Fermented Rice Bran Downregulates MITF Expression and Leads to Inhibition of α-MSH-Induced Melanogenesis in B16F1 Melanoma

Chung, So-Young; Seo, Young-Kwon; Park, Jung-Min; Seo, Minjae; Park, Jung-Keug; Kim, Jin-Wook; Park, Chang-Seo

doi:10.1271/bbb.80766

Cited by 65 publications

(48 citation statements)

References 31 publications

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“…It can also increase mitochondrial function in neural cells [9] and displays an inhibitory effect on melanogenesis by downregulation of MITF [10]. Additionally, rice bran possesses many phytochemicals and nutrients, with known chemopreventive and immune-enhancing properties, such as vitamins, γ-oryzanol, tocopherols, tocotrienols, phenolic acids, carotenoids, polysaccharides, phytosterols, essential amino acids, and micronutrients.…”

Section: Introductionmentioning

confidence: 99%

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Kim

Kwon

Jang

et al. 2017

Food & Nutrition Research

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The objective of this study is to evaluate of rice bran mineral extract (RBM) increases the expression of anagen-related molecules in human dermal papilla (DOCs). Four treatment groups were established to evaluate the efficacy of RBM, including a negative control, positive control (ascorbic acid), RBM and ortho-silicic acid (Si(OH)4) (OSA) group. Three days after the DPCs were administered the various treatments, western blot analysis showed that type I collagen expression was increased 2.5-fold in the OSA group and 4-fold in the RBM group, and ALP expression was increased 1.5-fold in the OSA and RBM group while the expression of fibronectin was increased ~3-fold in the OSA group and 2.5-fold in the RBM group. Also, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group. Furthermore, the expression of IL-1a was decreased by more than 50% in the OSA and RBM groups compared to the negative control. Analysis of mRNA expression by RT-qPCR showed that type I collagen increased 1.2-fold in the OSA- and RBM-treated DPCs, whereas type IV collagen increased 2.7-fold in the OSA group and 3.5-fold in the RBM group. However, TGF-β2 mRNA decreased about 80% in the OSA and RBM groups, respectively. Immunohistochemical staining of the DPCs for versican protein showed a significant increase in the OSA- and RBM-treated groups compared to the negative control. Thus, RBM have a potential to recover of DPCs activity and decreased inflammatory-related markers. It can be expected that hair loss prevention and hair growth enhancement can be expected when RBM is applied as a cosmetic product.

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Section: Introductionmentioning

confidence: 99%

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Kim

Kwon

Jang

et al. 2017

Food & Nutrition Research

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“…Melanin content was measured as described previously with minor modification (Chung et al, 2009). B16F10 cells (1  10 5 ) were seeded on 6-well tissue culture plates with various concentrations of pLTA for 24 h. The medium was then replaced with serum-free DMEM containing pLTA and -MSH.…”

Section: Measurement Of Melanin Contentmentioning

confidence: 99%

Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells

Kim¹,

Kim²,

Jung³

et al. 2015

Molecules and Cells

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Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria. Its effects on living organisms are different from those of lipopolysaccharide (LPS) found in Gram-negative bacteria. LTA contributes to immune regulatory effects including anti-aging. In this study, we showed that LTA isolated from Lactobacillus plantarum (pLTA) inhibited melanogenesis in B16F10 mouse melanoma cells. pLTA reduced the cellular activity of tyrosinase and the expression of tyrosinase family members in a dose-dependent manner. The expression of microphthalmia-associated transcription factor (MITF), a key factor in the synthesis of melanin, was also decreased by pLTA. Further, we showed that pLTA activated melanogenesis signaling, such as extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinse (PI3K)/AKT. In addition, the expression of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and HuR, which are important RNA-binding proteins (RBPs), was reduced. pLTA likely degrades MITF via regulation of melanogenic signaling and RNA stability of melanogenic proteins, resulting in the reduction of melanin. Thus, our data suggest that pLTA has therapeutic potential for treating hyperpigmentation disorders and can also be used as a cosmetic whitening agent.

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“…Briefly, the cells were treated with the samples in DMEM containing 10% FBS for 3 d. After concentration by centrifugation, the cell pellets were dissolved in 1 ml of 1 N NaOH at 100℃ for 30 min and centrifuged again for 20 min at 16,000× g. The optical densities of the supernatants were measured at 400 nm using an UV-VIS spectrophotometer [6]. The level of DNA protection was calculated with absorbance and expressed as a percentage, compared to blank group.…”

Section: Genomic Dna Isolationmentioning

confidence: 99%

Effect of Oenanthe javanica Ethanolic Extracts on Antioxidant Activity and Melanogenesis in Melanoma Cells

Kwon

Kim²

2013

Journal of Life Science

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The aim of this study is to investigate the melanogenic effect of Oenanthe javanica ethanolic extracts (OJE) containing quercetin and kaempferol in melanoma cells (B16F1). In order to determine whether OJE inhibits melanin synthesis at the cellular level, the melanoma cells were cultured in the presence of different concentrations of OJE. In the present study, the antioxidant effects of OJE on DPPH radical scavenging, power reduction, lipid peroxidation, and DNA oxidation were evaluated in a cell free system. Furthermore, the effect of OJE on the production of melanin was determined by dopaquinone (DOPA) assay and tyrosinase activity. In addition, the protein expression of tyrosinase, as well as antioxidant enzymes such as superoxide dismutase (SOD)-1, SOD-2 and glutathione reductase (GSH), were examined using Western blot analysis. In this study, it was observed that OJE exhibited an inhibitory effect on lipid peroxidation and blocked the DNA oxidation induced by the hydroxyl radical produced by Fenton's reagent. OJE increased melanin synthesis above 50 μg/ml and tyrosinase activity was detected above 50 μg/ml. In Western blot analysis, OJE increased the expression levels of tyrosinase, SOD-1, SOD-2, and GSH in a dose-dependent manner. These findings indicate that OJE with antioxidant activity can regulate the tyrosinase activity and melanin production in melanocyte, suggesting that it could promote the development of black hair as well as protect skin from oxidative stress.Key words : Melanocyte, Oenanthe javanica, superoxide dismutase, tyrosinase *Corresponding author *Tel : +82-51-890-1511, Fax : +82-51-890-2620 *E-mail : mmkim@deu.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionReactive oxygen species (ROS) are generated as by-products of cellular metabolism, primarily in the mitochondria [1]. Biomolecules such as lipids, proteins, and DNA are damaged by the ROS [11]. In particular, the unpaired electrons of oxygen including superoxide, hydrogen peroxide, hydroxyl radical, and peroxynitrite react to form partially reduced ROS [28]. In recent years, some ROS has been known to modulate enzyme activity as well as transcription factors [2]. ROS generated during melanogenesis have been implicated as cytotoxic molecules in the immune responses of insects against their internal metazoan parasites [15,21]. Melanin produced by melanocyte exists in hair, eyes and skin [26]. Melanoma cell stretch swelling and transfer melanin to neighboring cells such as keratinocyte [27]. It function as to protect human body that melanin absorb UV [3]. Skin gets darken than normal one because melanin formation increases in melanocyte [18]. Melanocyte synthesizes melanin from L-tyrosine through stimulation of such as exposes to UV [17]. Tyrosinase which is rate-li...

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Fermented Rice Bran Downregulates MITF Expression and Leads to Inhibition of α-MSH-Induced Melanogenesis in B16F1 Melanoma

Cited by 65 publications

References 31 publications

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells

Effect of Oenanthe javanica Ethanolic Extracts on Antioxidant Activity and Melanogenesis in Melanoma Cells

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