A joint research cruise (Japan-Argentina-Uruguay) was carried out in the South-western Atlantic during August-September 1989 in order to study the winter-spawning and hatchery areas ofIllex argentinus, and also the migration pattern of the juveniles towards the continental shelf. A few Rhynchoteuthion larvae were found in subtropical waters of the Brazil Current, next to the Brazil-Malvinas confluence, and in the frontal zone with shelf water, but never at temperatures below 14°C. Large numbers of juveniles found in subantarctic waters (6-10°C) on the shelf were probably migrating southward from their hatchery grounds following the zooplankton concentrations on which they were feeding.

The diet and feeding behaviour of Illex argentinus were determined from analysis of stomach contents of squid caught during four bottom trawl surveys carried out over the Bonaerensis (34–40°S, autumn–winter 1991) and Patagonian (45–55°S, summer–autumn 1992) shelves of Argentina. Crustaceans, mainly Themisto gaudichaudii and, to a lesser extent, euphausiids were the most important components of the diet in both areas and years, although there were considerable geographical differences in the relative proportion of the main prey. In the Patagonian area, during summer and autumn of 1992, almost all squid preyed on crustaceans (percentage occurrence = 85.29%), followed by squid (11.76%) and fish (2.94%). On the Bonaerensis shelf, during autumn and winter of 1991, crustaceans were most important by percentage occurrence (56.96%), but fish and squid accounted for a large proportion of the diet (29.41% and 13.62% respectively). Myctophids were prevalent among the fish and cannibalism was on I. argentinus juveniles. Changes in diet composition with squid size demonstrated the opportunistic nature of I. argentinus feeding. Although large squids were able to catch large prey (fish, squid), they also fed on small crustaceans when available, especially in the Patagonian area. The relative abundance of different prey organisms probably determined the diet. In both areas and both years I. argentinus fed mostly during daylight hours, starting in the morning and reaching a maximum in the afternoon. No difference in feeding behaviour was observed between the sexes.

To identify sub-daily or aperiodic increments of statolith growth in the ommastrephid squid Illex argentinus, we examined statolith microstructure, especially with regard to the natal ring, where counting of daily growth increments should begin, and the widths of subsequent daily increments. Paralarvae obtained by artificial fertilisation were incubated on board at different temperatures ranging from 11.4 to 25.4°C, and were starved throughout the experiments. We observed statolith growth from newly hatched to 10-day-old paralarvae and used alizarine complexone staining to attempt validation of the growth. The maximum statolith radius (MSR) of newly hatched paralarvae was constant at 21.1 μm across the full range of temperatures, with the exception of 25.4°C. Daily growth of MSR was analysed separately in two phases, the pre-yolk-absorption phase (i.e. yolk sac still present) and the post-yolk-absorption phase. During the pre-yolk-absorption phase, the daily growth rate (DGR, y) of the MSR varied from 3 to 7 μm day–1 depending on rearing temperature (x) and was expressed as y = 0.37x – 1.77. We concluded that the natal ring forms at 21 μm MSR. The initial increment width obtained from the DGR of MSR seems applicable for distinguishing daily rings from sub-daily rings, although this application should be limited to hatchling paralarvae in the pre-yolk-absorption phase.

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Artificial insemination of the Argentine shortfin squid Illex argentinus was carried out on board, and the hatchlings were successfully obtained. Applying oviducal gland jelly to inseminated egg al lowed the embryo to expand chorion and develop perivitelline space, but applying nidamental gland jel ly and applying no jelly was not successful. The eggs which developed perivitelline space only continued embryonic development normally and proceeded onto hatching. The embryonic development was ob served in vitro throughout the experiment. The hatchlings survived for about one week in vitro without feeding.