Fertility of cryopreserved spermatozoa of the Japanese pearl oyster, Pinctada fucata martensii

Narita, Teruyoshi; Kawamoto, Takayuki; Isowa, Kiyoshi; Aoki, Hideo; Hayashi, Masahiro; Komaru, Akira; Ohta, Hiromi

doi:10.1016/j.aquaculture.2008.01.009

Cited by 16 publications

(16 citation statements)

References 20 publications

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“…To clarify this issue, we observed the structure of spermatozoa just after dilution with cryopreservation diluent before cooling, and the structure of normal acrosome reaction induced by egg–sea water. Consequently, the noticeable decrease in fertilization rates in cryopreserved spermatozoa of the pearl oyster P. fucata martensii 5 could be derived from the extrusion of the contents of acrosomal vesicles due to the eversion of acrosomal vesicles through the cryopreservation procedure.…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported an effective cryopreservation method of spermatozoa that obtained a maximal post‐thaw motility 4 and fertilization rate 5 in the Japanese pearl oyster. The motility of cryopreserved spermatozoa was approximately 30% of that of fresh spermatozoa 4 .…”

Section: Introductionmentioning

confidence: 99%

“…In artificial fertilization experiments, approximately 10 times the volume of cryopreserved spermatozoa is required to obtain equivalent fertilization rates by fresh spermatozoa. (Approximately 60% fertilization rates could be obtained by adding 3.5 × 10 7 cryopreserved spermatozoa/mL to 1.0 × 10 4 eggs/mL) 5 . The cause of the inferior motility and fertility of cryopreserved spermatozoa must be elucidated to establish more effective cryopreservation methods.…”

Section: Introductionmentioning

confidence: 99%

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Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

et al. 2008

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To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular spermatozoa were diluted with cryopreservation diluent (10% methanol + 18% fetal bovine serum + 72% sea water), and dispensed into 0.25-mL straws. The straws were cooled at a rate of approximately -20°C/min to -50°C, and subsequently immersed in liquid nitrogen. Percentage motility of spermatozoa before cryopreservation was 69.9 Ϯ 4.2%, and that of cryopreserved spermatozoa was 24.0 Ϯ 1.8%, respectively. In cryopreserved spermatozoa, the percentage that lacked or had a deformed flagellum was 56.6 Ϯ 3.9%, while in fresh spermatozoa this was 8.7 Ϯ 2.0%. In cryopreserved spermatozoa, the percentage of deformed acrosomes was 76.6 Ϯ 5.2%, while in fresh spermatozoa this was only 0.9 Ϯ 0.3%. Cryopreserved spermatozoa with a normal acrosome and flagellum were only 15.4 Ϯ 3.5% of those in fresh spermatozoa. These results indicate that lesion of the flagellum and deformation of the acrosome occurred through the cryopreservation procedure, and both types of damage lead to loss of the motility and fertility in thawed spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

et al. 2008

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“…In Pacific oyster, maximum fertilization rates of thawed sperm were noted for ratios ranging from 1000 to 10 000 (Adams et al 2008). In the Japanese pearl oyster, the decrease in the fertilization capacity of thawed sperm can be compensated by increasing sperm to egg ratio to make it 10 times higher than that used for fresh sperm (Narita et al 2008). The lower fertilization capacity of black-lip pearl oyster thawed sperm compared with fresh sperm may reflect the 25% decrease in sperm motility and 23% decrease in sperm viability.…”

Section: Discussionmentioning

confidence: 99%

“…Ammonia treatment can be used to compensate for this imbalance, as the in vitro test of Wada (1963) confirms that the oocyte maturation process can be obtained artificially by stripping the gonad and treating the oocytes with ammonia, as performed on the Japanese pearl oyster P. fucata (Narita et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Hui

Vonau

Moriceau

et al. 2011

Aquat. Living Resour.

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-Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me 2 SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23% lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80% oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.

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Sperm cryopreservation in marine mollusk: a review

Liu

Robinson

et al. 2015

Aquacult Int

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Fertility of cryopreserved spermatozoa of the Japanese pearl oyster, Pinctada fucata martensii

Cited by 16 publications

References 20 publications

Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Sperm cryopreservation in marine mollusk: a review

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