Sperm cryopreservation in marine mollusk: a review

Liu, Yibing; Li, Xiaoxu; Robinson, Nick; Qin, Jianguang

doi:10.1007/s10499-015-9900-0

Cited by 30 publications

(48 citation statements)

References 63 publications

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“…In this study, the high total sperm motility after thawing was obtained at 5%–20% DMSO, as well as fertilization rate. The results reported in this investigation was consistent with other studies that concentrations from 5% to 10% were found suitable for oyster, pearl oyster and sea urchin specimens (Hassan et al, ; Liu et al, ; Paredes, ).…”

Section: Discussionsupporting

confidence: 93%

“…The chosen cryoprotectant agent, equilibration time and cooling rate are considered to be the most influential factors contributing to successful preservation (Acosta‐Salmón, Jerry, & Southgate, ; Dong, Huang, Eudeline, Allen, & Tiersch, ; Dong, Huang, Eudeline, & Tiersch, ). In this study, the calcium‐free Hanks’ balanced salt solution was used as the extender for its ability to inhibit sperm activation and often used in marine molluscs with strip spawning (Liu et al, ). In this study, to select optimal CPA for Chlamys nobilis sperm cryopreservation, four common permeant cryoprotectants (DMSO, MET, PG and EG) were used, and DMSO treatment groups obtained the highest motility followed by MET.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, DMSO is recognized as the most successful CPA out of many CPAs tested in various studies published on marine species (Liu et al, ; Paredes, ), because it offers the highest post‐freeze survival reputation. In this study, the results indicated that the cryoprotectant DMSO may be more suitable for Chlamys nobilis sperm programmable cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…Sperm cryopreservation is a valuable technique, using liquid nitrogen (LN) as a coolant for long-term storage. Cryopreservation can enhance the efficiency of genetic improvement programs, commercial production and endangered species conservation (Liu, Li, Robinson, & Qin, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

et al. 2019

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A study on Chlamys nobilis sperm cryopreservation by a programmable freezing method was conducted under laboratory condition. Four cryoprotectant agents (dimethyl sulfoxide [DMSO], methanol [MET], propanediol[PG] and ethylene glycol [EG]) and four concentrations (5%, 10%, 20% and 30%) were evaluated for their ability to retain sperm motility, movement characteristics and fertility. Results showed that cryopreserved sperm total motility produced by DMSO and MET at 5%, 10% and 20% were higher than other cryoprotectant treatment groups (CPA groups), as well as rapid sperm percentage. The curvilinear (VCL) and straight line (VSL) velocity produced by DMSO at 5% significantly higher than other CPA groups (p < 0.05), while no significant differences were found for average path (VAP) velocity. The lateral head displacement (ALH) in all CPA groups was similar and without significant difference (p > 0.05), as well as the beat‐cross frequency (BCF). A significant higher fertilization rate was produced in DMSO than that in MET at same concentration (p < 0.05), and no significant differences were found for differing concentrations of the same cryoprotectant (p > 0.05). Overall, 5%‐20% DMSO was more suitable for Chlamys nobilis sperm programmable cryopreservation when the calcium‐free Hanks’ balanced salt solution was used as the extender, and 10°C/min from 0°C to −80°C was used as freezing rate. The findings presented in this study will benefit conservation programs for Chlamys nobilis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

et al. 2019

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“…The technique of sperm cryopreservation is used for breeding in aquaculture species and for maintaining genetic diversity of seedlings (Liu, Li, Robinson, & Qin, ; McAndrew, Rana, & Penman, ). During cryopreservation, cells are exposed to various deleterious physical effects (Chao & Liao, ; Leung, ; Rana, ).…”

Section: Introductionmentioning

confidence: 99%

Motility and fertility of cryopreserved spermatozoa of the Japanese sea cucumber Apostichopus japonicus

et al. 2018

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We developed both a cryopreservation method for Japanese sea cucumber spermatozoa and an artificial fertilization method using post-thaw spermatozoa. Twenty per cent dimethyl sulfoxide (DMSO), 16% foetal bovine serum, and 64% artificial seawater were suitable cryodiluent, and the diluent was pre-cooled to 0°C. Semen was diluted with the solution and enclosed in a 250 μl straw, cooled to −50°C at 10.4 ± 0.4°C/ min, and immediately immersed in liquid nitrogen. Although this method showed the highest post-thaw motility in all the conditions we examined, its post-thaw motility was still less than approximately 15%. Artificial fertilization was carried out by adding post-thaw semen with a cryodiluent to the oocytes. The fertilization rate of 200 oocytes/ml seawater increased with the amount of post-thaw semen from 1 to 5 μl but showed a significant decrease at 25 μl. This decrease was considered to be due to DMSO in the cryodiluent, because the fertilization rate of the fresh semen decreased sharply when the DMSO concentration around the oocytes was 1.0% or more. Further improvement in increasing post-thaw motility and lowering the cryoprotectant concentration is necessary for commercial-scale artificial fertilization.

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