Effect of polyamines on fertilization of mouse ova in vitro

Stanger, James; Quinn, P. J.

doi:10.1002/jez.1402200313

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…Spermine is abundant in semen [13, 17] and critical to male fertility [42]. It is possible that spermine is also critical to male fertility and sperm mobility in sea lamprey, as has been shown in mammals [16, 43]. An ovulatory female lamprey should be keenly attuned to such a signal that is a reliable indicator for sperm availability to maximize the fertilization rate and her fitness [44].…”

Section: Discussionmentioning

confidence: 99%

Spermine in semen of male sea lamprey acts as a sex pheromone

et al. 2019

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Semen is fundamental for sexual reproduction. The non-sperm part of ejaculated semen, or seminal plasma, facilitates the delivery of sperm to the eggs. The seminal plasma of some species with internal fertilization contains anti-aphrodisiac molecules that deter promiscuity in post-copulatory females, conferring fitness benefits to the ejaculating male. By contrast, in some taxa with external fertilization such as fish, exposure to semen promotes spawning behaviors. However, no specific compounds in semen have been identified as aphrodisiac pheromones. We sought to identify a pheromone from the milt (fish semen) of sea lamprey ( Petromyzon marinus ), a jawless fish that spawns in lek-like aggregations in which each spermiating male defends a nest, and ovulatory females move from nest to nest to mate. We postulated that milt compounds signal to ovulatory females the presence of spawning spermiating males. We determined that spermine, an odorous polyamine initially identified from human semen, is indeed a milt pheromone. At concentrations as low as 10 −14 molar, spermine stimulated the lamprey olfactory system and attracted ovulatory females but did not attract males or pre-ovulatory females. We found spermine activated a trace amine-associated receptor (TAAR)-like receptor in the lamprey olfactory epithelium. A novel antagonist to that receptor nullified the attraction of ovulatory females to spermine. Our results elucidate a mechanism whereby a seminal plasma pheromone attracts ready-to-mate females and implicates a possible conservation of the olfactory detection of semen from jawless vertebrates to humans. Milt pheromones may also have management implications for sea lamprey populations.

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Section: Discussionmentioning

confidence: 99%

Spermine in semen of male sea lamprey acts as a sex pheromone

et al. 2019

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“…In the second series, a range of concentration of propranolol(l0-50 pM) were tested at 2 x lo6 sperm/ml. However, because ovum penetration occurs more quickly at this density [Stanger and Quinn, 1982b], ova were incubated with spermatozoa for only 2 hr. The acrosome status of propranolol-treated spermatozoa was estimated by acridine orange fluorescence (0.001 070 w/v in FM/15).…”

Section: Methodsmentioning

confidence: 99%

The effect of catecholamines and their antagonists on the fertilization of cumulus‐free mouse ova in vitro at a suboptimal spermatozoal density

Stanger

1983

Gamete Research

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A suboptimal sperm concentration was used to assess the capacity of catecholamines to stimulate the fertilization of cumulus free F1,(C57BL × CBA) mouse ova in vitro. At a concentration of 50 μM, (L) epinephrine significantly increased the proportion of ova fertilized at 2 × l05 spermatozoa/ml. However, when (D, L) propranolol at an equimolar concentration was tested for inhibition of the (L) epinephrine effect, fertilization was inhibited in both the test and control dishes. At l0μM, propanolol by itself or in the presence of 50μM (L) epinephrine significantly increased the number of ova fertilized at 2 × l05 sperm/ml. Norepinephrine (50 μM) and phentolamine (50 μM), either alone or together, were also slightly stimulatory. Some data are presented to suggest that propranolol may act in a nonadrenergic manner to precipitate the acrosome reaction and that the stimulatory effect is maximised when it is added to spermatozoa at the same time as ova addition. It was suggested that propranolol may act to trigger calcium influx by a nonspecific alteration in membrane function for example in (Ca + Mg) ATPase activity. It was concluded that spermatozoa at suboptimal densities are capable of achieving fertilization and that sperm concentration dependency in fertilization in vitro may be a reflection of the proportion of spermatozoa achieving capacitation.

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Effects of polyamines upon capacitation and fertilization in the guinea pig

Fleming

Armstrong

1985

J. Exp. Zool.

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The effects of polyamines upon the ability of guinea pig spermatozoa to undergo the acrosome reaction and upon sperm-egg fusion were investigated. All of the polyamines (polyarginines and compound 48/80) stimulated the occurrence of the acrosome reaction, although differences in the duration of the preincubation period required were noted. The stimulatory effect of the polyarginines could not be reversed by washing, while that of 48/80 was abolished by its removal prior to induction of the acrosome reaction. Polyarginine-treated spermatozoa were fully capable of fertilizing intact guinea pig eggs. In contrast, 48/80 completely blocked sperm-egg fusion at only 5-10 micrograms/ml. This inhibition was reversible by washing either treated gamete. The ability of polyamines which may also be present at the time and site of fertilization, in vivo, to inhibit or stimulate gamete functions or interactions is discussed.

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Effect of polyamines on fertilization of mouse ova in vitro

Cited by 7 publications

References 23 publications

Spermine in semen of male sea lamprey acts as a sex pheromone

Spermine in semen of male sea lamprey acts as a sex pheromone

The effect of catecholamines and their antagonists on the fertilization of cumulus‐free mouse ova in vitro at a suboptimal spermatozoal density

Effects of polyamines upon capacitation and fertilization in the guinea pig

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