Fertility of Chickens Inseminated Intraperitoneally with Semen Preserved in Liquid Nitrogen

Avian Physiology

“…Tanaka and Wilcox (1966) have also studied the effects of different hypertonic solutions on sperm motility. Highest fertility (77%) was obtained in semen in a special diluent that was cooled at 6°C/min (Harris, 1968). Highest fertility (77%) was obtained in semen in a special diluent that was cooled at 6°C/min (Harris, 1968).…”

Changes in the Ultrastructure of the Fowl Spermatozoon Due to Rapid Freeze--Thaw

“…Full motility could be attained when fowl spermatozoa were frozen in a glycerol diluent but techniques involving the use of 5 to 20% glycerol levels had an adverse effect on sperm fertility (Smith & Polge, 1950). Removal of the glycerol before deep insemination (Polge, 1951;Clark & Shaffner, 1960) or the substitution of dimethylsulphoxide and polyvinylpyrrolidone for glycerol as the cryopreservative (Harris, 1968) (Harris, 1968), but shallow intravaginal insemination reduced the fertility level. Additional evi¬ dence suggests that the depth of insemination may possibly influence the level of fertility obtained with fowl spermatozoa after freezing.…”

“…Additional evi¬ dence suggests that the depth of insemination may possibly influence the level of fertility obtained with fowl spermatozoa after freezing. Higher fertility occurred when semen was deposited deep into the oviduct by the intrauterine (Allen, 1958;Clark & Shaffner, 1960; or the intraperitoneal (Brown, Harris & Hobbs, 1963;Harris, 1968) insemination tech¬ niques.…”