGF and NO synthesis (i.e., total and phosphorylated serine 1177 endothelial NOS and nitrite formation). In contrast, LOPE exhibited the opposite behavior in all these markers compared to normal pregnancy or EOPE. Cell proliferation and migration were increased by CGS-21680 (or NECA) in all analyzed groups (EOPE>LO-PE>normal pregnancy) compared to their respective basal conditions, an effect that was associated with high NO and VEGF synthesis and blocked by ZM-241385 with significantly different IC 50 for each group (EOPE>LOPE>normal pregnancy). The differences seem independent of gestational age.L-NAME blocked the CGS-21680-mediated cell proliferation and migration in normal pregnancy and LOPE (IC 50 036.2± 2.5 and 8.6±2.2 nM, respectively) as well as the VEGF expression in normal pregnancy. Therefore, the A 2A AR/NO/ VEGF signaling pathway exhibits a pro-angiogenic effect in normal pregnancies and LOPE, whereas impairment in this pathway seems related to the reduced angiogenic capacity of the fetal endothelium in EOPE.

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Section: Discussionsupporting

confidence: 82%

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Escudero

Bertoglia

Hernadez

et al. 2012

Purinergic Signalling

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“…Levels of SE-selectin were negatively associated with newborn weight. High circulating levels of maternal endothelial dysfunction markers such as E-selectin present in PE are associated with decreased nitric oxide (NO) synthesis in fetal endothelium and high risk for preterm delivery and very preterm delivery or fetal extremely low birth weight compared with women with low levels (19). Although, Chen study didn't show association between high serum sE-selectin and risk of preterm delivery.…”

Section: J Biol Today's World 2018 Jan; 7 (1): 16-19contrasting

confidence: 47%

“…Today's World. 2018 Jan; 7 (1): [16][17][18][19] lower in case group (P=0.001). Serum concentration of Eselectin was significantly higher in PE compared to normal pregnant women (78.…”

Section: Resultsmentioning

confidence: 99%

Serum E-selectin in Preeclampsia: A Case â Control Study

Ayatollahi¹,

Khadem²,

Kianifar³

et al. 2018

J.JBTW

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Preeclampsia (PE) is an important disease in pregnancy, and is one of the causes of maternal morbidity, mortality and adverse neonatal outcomes. One of the basic pathophysiologies of preeclampsia is endothelial cell dysfunction as suggested by an elevated concentration of endothelin-selectin. The aim of this study was to compare the sera levels of endothelin-selectin (E-selectin) in normal and pre-eclamptic term pregnancy. This case-control study was followed on 80 patients admitted to the Departments of Obstetrics and Gynecology of the Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences. The Control group consisted of 40 normal term pregnant women (≥ 37 weeks) and the Case group was 40 term preeclamptic pregnant women. Participants were enrolled after approval by the ethics committee and received the informed consent. E-selectin concentration evaluated in two groups. The level of E-selectin was significantly in two groups. It was higher in sera of pre-eclamptic patients than the normal pregnancy (p=0.0001). Especially, it was significantly higher in severe pre-eclamptic patients (p<0.05). In conclusion, the found results showed that E-selectin was increased in term of PE that and may be useful for predicting the severity of pre-eclampsia.

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“…The diagnostic sensitivity and specificity in differentiating preeclampsia from gestational and chronic hypertension were 84% and 95%, respectively, for sFlt-1 (59 ). Clinically, sFlt-1 concentrations have been observed to be directly proportional to severity of proteinuria, but inversely correlated with platelet count, gestational age, and neonatal birth weight adjusted for gestational age (60,61 ). In women with preeclampsia, concentrations of sFlt-1 are higher in those with early onset (Ͻ37 weeks), more severe disease (14,28,30,62 ), and SGA (small for gestational age) neonates (28,30,60 ).…”

Section: Angiogenic Factors In the Diagnosis Of Preeclampsiamentioning

confidence: 99%

The Promise of Angiogenic Markers for the Early Diagnosis and Prediction of Preeclampsia

et al. 2012

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BACKGROUND An imbalance in circulating factors that regulate blood vessel formation and health, referred to as angiogenic factors, plays a central role in the pathogenesis of preeclampsia. CONTENT Several studies have demonstrated a strong association between altered circulating angiogenic factors and preeclampsia. These factors include circulating antiangiogenic proteins such as soluble fms-like tyrosine kinase 1 and soluble endoglin and proangiogenic protein such as placental growth factor. Abnormalities in these circulating angiogenic factors are not only present during clinical disease, but also antedate clinical signs and symptoms by several weeks. These alterations are particularly prominent in patients who present with preeclamptic signs and symptoms prematurely and/or in patients with severe preeclampsia. The availability of automated platforms for the rapid measurement of circulating angiogenic proteins in blood samples has now allowed researchers and clinicians to evaluate the utility of these assays in the diagnosis of the disease, in the stratification of patients in clinical trials, or in the monitoring of therapies. In this review we highlight the various studies that have been performed, with a focus on large validation studies. SUMMARY Measurement of circulating angiogenic proteins for the diagnosis and prediction of preeclampsia is still at an early stage but is rapidly evolving. Standardization across the various automated platforms and prospective studies that demonstrate clinical utility are needed.

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Fetal endothelium dysfunction is associated with circulating maternal levels of sE-selectin, sVCAM1, and sFlt-1 during pre-eclampsia

Abstract: High circulating levels of maternal endothelial dysfunction markers present in pre-eclampsia are associated with decreased NO synthesis in fetal endothelium.

Cited by 40 publications

References 22 publications

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Serum E-selectin in Preeclampsia: A Case â Control Study

The Promise of Angiogenic Markers for the Early Diagnosis and Prediction of Preeclampsia

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Fetal endothelium dysfunction is associated with circulating maternal levels of sE-selectin, sVCAM1, and sFlt-1 during pre-eclampsia

Abstract: High circulating levels of maternal endothelial dysfunction markers present in pre-eclampsia are associated with decreased NO synthesis in fetal endothelium.

Cited by 40 publications

References 22 publications

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium during late- and early-onset preeclampsia

Serum E-selectin in Preeclampsia: A Case â Control Study

The Promise of Angiogenic Markers for the Early Diagnosis and Prediction of Preeclampsia

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Serum E-selectin in Preeclampsia: A Case â Control Study