Fertilization of cumulus‐free, zona‐intact mouse ova in vitro at high and low sperm concentrations

Stanger, James; Quinn, Patrick

doi:10.1002/mrd.1120050107

Cited by 25 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lower cleavage rates and development with CDOs in the present study confirm previous results with oocytes from mice, cattle, and humans [39][40][41][42][43], while other researchers observed no significant differences in fertilization using murine CDOs or CEOs [44,45]. It was reported that cumulus cells improve the fertilization rate by providing a capacitationinducing mechanism [40,42] or increase the contact area between spermatozoa and the oocyte, thus allowing more spermatozoa to become attached [41,42]. In addition, they may assist acrosome-reacting or acrosome-reacted spermatozoa in penetrating the ZP, as oocyte movement is greatly reduced by the cumulus cells.…”

Section: Discussionsupporting

confidence: 95%

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Mahabir¹,

Bulian²,

Needham³

et al. 2009

Biology of Reproduction

View full text Add to dashboard Cite

The risk of transmission of mouse minute virus (MMV) to recipients of murine embryos arising from in vitro fertilization (IVF) of cumulus-enclosed oocytes (CEOs) or without cumulus cells (CDOs) in the presence of MMV-exposed (10(4) TCID(50) [mean tissue culture infective dose]/ml MMVp [prototype strain of MMV]) spermatozoa was evaluated. Also, the time after embryo transfer to detection of MMV antibody and the presence of MMV DNA in the mesenteric lymph nodes of recipients and pups were investigated. All mice were MMV free, but two seropositive recipients and four seropositive pups were found in the group with CDOs. With regard to the CEOs, two of 11 holding drops and five of 11 groups of embryos were MMV positive using PCR, while neither holding drops nor embryos carried infectious MMVp, as evidenced by the in vitro infectivity assay. From IVF with CDOs, five of 14 holding drops and four of nine groups of embryos were MMV positive, while one of 14 holding drops and no embryos carried infectious MMVp. When 10(5) cumulus cells were analyzed 5 h after exposure to 10(4) TCID(50)/ml MMVp, cells had an average titer of 10(4) TCID(50)/ml MMVp. The present data show that, in contrast to CDOs, 2-cell embryos from CEOs did not transmit infectious MMVp to the holding drops and to recipients. This observation is due to the presence of cumulus cells during the IVF process that reduce entry of MMV into the zona pellucida and absorb some of the virus. These data further confirm the efficacy of the IVF procedure in producing embryos that are free of infectious virus, leading to virus-free seronegative recipients and rederived pups.

show abstract

Section: Discussionsupporting

confidence: 95%

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Mahabir¹,

Bulian²,

Needham³

et al. 2009

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…This approach was claimed to show a substantial improvement in the sperm/oocyte ratio without increasing the incidence of polyspermia (Gordon & Talanski, 1986). The mechanical dis¬ ruption of the zona pellucida by cutting with a microneedle has been utilized for nuclear manipulation (Tsunoda et al, 1986) (Stanger & Quinn, 1982).…”

Section: Introductionmentioning

confidence: 99%

Comparison of zona cutting and zona drilling as techniques for assisted fertilization in the mouse

Depypere

McLaughlin²,

Seamark³

et al. 1988

Reproduction

View full text Add to dashboard Cite

show abstract

Effect of polyamines on fertilization of mouse ova in vitro

Stanger¹,

Quinn²

1982

J. Exp. Zool.

View full text Add to dashboard Cite

After a 1-hour preincubation of epididymal mouse spermatozoa at a concentration of 2 X 10(6)/ml in 0.23 mM spermine, the proportion of F1(C57BL X CBA) mouse ova fertilized after 1 and 2 hours was significantly greater than with untreated spermatozoa. Spermine also significantly increased the proportion of ova fertilized at the sub optimal sperm concentration of 2 X 10(5)/ml. The stimulatory effect was lost when the protein source in the fertilization medium was changed from human serum albumin V (HSA) to HSA crystalline. This provides indirect evidence that albumin is directly involved in the capacitation process and that the crystalline is more potent than the fraction V preparation. At equimolar concentrations, spermidine was partially and putrescine was totally inhibitory to fertilization. Mechanisms whereby spermine may affect metabolic activity or sperm-zona binding are discussed. It is suggested spermine may also be present in ovulatory fluid and therefore could potentially be involved in fertilization in vivo.

show abstract

Fertilization of cumulus‐free, zona‐intact mouse ova in vitro at high and low sperm concentrations

Cited by 25 publications

References 29 publications

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Comparison of zona cutting and zona drilling as techniques for assisted fertilization in the mouse

Effect of polyamines on fertilization of mouse ova in vitro

Contact Info

Product

Resources

About