Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Abstract

The objective of this study is to evaluate of rice bran mineral extract (RBM) increases the expression of anagen-related molecules in human dermal papilla (DOCs). Four treatment groups were established to evaluate the efficacy of RBM, including a negative control, positive control (ascorbic acid), RBM and ortho-silicic acid (Si(OH)4) (OSA) group. Three days after the DPCs were administered the various treatments, western blot analysis showed that type I collagen expression was increased 2.5-fold in the OSA group and 4-fold in the RBM group, and ALP expression was increased 1.5-fold in the OSA and RBM group while the expression of fibronectin was increased ~3-fold in the OSA group and 2.5-fold in the RBM group. Also, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group. Furthermore, the expression of IL-1a was decreased by more than 50% in the OSA and RBM groups compared to the negative control. Analysis of mRNA expression by RT-qPCR showed that type I collagen increased 1.2-fold in the OSA- and RBM-treated DPCs, whereas type IV collagen increased 2.7-fold in the OSA group and 3.5-fold in the RBM group. However, TGF-β2 mRNA decreased about 80% in the OSA and RBM groups, respectively. Immunohistochemical staining of the DPCs for versican protein showed a significant increase in the OSA- and RBM-treated groups compared to the negative control. Thus, RBM have a potential to recover of DPCs activity and decreased inflammatory-related markers. It can be expected that hair loss prevention and hair growth enhancement can be expected when RBM is applied as a cosmetic product.

Assessment of anti-ageing effects of oral choline-stabilized orthosilicic acid on hair, skin and nails: an open label, non-randomized interventional study

“…Thus, it adds to the strength of the hair and improves hair quality in turn. 19 In the present study, hair thickness (in terms of hair count, unit of hair and average hair) increased after 2 months of administration of ch-OSA ® . A 2007 study reported that oral ch-OSA ® improved tensile strength and produced thicker hair.…”

Targeting Wnt/β-Catenin Pathway for Developing Therapies for Hair Loss

“…The compound markedly reduced catagen entry as compared to control [ 72 ]. Several other plant extracts, such as extracts of polygonum multiflorum [ 73 ], Aconitie ciliare tuber [ 75 ], Thuja orientalis [ 76 ], Malva verticilata [ 77 ], and rice bran extract [ 79 ], have been reported to promote mouse skin hair growth or the proliferation of human DPCs via the activation of β-catenin signaling. Our laboratory has reported that topical application of 3-Deoxysappanchalcone (3-DSC), a bioactive constituent of Caesalpinia sappan L. (Leguminosae), increased the expression of cyclin-dependent kinase-4 (Cdk4), FGF, and vascular VEGF, and promoted anagen phase hair growth in C57BL/6 mice via STST3 activation.…”

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

“…The research on herbs and active constituents as hair growth promoters has been driven by their less toxicity, easy availability, low‐cost, patient compliance and more than one mode of biochemical action . Specific effects of herbal extracts, polyherbal formulations, flavonoids and natural compounds on DPCs have been examined . In vivo hair growth promoting effects as proof of concept are also mentioned.…”