Fertilizing capacity of squid (<i>Todarodes pacificus</i>) spermatozoa collected from various sperm storage sites, with special reference to the role of gelatinous substance from oviducal gland in fertilization and embryonic development

Ikeda, Yuzuru; Sakurai, Yasunori; Shimazaki, Kazunori

doi:10.1080/07924259.1993.9672291

Cited by 35 publications

(25 citation statements)

References 5 publications

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“…It therefore seems that, at the same stages of maturity, the fertilization capacity of spermatozoa from the vas deferens of I. argentinus is higher than that of spermatozoa from the vas deferens of T. pacificus. According to Ikeda et al 10 , spermatozoa in T. pacificus only become mature after they leave the testes and enter the vas deferens. It is thus possible that the maturation process of spermatozoa in the vas deferens may differ between I. argentinus and T. pacificus.…”

Section: Discussionmentioning

confidence: 99%

“…However, mean fertilization rates obtained using spermatozoa from the vas deferens and spermatophores of stage III males were 72.6% and 75.7%, respectively. Ikeda et al 10 reported that the rate of fertilization achieved with spermatozoa (likely to be immotile) from the vas deferens was 30.6-50.5% in T. pacificus, whereas the fertilization rate obtained with spermatozoa from spermatophores was 89.9-99.4%; we assume that the maturity of the males used in their study were at stage V using the scale of Ikeda et al 9 for T. pacificus, or stage IV using the scale for I. argentinus 4 . It therefore seems that, at the same stages of maturity, the fertilization capacity of spermatozoa from the vas deferens of I. argentinus is higher than that of spermatozoa from the vas deferens of T. pacificus.…”

Section: Discussionmentioning

confidence: 99%

“…Several experimental studies on the role of oviducal gland jelly 10,16 demonstrated that if the first expansion or perivitelline space were not developed, neither later expansion nor normal embryonic development followed. Thus, despite the magnitude of chorionic expansion observed at stages 0-10 being very slight, it is a good indicator that subsequent embryonic development will be normal.…”

Section: Discussionmentioning

confidence: 99%

“…A variety of artificial fertilization techniques have recently been established for ommastrephid squids 10,11,16,19 . A common feature of these techniques is not only the methods employed to combine gametes, but also the addition of oviducal gland jelly to the fertilized eggs; the addition of the oviducal gland jelly has been shown to advance chorionic expansion, which is closely related to the normal development of the embryo 16 .…”

Section: Discussionmentioning

confidence: 99%

“…While I. argentinus are known to store spermatozoa in the genital organs, vas deferens, spermatophores, and testis 21 , the viability of the spermatozoa from these tissues is unknown. In T. pacificus, lower fertilization abil- ity has been observed using spermatozoa from the vas deferens and no ability has been observed using spermatozoa from the testis 8,10 . Since the possibility of sourcing male gametes from different organs of freshly caught squid would likely increase the opportunity of artificial fertilization for offshore species such as I. argentinus, developing methods for the prolonged storage of viable gametes would mean that experiments using offshore species could be conducted in land-based laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Useful Techniques for Artificial Fertilization of the Ommastrephid Squid Illex argentinus

Sakai¹,

Brunetti²,

Ivanovic³

et al. 2011

JARQ

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This study examined the rate of fertilization in the Argentine shortfin squid Illex argentinus after artificial insemination using sperm from different sources, as well as the effect of cold-storage on the fertilization capacity of gametes from mature squids, and the impact of mechanical agitation on embryonic development. In addition, the process of chorionic expansion during the course of embryonic development was also examined. High fertilization rates were obtained from spermatophores (76-95%) and spermatozoa from the vas deferens (73-87%), but no fertilization was observed using spermatozoa from testes. Female and male gametes stored at 0°C had a high fertilization capacity (>72%) within 24 h. Embryos (stages 4 to 17; 3 to 61 h at 20 o C) subjected to mechanical agitation at a critical period (stages 5 to 12; 5 to 20 h at 20 o C) exhibited high mortality and abnormal development. In the course of embryonic development, the chorion expanded four times: once at fertilization and then at developmental stages 15-16, 17-21, and finally at stages 25-27. The growth rate of the third expansion corresponded with an increase in embryonic body length. To improve the fertilization rate and increase the production of healthy paralarvae in on-board experiments, factors such as the origin of spermatozoa, gamete storage methods, and the handling of fertilized eggs during the critical period should be carefully controlled.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Useful Techniques for Artificial Fertilization of the Ommastrephid Squid Illex argentinus

Sakai¹,

Brunetti²,

Ivanovic³

et al. 2011

JARQ

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Sex steroid hormone fluctuations and morphological changes of the reproductive system of the female ofOctopus vulgaris throughout the annual cycle

2001

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Sex steroids (17β‐estradiol and progesterone) and morphological variations of the reproductive system of the female of Octopus vulgaris from the Bay of Naples were followed over a period of 2 years. The increase in the ovary weight was independent of body weight as demonstrated by the gonado‐somatic index (GSI). Both 17β‐estradiol and progesterone have been detected in the ovary of O. vulgaris, and their concentrations changed in correlation with the ovarian development. No 17β‐estradiol or progesterone was found in the hemolymph. 3β‐Hydroxysteroid dehydrogenase activity has been detected in the ovary, indicating that in the female of O. vulgaris the reproductive system is a source of sex steroid hormones. According to the morphological changes of the ovary, the ovarian cycle can be divided into the following phases: previtellogenesis; early vitellogenesis, full vitellogenesis and late vitellogenesis. The morphological changes of the oviducts and oviducal glands throughout the reproductive cycle were in accordance with their role in the transport and secretion of gelatinous coat covering the eggs, as well as in sperm storage and sperm reactivation during fertilization. J. Exp. Zool. 289:33–47, 2001. © 2001 Wiley‐Liss, Inc.

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Cephalopoda

Holst,

Howard,

Crook

2024

The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals

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Fertilizing capacity of squid (Todarodes pacificus) spermatozoa collected from various sperm storage sites, with special reference to the role of gelatinous substance from oviducal gland in fertilization and embryonic development

Cited by 35 publications

References 5 publications

Useful Techniques for Artificial Fertilization of the Ommastrephid Squid Illex argentinus

Useful Techniques for Artificial Fertilization of the Ommastrephid Squid Illex argentinus

Sex steroid hormone fluctuations and morphological changes of the reproductive system of the female ofOctopus vulgaris throughout the annual cycle

Cephalopoda

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