Fertilization in Vitro of Mouse and Hamster Eggs After the Removal of Follicular Cells

Abstract

Summary. The removal of follicular cells decreased the proportion of mouse eggs penetrated by epididymal spermatozoa, but did not affect the proportion of hamster eggs penetrated, whether the spermatozoa were taken from the epididymis or from the uterus.

Presence of follicular fluid in the porcine oviduct and its contribution to the acrosome reaction

“…The Gordon HYDothesis (Gordon, 1973: Gordon et al, 1978 Gordon hypothesizes that the removal of a seminal plasma factor activates a calcium independent ATPase in the sperrn plasma membrane. This ATPase then transports extracellular Ca 2 + into the space between the outer acrosomal and plasma Miyamoto and Chang, 1972). In addition, as was mentioned earlier, Moore and Bedford (1978) have reported that fertilization of washed ova can occur in vivo and in vitro in the absence of follicular products.…”

In-vitro fertilization of hamster eggs in different media and the stimulating effect of heterologous and homologous spermatozoa

“…The role of cumulus components for the capacitation of hamster spermatozoa and penetration of hamster eggs has been reported by Gwatkin et al (1972), but Miyamoto & Chang (1972) observed that there was no difference in the penetration rates (91 versus 87 %) of hamster eggs with or without follicular cells incubated with epididymal spermatozoa in an equal volume of Tyrode's solution and heated hamster blood serum. Since only 4% of denuded hamster eggs were penetrated in the rabbit medium which contained BSA (Table 2), the heated hamster blood serum may play an important role for the capacitation and acrosome reaction of hamster spermatozoa in the absence of follicular cells.…”

“…or for the rabbit (Brackett, 1970) showed that rabbit medium was superior to the rat medium but that the penetration rates were still quite low (12-36%, Table 1). Although hamster eggs can be fertilized in a modified Tyrode's solution containing BSA without any biological fluid (Bavister, 1969(Bavister, , 1973, high penetration rates occur in Tyrode's solution containing follicular fluids (Yana¬ gimachi, 1969a, b), or blood serum (Yanagimachi, 1970;Miyamoto & Chang, 1972,1973Miyamoto et al, 1974). It appears, therefore, that a particular medium is best for a given species and that a medium with biological fluid may be better than one without.…”

“…Since the first achievement of in-vitro fertilization of hamster eggs by epididymal spermatozoa (Yanagimachi & Chang, 1963, 1964, in-vitro capacitation of spermatozoa and fertilization of hamster eggs in the presence of follicular fluid (Barros & Austin, 1967;Yanagimachi, 1969a, b;Gwatkin & Anderson, 1969) or of blood serum (Yanagimachi, 1970;Barros & Garavagno, 1970;Miyamoto & Chang, 1972, 1973 have been described. Sperm capacitation and fertilization of hamster eggs in vitro can be achieved in a chemically defined medium without addition of any bio¬ logical fluids (Bavister, 1969), although the acrosome reaction was "only induced in the majority of spermatozoa following the addition of the products of ovulation" (Bavister, 1973).…”

Fertilization in vitro of hamster and mouse eggs in a chemically defined medium

“…Although it was reported by Hanada & Chang (1976) (Brackett, 1970) or rat eggs (Toyoda & Chang, 1974), much higher penetration rates occur in Tyrode's solution and modified Medium 199 containing follicular fluids (Barros & Austin, 1967;Yanagimachi, 1969a, b;Gwatkin & Andersen, 1969) or blood serum (Yanagimachi, 1970;Miyamoto & Chang, 1972b). Gwatkin, Andersen & Hutchinson (1972) reported that the cumulus components play an important role in the capacitation of spermatozoa and fertilization of hamster eggs in vitro.…”