Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model

Wang, Zhen; Li, Hongbo; Zhou, Wei; Lee, Jintae; Liu, Zhenbin; An, Zhixing; Xu, Dan; Mo, Haizhen; Hu, Liangbin; Zhou, Xiaohui

doi:10.1016/j.biomaterials.2022.121842

Cited by 24 publications

(17 citation statements)

References 72 publications

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“…The influx of Fe ions reacts with H 2 O 2 from bacterial metabolic products, and ROS accumulate, resulting in lipid peroxidation . Glutathione can remove ROS and inhibit lipid peroxidation, while ferroptosis-inducing factors accumulate ROS and lipid peroxidation affecting glutathione peroxidase . Although cells and bacteria are different in structures (e.g., cell membrane, organelles, and nucleus), which induce different ferroptosis regulatory pathways, they have the similar hallmarks of ferroptosis, including GSH consumption, lipid peroxidation, and inhibited respiratory chain.…”

Section: Resultsmentioning

confidence: 99%

Alkaline “Nanoswords” Coordinate Ferroptosis-like Bacterial Death for Antibiosis and Osseointegration

et al. 2023

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Ferroptosis is an iron-dependent cell death and is associated with cancer therapy. Can it play a role in resistance of postoperative infection of implants, especially with an extracellular supplement of Fe ions in a non-cytotoxic dose? To answer this, "nanoswords" of Fe-doped titanite are fabricated on a Ti implant surface to resist bacterial invasion by a synergistic action of ferroptosis-like bacteria killing, proton disturbance, and physical puncture. The related antibiosis mechanism is explored by atomic force microscopy and genome sequencing. The nanoswords induce an increased local pH value, which not only weakens the proton motive force, reducing adenosine triphosphate synthesis of Staphylococcus aureus, but also decreases the membrane modulus, making the nanoswords distort and even puncture a bacterial membrane easily. Simultaneously, more Fe ions are taken by bacteria due to increased bacterial membrane permeability, resulting in ferroptosis-like death of bacteria, and this is demonstrated by intracellular iron enrichment, lipid peroxidation, and glutathione depletion. Interestingly, a microenvironment constructed by these nanoswords improves osteoblast behavior in vitro and bone regeneration in vivo. Overall, the nanoswords can induce ferroptosis-like bacterial death without cytotoxicity and have great promise in applications with clinical implants for outstanding antibiosis and biointegration performance.

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Section: Resultsmentioning

confidence: 99%

Alkaline “Nanoswords” Coordinate Ferroptosis-like Bacterial Death for Antibiosis and Osseointegration

et al. 2023

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“…Studies have shown that delivering metallic iron into infected tissue can induce a ferroptosis-like response in pathogenic bacteria. [31][32][33] However, the therapeutic efficiency based on iron-based nanomaterials is currently unsatisfactory. More recently, Sun et al 34…”

Section: Other Noble Single Atom Nanozymesmentioning

confidence: 99%

Single atom nanozymes for bacterial infection therapy

Lan,

Chen,

et al. 2024

Biomater. Sci.

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“…122 Instead of loading phosphorus, Wang and co-workers prepared a FeSO 4loaded hydrogel by electrostatically cross-linking negatively charged hyaluronic acid (HA) with positively charged Fe 2+ in the presence of ascorbic acid (ASC). 123 The hydrogel exhibited excellent self-healing properties since it is composed of dynamic bonds. ASC was introduced to prevent Fe 2+ oxidation and retain its ROS-eruption and lipid-peroxidation abilities, which trigger ferroptosis toward MRSA.…”

Section: Protein and Bacteriophagementioning

confidence: 99%

“…Therefore, wound healing requires stimulating cell proliferation, while eradicating bacteria for infection control in diabetes patients . Instead of loading phosphorus, Wang and co-workers prepared a FeSO 4 -loaded hydrogel by electrostatically cross-linking negatively charged hyaluronic acid (HA) with positively charged Fe 2+ in the presence of ascorbic acid (ASC) . The hydrogel exhibited excellent self-healing properties since it is composed of dynamic bonds.…”

Section: Hydrogel-based Strategy For Osteomyelitis Treatmentmentioning

confidence: 99%

Hydrogel-Based Strategies for the Management of Osteomyelitis

Kuo

Lin

Yeh

2023

ACS Biomater. Sci. Eng.

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Osteomyelitis is a type of bone infection caused by bacteria, with Staphylococcus sepsis being responsible for most cases. Osteomyelitis treatment generally requires a multifaceted approach that may include intervention of surgery and administration of antibacterial agents, where several materials have been utilized as delivery vehicles for antibiotics and other antibacterial materials. Hydrogel has become a popular candidate for osteomyelitis treatment due to its biocompatibility, water-containing porous structure, and adaptable physicochemical properties. In this review, we discuss several hydrogel-based strategies for osteomyelitis treatment and categorized them based on the encapsulated cargos (i.e., antibiotics, silver nanoparticles, protein and bacteriophage, and reactive oxygen species (ROS) generator). Several representative examples of osteomyelitis treatment using hydrogels are described here, focusing on their design, preparation, properties, and outcomes. We also provide our perspectives on the remaining concerns regarding fabricating advanced hydrogels for osteomyelitis treatment. This review will be valuable to the hydrogel community and inspire researchers to develop next-generation hydrogels for specific and practical clinical applications in osteomyelitis.

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Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model

Cited by 24 publications

References 72 publications

Alkaline “Nanoswords” Coordinate Ferroptosis-like Bacterial Death for Antibiosis and Osseointegration

Alkaline “Nanoswords” Coordinate Ferroptosis-like Bacterial Death for Antibiosis and Osseointegration

Single atom nanozymes for bacterial infection therapy

Hydrogel-Based Strategies for the Management of Osteomyelitis

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