Fertilization of Denuded Rabbit Eggs in vitro by Sperm recovered from the Uterus or Vagina

Fertilization in Vitro of Mouse and Hamster Eggs After the Removal of Follicular Cells

“…This confirms the statement by Yanagimachi (1969) (Chang et al, 1971 ;Fraser et al, 1971) and in the hamster (the present study), while the presence of follicular cells appears to facilitate the penetration of mouse eggs (Cross & Brinster, 1970;present study), the possibility of a species difference concerning the rôle of follicular cells for fertilization cannot be excluded. This work was supported by grants from the U.S. Public Health Service (GM 14370) and the Ford Foundation.…”

“…Recently, it was reported that removal of the cumulus oophorus and the corona radiata from rabbit eggs does not lower the proportion of eggs fertilized in vitro (Chang, Hanada & Hunt, 1971 ;Fraser, Dandekar & Vaidya, 1971). By contrast, Cross & Brinster (1970) reported that the removal of the follicular cells from the mouse eggs reduced the fertilization rate inseminated with spermatozoa recovered from the uterus.…”

New techniques, applications and perspectives in neuropeptide research

“…However, gaining this information can be challenging and cumbersome. While it has been almost a century since the first neuropeptide, substance P, was discovered (von Euler and Gaddum, 1931), and nearly 50 years since the sequence of that peptide was determined (Chang et al, 1971), technology has since developed impressively, and there are now records of almost 6000 neuropeptide sequences across all species .…”

Environment and medium volume influence in vitro fertilisation of pig oocytes

“…The effect of the presence of cumulus cells on the results of IVF varies among species. In the rabbit and hamster cumulus cell removal had no effect on the proportion of eggs fertilised in vitro (Chang et al, 1971;Fraser et al, 1971;Miyamoto & Chang, 1972). Removal of cumulus cells reduced the fertilisation rate of mouse eggs (Cross & Brinster, 1970;Miyamoto & Chang, 1972).…”